Multifunctional plant expression vector as well as construction method and application thereof

Abstract

The invention discloses a multifunctional plant expression vector as well as a construction method and application thereof, and belongs to the technical field of genetic engineering. The multifunctional plant expression vector is Pesnoo-eYGFPur-apply, and the nucleotide sequence is shown in SEQ ID NO.1. Due to the addition of upstream and downstream regulatory elements of a target gene, the expression of the target gene can be significantly enhanced compared to a common overexpression vector driven by a CaMV 35S promoter, and at the same time, due to the fusion expression of the target gene and eYGFPuv, the expression of the eYGFPuv is also significantly enhanced; during subcellular localization observation, compared with an ordinary subcellular localization vector, the target gene and eYGFPuv fusion protein in cells for transforming the vector has higher brightness, so that the localization result is more conveniently observed; and the vector is used for plant genetic transformation,when a positive test is performed, people only needs a UV flashlight to irradiate a tissue sample or a plant sample to judge whether the sample is positive by observing the presence or absence of excited green fluorescence, so that the workload of molecular testing of transgenic samples is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a multifunctional plant expression vector and its construction method and application. Background technique [0002] In plant molecular biology and functional gene research, gene overexpression and subcellular localization techniques are widely used. Usually, gene overexpression research needs to insert the target gene sequence into the plant overexpression empty vector to construct a recombinant overexpression plasmid vector; subcellular localization research needs to insert the target gene sequence into the subcellular localization empty vector to construct a recombinant subcellular localization plasmid Vector; Overexpression and subcellular localization studies need to construct corresponding vectors separately, which is time-consuming, laborious and expensive. [0003] The use of multifunctional vectors will greatly reduce the workload of vector construction, sav...

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned existing studies on plant subcellular localization, the expression level of GFP on the carrier is low, and the detection of transgenic samples in plant transgenics has the defects of long time consumption and a large amount of manpower and material resources. The purpose of the present invention is to provide a The multifunctional plant expression vector and its construction method and application only need to be constructed once, and the obtained recombinant plasmid vector and transgenic plants can be used for overexpression research and subcellular localization research at the same time, and the obtained transgenic plants or tissue samples can be very convenient and fast positive test

Images