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Pap staining solution and application method

A pasteurized dyeing and dyeing liquid technology, applied in the biological field, can solve the problems of complex pasteurized color liquid configuration, affecting pathological diagnosis, complicated dyeing procedures, etc. Post-color stabilization effect

Inactive Publication Date: 2018-09-14
南京福怡科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional Papanicolaou color solution is complicated to configure, the staining procedures are complicated, and the staining time is long. The staining result is affected by various factors such as staining time, rinsing time, and rinsing times. The effect is unstable and affects the judgment of pathological diagnosis.

Method used

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  • Pap staining solution and application method
  • Pap staining solution and application method

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Improved hematoxylin staining solution 1, the components of each liter of the staining solution are: methanol 100ml, glacial acetic acid 20ml, hematoxylin 2g, aluminum potassium sulfate 10g, sodium iodate 0.1g, purified water 870ml.

[0020] OG-EA staining solution 1, the components of each liter of dyeing solution are: bright green 0.5g, eosin yellowish 2.5g, absolute ethanol 500ml, phosphotungstic acid 1g, ethanol 200ml, glacial acetic acid 20ml, phosphate buffer 12ml, Orange 3g, purified water 265ml.

[0021] Dyeing method:

[0022] 1) After the cytological preparation is completed, wait for wet fixation;

[0023] 2) After washing with running water, use the above-mentioned modified hematoxylin staining solution 1 to stain the nucleus for 1.5 minutes;

[0024] 3) After washing with running water, use the above OG-EA staining solution 1 to stain the cytoplasm for 2.5 minutes;

[0025] 4) After washing with gradient alcohol, dehydrating and drying, seal the slides; ...

Embodiment 2

[0029] Improved hematoxylin staining solution 2, the components of each liter of the staining solution are: 120 ml of propanol, 30 ml of glacial acetic acid, 3 g of hematoxylin, 15 g of potassium aluminum sulfate, 0.2 g of sodium iodate, and 835 ml of purified water.

[0030] OG-EA staining solution 2, the components of each liter of dyeing solution are: bright green 0.6g, eosin yellowish 3g, absolute ethanol 600ml, phosphotungstic acid 2g, isopropanol 300ml, glacial acetic acid 25ml, Tirs buffer 20ml , orange 4g, purified water 46ml.

[0031] Dyeing method:

[0032] 1) After the cytological preparation is completed, wait for wet fixation;

[0033] 2) After washing with running water, use the above-mentioned modified hematoxylin staining solution 2 to stain the cell nuclei for 1 minute;

[0034] 3) After washing with running water, use the above OG-EA staining solution 2 to stain the cytoplasm for 2 minutes;

[0035] 4) After washing with gradient alcohol, dehydrating and d...

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Abstract

The invention discloses a Pap staining solution and application thereof, and belongs to the field of biotechnology. Specifically, the Pap staining solution contains a cell nuclear staining solution and a cytosolic staining solution. The Pap staining solution provided by the invention is simple to operate, and ensures uniform staining, clear cell structure after staining and bright cell colors, soas to facilitate diagnostic observation and judgment of clinical pathology.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Papanicolaou staining solution, and simultaneously the invention also relates to an application method of the staining solution. Background technique [0002] In the current disease diagnosis process, pathological diagnosis is an important source of diagnostic information and has important clinical significance. Papanicolaou staining is the best and most commonly used staining method in pathological sections and exfoliated cells. This staining method not only has the characteristics of clear nucleus structure, obvious color separation, good transparency, bright cytoplasm, etc., but also can process After that, the specimen is not easy to decolorize and can be stored for a long time. Papanicolaou staining reagents include hematoxylin staining solution, orange staining solution, EA50 staining solution, and 95% ethanol, 100% ethanol, absolute ethanol, etc.; the Papanicolaou staining ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 姚斌左露露
Owner 南京福怡科技发展股份有限公司
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