A nucleic acid fluorescent probe for nuclear staining and its preparation method

Abstract

The invention discloses a preparation method of a nucleic acid fluorescence probe compound AzosD for nuclear staining. The preparation method is characterized by comprising steps as follows: 6-amino-2-methylquinoline is dissolved in acetonitrile, N-bromosuccinimide is added, a reaction is performed, and an intermediate 1 is obtained; the intermediate 1 is dissolved in a water, ethanol and toluenemixed solution, 3-(N,N-dimethylamino)phenylboronic acid, tetrakis(triphenylphosphine)palladium and sodium carbonate are added, refluxing reaction is performed under nitrogen protection, and an intermediate 2 is obtained; the intermediate 2 is dissolved by hydrochloric acid, sodium nitrite is added to the solution under the condition of ice-water bath, pH of the solution is regulated to be neutralby a sodium hydroxide aqueous solution, and 3-methyl-11-(N,N-dimethylamino) cinnoline[3,4-e]quinoline, namely AzosD, is obtained by separation and purification. The compound is good in photobleachingresistant effect, high in biocompatibility, high in enzyme-resistant capacity and high in nuclear targeting, can be applied to specific staining of nucleuses of living cells, and has broad applicationprospect in the fields of long-term monitoring of nuclear morphometry and observation for nuclear morphology change in the cellular physiological process.

Description

technical field

[0001] The invention belongs to the technical field of cell nucleus fluorescent probes, in particular to a nucleic acid fluorescent probe 3-methyl-11-(N,N-dimethylamino)cinnolino[3,4-e]quinone used for cell nucleus staining Phenyl (AzosD) and its preparation method. Background technique

[0002] With the development of science and technology, human beings have a deeper understanding of the nucleus. The nucleus is the largest and most important organelle in eukaryotic cells, and it is the regulatory center of cell inheritance and metabolism. The nucleus is the control center of the cell, plays an important role in the metabolism, growth and differentiation of the cell, and is the main location of genetic material. Nucleic acid is a biological macromolecule closely related to life activities and diseases. Therefore, the staining, tracking and morphological observation of the nucleus are of great significance for understanding the life process of the whole ce...

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