Ion channel medicament screening apparatus and method

An ion channel and screening method technology, applied in the field of ion channel drug screening devices, can solve problems such as low success rate, and achieve the effect of improving the success rate

Inactive Publication Date: 2009-08-05
SHENZHEN INST OF ADVANCED TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0008] In view of this, it is necessary to provide an ion channel drug screening m

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  • Ion channel medicament screening apparatus and method
  • Ion channel medicament screening apparatus and method
  • Ion channel medicament screening apparatus and method

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Embodiment Construction

[0033] In the following ion channel drug screening device and method, the light-sensitive ion channel protein is introduced into cells, so that the switch of the ion channel can be controlled by light. Firstly, a certain wavelength of light is applied to open or close the ion channel, and the transmembrane electrical signal of the cell is recorded through the patch clamp; then the cells in different holes of the chip are given different drugs to be screened through the drug delivery system, and the corresponding transmembrane electrical signal is recorded. Changes in membrane electrical signals; analysis based on changes in electrical signals to screen out effective drugs.

[0034] Different light-sensitive ion channel proteins are sensitive to light of different wavelengths, which can control the entry of different ions into cells accordingly. like figure 1 As shown, ChR2 (Channelrhodopsin-2) protein is sensitive to blue light (472nm wavelength), and blue light can make Na ...

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Abstract

An ion channel drug screening method includes the following steps of affusing cells with photosensitive ion channel protein into a chip; performing light treatment to the cells with photosensitive ion channel protein, which is affused into the chip; recording a reference transmembrane electric signal; administration; recording the transmembrane electric signal after administration; analyzing the reference transmembrane electric signal before administration and an administration transmembrane electric signal after administration and judging the affect of a drug to ion channels. Controlling the ion channels by light, the ion channel drug screening method can uniformly control the switches of the ion channels of the cell and facilitate accurately knowing the affect of the drug to the ion channels, thereby improving the success ratio of ion channel drug screening. In addition, the invention also provides an ion channel drug screening device.

Description

【Technical field】 [0001] The invention relates to drug screening technology, in particular to an ion channel drug screening device and method. 【Background technique】 [0002] Ion channel proteins are macromolecular membrane proteins that regulate the level of intracellular and extracellular ions. The abnormality of their structure and function may lead to the occurrence of various diseases. Using ion channels as drug targets can treat diseases such as hypertension, arrhythmia, epilepsy, senile dementia, neuralgia, diabetes, and hyperkalemic periodic paralysis. However, ion channel drugs only account for 7% of all drugs on the market at present, and the development of such drugs awaits the advent of new high-throughput drug screening technologies. [0003] Traditional ion channel drug screening uses the patch clamp technique. Patch clamp is the best method for ion channel drug screening and ion channel research, but because it is only applicable to a single cell, the throug...

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Application Information

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IPC IPC(8): G01N33/566
Inventor 祁晖屠洁刘楠王立平
Owner SHENZHEN INST OF ADVANCED TECH
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