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Chip for implementing cellular localization culture based on single-cell capture and using and preparation method thereof

A cell localization and single cell technology, applied in epidermal cells/skin cells, tissue cells/virus culture devices, biochemical equipment and methods, etc., can solve problems such as single cell capture and culture chip alignment and bonding difficulties , to achieve the effects of simplified steps, high single cell rate, and simple preparation process

Inactive Publication Date: 2016-10-26
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is: in order to solve the problem of difficult alignment and bonding of existing single cell capture and culture chips, the present invention provides an integrated cell positioning culture chip based on single cell capture

Method used

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  • Chip for implementing cellular localization culture based on single-cell capture and using and preparation method thereof
  • Chip for implementing cellular localization culture based on single-cell capture and using and preparation method thereof
  • Chip for implementing cellular localization culture based on single-cell capture and using and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The chip in this example is used to culture fibroblast L929 for cell localization culture research. L929 average diameter d in suspension 0 is 10 μm.

[0029] refer to Figure 1 ~ Figure 3 . In this example, the integrated cell localization culture chip based on single cell capture was used for single L929 cell culture. Among them, the structural dimensions of the integrated cell positioning culture chip based on single cell capture are as follows: the size of the cell capture area 3 capture inlet d 1 =15μm; interception micro-column width d 2 =5μm; side length of culture area d 3 =50μm; D=70μm; the lateral distance between two adjacent structure arrays w 1 =90μm; the vertical distance between two adjacent structure arrays w 2 = 100 μm; w 3 =15 μm; etching depth h=15 μm.

[0030] In this example, the integrated cell positioning culture chip based on single cell capture is completed using MEMS technology and replication molding technology, and specifically inclu...

Embodiment 2

[0043] The chip in this example is used to culture human mammary epithelial cells HMEC for single cell capture and localized culture research. Average diameter of HMEC in suspension state d 0 = 10 μm.

[0044] refer to Figure 1 ~ Figure 3 . In this example, an integrated cell localization culture chip based on single cell capture was used for single HMEC cell culture. Among them, the structural dimensions of the integrated cell positioning culture chip based on single cell capture are as follows: the size of the cell capture area 3 intercepts the entrance d 1 =15μm; interception microcolumn d 2 = 5 μm; the reverse entrance d in the culture area 3 = 60 μm; the overall size of the culture unit D = 80 μm; the lateral distance between two adjacent structure arrays w 1 = 100μm; the vertical distance between two adjacent structure arrays w 2 = 110 μm; w 3 =15 μm; etching depth h=15 μm.

[0045] In this example, the integrated cell positioning culture chip based on single cel...

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Abstract

The invention relates to a single-cell capture and localization culture chip based on a micro-processing technology and a using and preparation method thereof and belongs to the field of bio-micro-electromechanical systems (MEMS). The chip is composed of a cellular localization culture layer and a slide layer. The cellular localization culture layer comprises a sample inlet 1, a sample outlet 8 and a plurality of cellular localization culture units between the sample inlet 1 and the sample outlet 8. When the chip is used, first cell suspension is added from the sample inlet 1, and cells enter a capture area 3 in an array; culture solution containing no cell is added from a sample outlet 7, captured cells are subjected to fluid thrust and enters a cell culture area 5 along a channel, and the cells undergo adherent culture in a culture structure. According to the chip, the contradiction between a small space needed for cell capture and a large space needed for cell culture spreading is solved, the cell capture area and the cell culture area are integrated on the chip, the cells can be captured accurately to obtain a high single cell rate, and an enough spreading space can be provided for single cells so that the cells can grow adherently.

Description

[0001] Field [0002] The invention relates to a single cell capture and positioning culture chip based on microfabrication technology and its use and preparation method, belonging to the field of biological micro-electromechanical systems (Bio-MEMS). Background technique [0003] As an experimental tool for anchorage-dependent cell research and behavior control, the single-cell localization culture chip enables people to more clearly understand the essential differences of each cell in the cell population, understand the interaction force and signal transduction between cells, and analyze How individual cells interact to form tissues and organs, and then understand various life phenomena from the single-cell level. Therefore, it has broad application prospects in the fields of cell biology, drug detection, biosensors and disease diagnosis. Traditionally, there are two schemes for using microfabrication technology to achieve cell positioning culture. One is to modify the surf...

Claims

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Application Information

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IPC IPC(8): C12M3/04C12N5/071
CPCC12M23/02C12M33/00C12N5/0631
Inventor 叶芳谢晋何美莹骞爱荣常洪龙
Owner NORTHWESTERN POLYTECHNICAL UNIV
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