Spinal cord needle biopsy tissue fixation decalcification solution, preparation method and decalcification method

Abstract

The invention discloses a spinal cord needle biopsy tissue fixation decalcification solution, a preparation method and a decalcification method. The spinal cord needle biopsy tissue fixation decalcification solution is prepared from the following components in parts by weight: 30-35 parts of formaldehyde, 300-400 parts of methanol, 400-500 parts of an ethylenediamine tetraacetic acid saturated solution, 7-9 parts of a PBS buffer solution, 25-35 parts of an accelerant, 1-3 parts of sodium chloride and 100-150 parts of water. The decalcification solution is small in tissue damage and better in tissue integrity, meanwhile, the decalcification time is greatly shortened, the decalcification time can be shortened from traditional 72 h to 24 h, the requirement for rapidly obtaining a pathological diagnosis report is greatly met, meanwhile, excimer detection can be conducted, accurate medication can be conducted on later-stage targeted therapy, the diagnosis efficiency is improved, and after decalcification treatment, the staining effect of a tissue specimen is better, the structure is clearer, the structures of soft tissues and bone groups in the section are completely displayed, the cell nucleus staining is clear, the nucleoplasm contrast is distinct, and the method has higher application value in the spinal cord needle biopsy tissue sheet preparation.

Description

technical field [0001] The invention belongs to the technical field of pathological detection, and in particular relates to a spinal cord biopsy tissue fixation decalcification solution, a preparation method and a decalcification method. Background technique [0002] Bone marrow biopsy is one of the important components of pathological examination, and its diagnostic report is an important basis for the diagnosis and treatment of diseases in clinical departments such as hematology. Bone tissue is very common in bone marrow biopsy. Due to the hard texture of bone tissue, if the calcium in it cannot be effectively removed, it is difficult for the pathology laboratory to make high-quality slices or slices directly. Therefore, decalcification of tissue samples is also That is, the softening treatment of tissue specimens becomes the key to making such slices. How to fix and decalcify the specimen in a short period of time, and at the same time make the slices the same as the rou...

AI Technical Summary

Problems solved by technology

Nitric acid causes excessive damage to proteins and nucleic acids in tissues, seriously affecting subsequent tissue staining results, and causing false negative results in subsequent immunohistochemical and molecular pathological tests

Nitric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety hazards to the safety of the environment and operators.

2. Hydrochloric acid decalcification solution: faster decalcification, no tissue fixation

Hydrochloric acid causes too much damage to proteins and nucleic acids in tissues, which affects the subsequent tissue staining effect and affects the reliability of subsequent immunohistochemical and molecular pathological detection results

Hydrochloric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety hazards to the safety of the environment and operators.

3. Sulfuric acid decalcification solution: fast decalcification, no tissue fixation

However, because EDTA is a chelating agent for decalcification through chelation reaction, the decalcification time takes 3-4 weeks, or even longer, and it is difficult to be widely used

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