Spinal cord needle biopsy tissue fixation decalcification solution, preparation method and decalcification method

A technique of puncture biopsy tissue and preparation method, which is applied in the field of pathological detection, can solve the problems affecting the reliability of immunohistochemical detection and molecular pathological detection results, hidden dangers of environment and operator safety and security, and has no tissue fixation effect, and achieves staining. The effect is better, the decalcification time is shortened, and the effect of meeting the requirements of the pathological diagnosis report

Inactive Publication Date: 2021-07-09
宁波同盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitric acid causes excessive damage to proteins and nucleic acids in tissues, seriously affecting subsequent tissue staining results, and causing false negative results in subsequent immunohistochemical and molecular pathological tests
Nitric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety hazards to the safety of the environment and operators.
2. Hydrochloric acid decalcification solution: faster decalcification, no tissue fixation
Hydrochloric acid causes too much damage to proteins and nucleic acids in tissues, which affects

Method used

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  • Spinal cord needle biopsy tissue fixation decalcification solution, preparation method and decalcification method
  • Spinal cord needle biopsy tissue fixation decalcification solution, preparation method and decalcification method

Examples

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Embodiment 1

[0031] A solution for fixing and decalcifying spinal cord biopsy tissue, which is made of the following ingredients in parts by weight: 32 parts of formaldehyde, 350 parts of methanol, 450 parts of saturated ethylenediaminetetraacetic acid solution, 8 parts of PBS buffer solution, 30 parts of accelerator, chlorinated 2 parts of sodium, 128 parts of water.

[0032] The preparation method of described EDTA saturated liquid is as follows:

[0033] (1) Mix ethylenediaminetetraacetic acid and water at a mass ratio of 13:100 and stir for 10 minutes;

[0034] (2) Send the above mixed solution into a constant temperature box and keep it at 60°C for 10 hours;

[0035] (3) Take it out and store it at room temperature.

[0036] The accelerator is prepared by mixing aluminum chloride, chloroform, and polyethylene glycol octylphenyl ether in a mass ratio of 17:11:2.

[0037] The preparation method of described spinal cord biopsy tissue fixation decalcification solution comprises the fol...

Embodiment 2

[0046] A spinal cord biopsy tissue fixation decalcification solution, which is made of the following ingredients in parts by weight: 30 parts of formaldehyde, 300 parts of methanol, 400 parts of ethylenediaminetetraacetic acid saturated solution, 7 parts of PBS buffer solution, 25 parts of accelerator, chlorinated 1 part sodium, 100 parts water.

[0047] The preparation method of described EDTA saturated liquid is as follows:

[0048] (1) Mix ethylenediaminetetraacetic acid and water at a mass ratio of 13:100 and stir for 10 minutes;

[0049] (2) Send the above mixed solution into a constant temperature box and keep it at 60°C for 10 hours;

[0050] (3) Take it out and store it at room temperature.

[0051] The accelerator is prepared by mixing aluminum chloride, chloroform, and polyethylene glycol octylphenyl ether in a mass ratio of 17:11:2.

[0052] The preparation method of described spinal cord biopsy tissue fixation decalcification solution comprises the following steps...

Embodiment 3

[0061] A spinal cord biopsy tissue fixation decalcification solution, which is made of the following ingredients in parts by weight: 35 parts of formaldehyde, 400 parts of methanol, 500 parts of ethylenediaminetetraacetic acid saturated solution, 9 parts of PBS buffer solution, 35 parts of accelerator, chlorinated 3 parts of sodium, 150 parts of water.

[0062] The preparation method of described EDTA saturated liquid is as follows:

[0063] (1) Mix ethylenediaminetetraacetic acid and water at a mass ratio of 13:100 and stir for 10 minutes;

[0064] (2) Send the above mixed solution into a constant temperature box and keep it at 60°C for 10 hours;

[0065] (3) Take it out and store it at room temperature.

[0066] The accelerator is prepared by mixing aluminum chloride, chloroform, and polyethylene glycol octylphenyl ether in a mass ratio of 17:11:2.

[0067] The preparation method of described spinal cord biopsy tissue fixation decalcification solution comprises the follow...

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Abstract

The invention discloses a spinal cord needle biopsy tissue fixation decalcification solution, a preparation method and a decalcification method. The spinal cord needle biopsy tissue fixation decalcification solution is prepared from the following components in parts by weight: 30-35 parts of formaldehyde, 300-400 parts of methanol, 400-500 parts of an ethylenediamine tetraacetic acid saturated solution, 7-9 parts of a PBS buffer solution, 25-35 parts of an accelerant, 1-3 parts of sodium chloride and 100-150 parts of water. The decalcification solution is small in tissue damage and better in tissue integrity, meanwhile, the decalcification time is greatly shortened, the decalcification time can be shortened from traditional 72 h to 24 h, the requirement for rapidly obtaining a pathological diagnosis report is greatly met, meanwhile, excimer detection can be conducted, accurate medication can be conducted on later-stage targeted therapy, the diagnosis efficiency is improved, and after decalcification treatment, the staining effect of a tissue specimen is better, the structure is clearer, the structures of soft tissues and bone groups in the section are completely displayed, the cell nucleus staining is clear, the nucleoplasm contrast is distinct, and the method has higher application value in the spinal cord needle biopsy tissue sheet preparation.

Description

technical field [0001] The invention belongs to the technical field of pathological detection, and in particular relates to a spinal cord biopsy tissue fixation decalcification solution, a preparation method and a decalcification method. Background technique [0002] Bone marrow biopsy is one of the important components of pathological examination, and its diagnostic report is an important basis for the diagnosis and treatment of diseases in clinical departments such as hematology. Bone tissue is very common in bone marrow biopsy. Due to the hard texture of bone tissue, if the calcium in it cannot be effectively removed, it is difficult for the pathology laboratory to make high-quality slices or slices directly. Therefore, decalcification of tissue samples is also That is, the softening treatment of tissue specimens becomes the key to making such slices. How to fix and decalcify the specimen in a short period of time, and at the same time make the slices the same as the rou...

Claims

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Application Information

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IPC IPC(8): G01N1/36G01N1/31
CPCG01N1/31G01N1/36G01N2001/366
Inventor 尤丽丽
Owner 宁波同盛生物科技有限公司
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