A method suitable for rapid classification and counting of fish blood cells and its application

A rapid classification and blood cell technology, applied in the field of detection, to achieve the effect of good batch repeatability, easy access, and ensure accuracy and reliability
CN107686859BActive Publication Date: 2021-07-30TIANJIN NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN NORMAL UNIVERSITY
Publication Date
2021-07-30

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Abstract

The invention discloses a high-throughput rapid classification and analysis method for blood cells suitable for common fish such as crucian carp and carp. Aiming at the problem that nucleated red blood cells that cannot be removed interfere with white blood cell classification and counting in terms of quantity and shape, an automated measurement and analysis strategy combining fluorescent dyes and multi-channel images is used to separate the interference The red blood cell samples are separated from the white blood cells, and the range of different white blood cell groups that undertake important immune functions such as lymphocytes, monocytes, and granulocytes is divided, and automatic analysis is completed. The difficulty of effectively removing nucleated red blood cells has long plagued the automatic analysis of blood images of many types of animals. This invention combines fluorescent dyes with image analysis, and uses the high-throughput and high-sensitivity measurement modules of the multi-dimensional panoramic analyzer to accurately identify red blood cell groups. Eliminating its interference provides important technical support for focused analysis of leukocyte groups and has broad application prospects.
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Description

technical field

[0001] The invention belongs to the technical field of detection, and relates to a method for analyzing the types, quantities and proportions of various blood cells in crucian carp whole blood by using a novel automatic cell detection technology combining fluorescent dyes and multi-channel images. The method is simple, fast, accurate, and high-throughput detection of crucian carp blood cell types and quantitative ratios, and can perform more accurate and efficient automatic analysis of crucian carp blood cells without effective cleavage reagents, avoiding heavy manual classification and counting, and Manual identification differences lead to "different" result deviations, which solves the main problem of interference with white blood cell classification and counting caused by a large number of red blood cells that cannot be removed, and realizes a rapid "blood test" for fish. Background technique

[0002] Hemogram refers to the general term of the general tes...

Claims

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