Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Controllable escherichia coli acid-resistant system based on ion channel and application thereof

A technology of Escherichia coli and acid resistance, applied in the field of microbial genetic engineering, can solve problems such as ion channel proteins that are not suitable for acid resistance, and achieve the effect of ensuring cell survival

Inactive Publication Date: 2015-03-25
PEKING UNIV
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the system can expel intracellular H at a sufficiently fast rate + , to maintain the intracellular pH value in which E. coli cells can survive, so that the functions of other ion channels cannot show an impact on the acid-resistant survival rate, so it is not suitable for artificial regulation and control of the acid-resistant ability of E. coli and the determination of substrates for ion channel proteins and function

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Controllable escherichia coli acid-resistant system based on ion channel and application thereof
  • Controllable escherichia coli acid-resistant system based on ion channel and application thereof
  • Controllable escherichia coli acid-resistant system based on ion channel and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023]Below in conjunction with the accompanying drawings, the present invention will be further described through the embodiments, but the protection scope of the present invention will not be limited thereby.

[0024] 1. Strain construction with controllable ion channel acid resistance system

[0025] 1) Obtaining ΔgadA / B double mutant strain BD9025

[0026] The genes gadA and gadB encoding glutamate decarboxylases GadA and GadB were deleted from the genome of wild-type E. coli MG1655 (from ATCC, USA). The specific method is as follows:

[0027] ΔgadA::kan was introduced into MG1655 from the existing donor strain JW3485-1 (provided by the Escherichia coli Genetic Stock Center (CGSC) at Yale University, USA) by P1 phage transduction, and then passed site-specific Then, ΔgadB::kan was transduced from the donor strain JW1488-7 (from CGSC) into this strain through P1 phage, and the kan gene was removed again by site-specific recombination to obtain ΔgadA / B double mutant stra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a controllable escherichia coli acid-resistant system based on an ion channel and application thereof. The original glutamine-mediated acid-resistant system of escherichia coli is based, and the survival rate of escherichia coli is enabled to be related to the expression quantity of ammonium ion channel proteins and the concentration of ammonium ions in a culture medium under an extreme acidic condition with glutamine by knocking out glutamate decarboxylase GadA and GadB in the original system, so that the acid resistance of escherichia coli can be controlled by regulating the concentration of extracellular ammonium ions and / or the expression quantity of intracellular ammonium ion channel proteins, thus controlling the survival of escherichia coli under the acidic condition. The acid-resistant system can also be used as a mode system for measuring the substrate and functions of the ion channel proteins.

Description

technical field [0001] The invention relates to an acid resistance system of Escherichia coli, in particular to a method for controlling the acid resistance of Escherichia coli through a controllable ion channel-based acid resistance system, and belongs to the technical field of microbial genetic engineering. Background technique [0002] Acid resistance is a very important property of Escherichia coli (E. coli), because intestinal bacteria must survive the extremely acidic environment in the stomach before they can enter the small intestine. [0003] It is known that Escherichia coli has four inducible acid resistance systems to resist strong acidic environment. Among them, the second acid resistance system (acid resistance system2, AR2) needs to use glutamic acid (Glu) or glutamine (Gln) in the medium, corresponding to the acid resistance system (Glu-dAR) dependent on glutamic acid, respectively. ) and glutamine-dependent acid resistance system (Gln-dAR). The known main ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21
CPCC12N9/88C12Y401/01015
Inventor 刘歆王吉龙杨建国王忆平
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com