Method for measuring virulence by feeding Bemisia tabaci Gennadius imago
A technology for bemisia tabaci and adults, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of mesophyll damage and withering, easy mold of agar, high probability of bemisia tabaci sticking to agar, etc., to achieve The effect of avoiding death, prolonging the fresh-keeping period, and avoiding non-toxic death
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[0019] Example 1: (Method for virulence determination of adult Bemisia tabaci from Euphorbia euphorbiae)
[0020] (1) Preparation and sterilization of synthetic medium: weigh 7g of agar, heat, stir and dissolve in 500mL of distilled water to prepare agar solution; then add 25mL of NH 4 NO 3 20mg / L, KNO 3 3mg / L, MgSO 4 ·7H 2 O2mg / L, KH 2 PO 4 2mg / L, anhydrous CaCl 2 1mg / L and make up to 1 liter of nutrient solution with water, stir evenly to form a synthetic medium; after autoclaving the synthetic medium at 121°C for 15-20 minutes, pour it separately on a clean bench Put into a sterilized flat-bottomed glass test tube of length×diameter×thickness of 30mm×25mm×1.5mm. After cooling, put the glass test tube into a sterilized and dried wide-mouth glass bottle and seal it with sterile plastic paper for use. ;
[0021] (2) Medicinal treatment, petiole disinfection and insertion of leaves: Cut the fresh leaves of Euphorbia pulcherrima, and keep the petiole length 30-40mm; the leaves are ma...
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[0024] Example 2: (Method for virulence determination of adult Bemisia tabaci on eggplant)
[0025] In this example, the preparation of step (1) synthetic medium is: weigh 7.5g of agar and dissolve it in 500mL of distilled water to prepare an agar solution; then add 27.5mL of NH 4 NO 3 22.5mg / L, KNO 3 2mg / L, MgSO 4 ·7H 2 O 1mg / L, KH 2 PO 4 3mg / L, anhydrous CaCl 2 2mg / L and make up to 1 liter of nutrient solution with water, stir evenly to prepare a synthetic medium; Step (2) Leaf medical treatment, petiole disinfection and insertion: Cut the test material eggplant leaves with 10% imidacloprid WP 2000 After the multiple dilution solution is processed and dried in the shade, the petiole is immersed in a 50% carbendazim wettable powder 800-fold dilution for 10-20s for disinfection; the remaining steps and processes are the same as in Example 1.
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[0026] Example 3: (Method for virulence determination of adult Bemisia tabaci on cotton)
[0027] In this example, the preparation of step (1) synthetic medium is: weigh 8g of agar and dissolve it in 500mL of distilled water to prepare an agar solution; then add 30mL of NH 4 NO 3 25mg / L, KNO 3 1mg / L, MgSO 4 ·7H 2 O3mg / L, KH 2 PO 4 1mg / L, anhydrous CaCl 2 3mg / L and make up to 1 liter of nutrient solution with water, stir evenly to prepare a synthetic medium; step (2) leaf medicament treatment, petiole disinfection and insertion: cut specimen cotton leaves with 10% imidacloprid WP 3000 After the multiple dilution solution is processed and dried in the shade, the petiole is immersed in a 1000-fold dilution solution of 50% carbendazim wettable powder for 10-20 seconds for disinfection; the remaining steps and processes are the same as in Example 1.
[0028] Table 1 Mold on synthetic medium and preservation of leaves
[0029]
[0030] Note: The curl on the edge of the leaf is also recorde...
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