Closed nucleic acid chromatographic test paper detection kit preserved at normal temperature and detection method

A technology for preservation at room temperature and chromatography test paper, which is applied in the field of harmless treatment of nucleic acid amplification products and preservation of nucleic acid amplification reagents at room temperature, can solve the problems of limited application, increased difficulty and cost of injection molding production, and many steps, etc. Simplified extraction steps and time, shortened extraction steps and time, chemically stable effect

Inactive Publication Date: 2013-08-14
QUICKING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods and measures require strict experimental conditions and expensive experimental facilities and equipment, and are not suitable for popularization to grassroots users.
The second is to use dUTP instead of dTTP in the nucleic acid amplification reaction, and add UNG enzyme to degrade the uracil base in the contaminated product U-DNA, and completely hydrolyze and break the U-DNA chain under high temperature denaturing conditions to eliminate Due to the amplification caused by the contaminated product, the specificity of the amplification result is guaranteed, but this method requires variable temperature operation, many steps, high cost, and there is a certain probability of contamination, which limits its application
Gu Jiayong et al. (CN1888902) invented a closed nucleic acid lateral flow chromatography detection device, which can improve detection sensitivity and initially solve the problem of amplification product contamination
However, the disadvantages of this device are: 1. The device is large in size and is not suitable for large-scale production and transportation of finished products; 2. The test strips in the device are complicated to install and are not suitable for large-scale test paper assembly and production; 3. After the device is restarted and used , can be reopened easily and without traces, so the nucleic acid amplification product is only relatively closed. If the device that has been reacted is opened unintentionally, the contamination of the amplification product is inevitable; 4. The device In this method, only a single reaction solution can be detected, but multiple reaction solutions cannot be detected, that is, the detection of multiple samples cannot be completed on one test strip in the form of multiple reaction solutions
This is not conducive to environmental protection, but also increases the difficulty and cost of injection molding production

Method used

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  • Closed nucleic acid chromatographic test paper detection kit preserved at normal temperature and detection method
  • Closed nucleic acid chromatographic test paper detection kit preserved at normal temperature and detection method
  • Closed nucleic acid chromatographic test paper detection kit preserved at normal temperature and detection method

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Embodiment 1

[0109] like figure 1 As shown, the present invention includes a device assembled from a plurality of components to guide liquid to flow to a lateral flow chromatography test paper in a closed space for detection reaction. The three components are stackable sealable reaction solution tube (hereinafter referred to as solution tube 1), sealable connecting tube (hereinafter referred to as connecting tube 2), and sealable test paper tube (hereinafter referred to as test paper tube 3). Solution tube 1 can be one or more, in figure 1 In the shown embodiment, two solution tubes 1 are used.

[0110] like figure 2 As shown, the solution tube 1 includes a solution tube body 11 and a solution tube cover 12, and the solution tube body 11 and the solution tube cover 12 are connected by a solution tube connecting bar 13; the solution tube body 11 includes the bottom of the solution tube from bottom to top Protrusion 111, solution pipe external groove 112, solution pipe internal groove 11...

Embodiment 2

[0128] When two kinds of test solutions are required to be tested after mixing and reacting, two solution pipes need to be stacked, and the device of the present invention can adopt another structure, such as Figure 4 As shown, a counterbore-shaped cavity 125 can be established on the top of the solution tube cover 12, and another solution tube can be inserted in this counterbore-shaped cavity 125 (see Figure 9 ); the bottom of the counterbore-shaped cavity 125 of the solution tube cover 12 is provided with a cut sheet 123 for the solution tube cover and a pit 124 for inserting and fitting the protrusion 111 at the bottom of the solution tube. The protrusion 111 at the bottom of the solution tube and the pit 124 are in-line or cross-shaped or other shapes of mutual fitting structure; the outer circumference of the solution tube cover 12 is provided with a raised sealing ring 121 on the outer circumference of the solution tube cover near the bottom of the cover, and the soluti...

Embodiment 3

[0140] Such as Figure 11-13As shown, the difference between this embodiment 3 and embodiment 1 is: the bottom structure of the solution tube is provided with a bottom cut 114, but there is no bottom protrusion; The solution channel hole 25B is composed of the raised part 25A against the bottom structure of the solution tube. When the bottom structure of the solution tube is subjected to the force of the raised part 25A against the top, it breaks away from the bottom cut 114. At this time, it is pre-packed in the solution tube. The sample solution in the tube 1 can flow out from the fracture gap, and flow to the sample solution receiving area of ​​the chromatography test paper in the test paper tube through the solution channel hole 25B at the bottom of the connecting tube; the cut mark 114 at the bottom of the solution tube is a line or point shape, the material thickness of the cut part is 0.05-0.5mm less than that of other parts; when the bottom structure of the solution tu...

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Abstract

The invention discloses a method for preserving nucleic acid amplification reagents at normal temperature. Partial nucleic acid amplification reagents or all reagents are converted to solid state through a solidifying process and preserved stably at normal temperature. The invention further discloses a closed nucleic acid chromatographic test paper detection kit preserved at normal temperature. The kit comprises a nucleic acid extraction reagent of a sampler, the nucleic acid amplification reagent preserved at normal temperature, a closed chromatographic test paper detection device and an innocent treatment method of nucleic acid amplification products. The closed chromatographic test paper detection device comprises immunochromatographic test paper and a closed detection device, wherein the immunochromatographic test paper is fixedly arranged in the closed detection device, and sample liquor detected cannot be contacted with external environment. In addition, the invention further discloses a method for nucleic acid detection by using the kit. According to the invention, the nucleic acid reagents can be conveyed and preserved at normal temperature and extracted simply and quickly, and the nucleic acid amplification products are hermetically, quickly and sensitively detected, so that cross infection is avoided.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a method for storing nucleic acid amplification reagents at normal temperature; in addition, the invention also relates to a closed nucleic acid chromatography test paper detection kit and a detection method thereof for storage at normal temperature, and also discloses nucleic acid amplification The harmless disposal method of the product. Background technique [0002] Since 1985, the American Cetus company used Polymerase Chain Reaction (Polymerase Chain Reaction, PCR) to realize the specific amplification of nucleic acid, this technology has been applied to medical in vitro diagnosis and medical treatment because of its high sensitivity, high specificity and high efficiency. Food safety testing and other fields. In recent years, with the rapid development of molecular biology and the extensive application of biophysical techniques, new nucleic acid amplification modes are also emerging. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/10
Inventor 周中人林忠旺熊丁杰周正峰蒋庭吴海洋
Owner QUICKING BIOTECH
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