Detection chip for tumor driving gene and application thereof

A detection chip and driver gene technology, applied in the biological field, can solve the problems of difficult detection and low sensitivity of tumor driver genes

Active Publication Date: 2017-08-08
常州桐树生物科技有限公司
View PDF8 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection products have low sensitivity and are generally only designed for the mutation or amplific...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection chip for tumor driving gene and application thereof
  • Detection chip for tumor driving gene and application thereof
  • Detection chip for tumor driving gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] 236 probes of sequences shown in SEQ ID No.1 to SEQ ID No.236 are arranged on the detection chip, and the venous blood of patients with non-small cell lung cancer is detected as 12 samples (provided by Jinhua Hospital of Traditional Chinese Medicine) with the detection chip. The 12 tumor driver genes are ALK, BRAF, DDR2, EGFR, ERBB2, FGFR1, KRAS, MET, NRAS, PIK3CA, RET and ROS1. The sequencing platform is Illumina, the reference genome is GRCh37 / hg19, and the reference transcriptome is Ensembl75. The reference databases are COSMIC (version v74), dbSNP (version v142), iCMDB (version 4.0.0). The filter conditions are as follows:

[0151] 1. Keep the variants that meet the following filter conditions: a. Base quality > 20, b. Sequence alignment quality > 50, c. Strand preference = 30, f. The number of sequencing short fragments supporting SNP variation in the sample >= 3, g. The number of sequencing short fragments supporting Indel variation in the sample >= 5.

[0152] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detection chip for a tumor driving gene and application thereof. The detection chip for the tumor driving gene comprises an ALK (anaplastic lymphoma kinase) fusion detection agent, an EGFR (epidermal growth factor receptor) fusion detection agent, an RET (reticulocyte) fusion detection agent and an ROS1 (reactive oxygen species 1) fusion detection agent. Proofed by the results of clinical detect, after fusion probes corresponding to the ALK, the EGFR, the RET and the ROS1 are specifically designed, the detection chip has the advantages that the sensitivity is high, and the gene fusion between a particular site area of the ALK, the EGFR, the RET and the ROS1 and fusion segments is specifically detected. The invention further discloses a detection chip for detecting a second sequence group of the gene mutation and a third sequence group of the gene amplification; after detecting once, the gene fusion, gene mutation and gene amplification of the multiple tumor driving genes, such as the ALK, BRAF (aserine/theroninespecific kinases), DDR2 (discordin domain receptor 2), the EGFR, ERBB2 (receptor tyrosine kinase 2), FGFR1 (fibroblast growth factor receptor 1), KRAS (kirstenrat sarcoma viral oncogene), MET (methionine), NRAS, PIK3CA (phosphatidylino-sitol 3-kinases), the RET and the ROS1.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tumor driver gene detection chip and its application. Background technique [0002] Tumors are a group of malignant diseases caused by driver genes, which are characterized by high morbidity and high mortality. Common tumor driver genes include ALK gene, BRAF gene, DDR2 gene, EGFR gene, ERBB2 gene, FGFR1 gene, KRAS gene, MET gene, NRAS gene, PIK3CA gene, RET gene and ROS1 gene, etc. In the past 10 years, the incidence of tumors in my country has increased rapidly, seriously endangering public health. Clinical studies have found that about 87% of patients have known driver genes, of which about 81% of driver genes have clear targeted inhibitors, and 66% of patients can receive individualized targeted therapy with marketed drugs. The results of the study showed that by detecting the driver gene and receiving targeted therapy, the patient's survival time can be extended by at least ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C40B40/06
CPCC12Q1/6886C12Q2600/106C12Q2600/156C40B40/06
Inventor 严令华韩宁宁
Owner 常州桐树生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap