37 results about "Molecular function" patented technology

The invention discloses a protein complex identification method based on knowledge enhancement graph comparative learning, and belongs to the technical field of bioinformatics. Edge trimming is performed by using a gene expression profile and subcellular localization data, so that a knowledge enhancement PPI network is constructed; and incorporating the molecular function and the biological process as network attributes as attributes for enhancing the network. Graph structure enhancement is applied to knowledge enhanced PPI attribute networks, and multiple perturbation views are generated with diverse random perturbation policies including edge addition, edge discarding, and feature masking to help mitigate data sparsity. A perturbation view generated by graph structure enhancement is coded through a randomized graph convolutional network layer, so that multi-scale features are extracted, key PPI interaction is reserved, and meanwhile prediction performance is improved. And finally, the original PPI network is weighted again, and the protein complex is identified by adopting a core-affiliated strategy.

The invention relates to the technical field of microelectronic devices, in particular to a single-molecule memristor-rectifier device based on supramolecular assembly and a preparation method thereof. According to the preparation method, the guest molecule B is covalently connected with the tail end of the graphene electrode pair, so that stable and tight interface bonding between the supramolecular functional molecule and the electrode can be ensured; and then soaking the graphene electrode pair connected with the guest molecule B in a solution containing the host molecule A and the guest molecule C to form a supramolecular functional molecule between the graphene electrode pair. The guest molecule C in the supramolecular functional molecule has intrinsic oxidation-reduction activity, and the molecular structure of the guest molecule C can generate a reversible electron gain and loss process, so that controllable and reversible adjustment of charge distribution in the molecule is realized. Under the action of an electric field, through the reversible oxidation-reduction reaction of the guest molecule C, significant changes of intramolecular charge distribution and electron transmission characteristics are triggered, and finally reliable switching of high and low resistance states of the device is achieved.

The invention discloses a preparation method and application of a peptide specific quality nanoprobe. The nanoprobe is prepared by uniformly mixing mass tag molecules, functional polypeptide and a chloroauric acid solution and adding a sodium hydroxide solution through a simple one-pot method. The synthesized mass nanoprobe is specifically recognized and combined with target protein on cells or tissues through a peptide chain, and mass-to-charge ratio signals of mass tag molecules corresponding to different nanoprobes are detected through laser desorption ionization-time-of-flight mass spectrometry (LDI-TOFMS) ionization; the mass spectrum imaging analysis on the relative expression quantity of various proteins on different types of cancer cells and tumor tissue slices is realized. The probe has the characteristics of simple preparation, specific recognition, low background, high resolution and the like, and is easy to popularize to the detection of various proteins on the surfaces of other biological samples.

In one aspect, a novel method that allows for evaluation of an RTK activity is provided. Provided is a method for evaluating an RTK activity, the method including: a step (A1) for determining diffusion dynamics A1 of the RTK by contacting a target substance with a cell that expresses a labeled RTK on a cell membrane, a step (B1) for determining diffusion dynamics B1 of the RTK by contacting the target substance and a ligand that activates the RTK with the cell that expresses the labeled RTK on a cell membrane, a step (C1) for calculating a ratio of the diffusion dynamics A1 of the RTK obtained at the step (A1) and the diffusion dynamics B1 of the RTK obtained at the step (B1), and a step (D1) for evaluating an effect of the target substance on an RTK activity by comparing the ratio of the diffusion dynamics obtained at the step (C1) with a threshold; and at the step (D1), when the ratio of the diffusion dynamics obtained at the step (C1) is equal to or greater than the threshold, it can indicate that the target substance has an effect on a molecular function of the RTK.

The invention provides a molecular interaction network protection system based on structural biology and an optimization method thereof, and relates to the technical field of biotechnology and molecular engineering.The molecular interaction network protection system comprises a USAG-1 protein binding domain, and the USAG-1 protein binding domain comprises a Glu32-Arg58-Ser76 hydrogen bond network and a Phe41 / Trp65 / Leu83 hydrophobic core; the MMP-9 cleavage site mutant is used for carrying out 20 substitutions from D to N / V / A in the PLGLA sequence, and is combined with G52S mutation; the ellipticity of the CD spectrum of the RGD peptide at 222nm is less than or equal to-15 mdeg, and the RGD peptide comprises a salt bridge at the i / i + 4 position. The USAG-1 protein binding domain also comprises an Asn45 carbohydrate chain, and the molecular weight of the Asn45 carbohydrate chain is 500 to 2000 Da. The phi angle of the RGD peptide is-57 + / -5 degrees, and the psi angle of the RGD peptide is-47 + / -5 degrees. According to the invention, through a multi-dimensional structure locking strategy, while the functional activity of biomolecules is maintained, accurate control of alpha-helical conformation is realized, a resistance mutation library is constructed, and the fundamental contradiction of stability-activity imbalance, conformation control misalignment and single-point mutation drug resistance in the traditional technology is thoroughly solved.

The application discloses a kind of molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection and a preparation method thereof, and relates to the technical field of molecular imprinting.The method comprises the preparation of pyrazolone functional group modified magnetic nanomicrospheres, dispersing the pyrazolone functional group modified magnetic nanomicrospheres, D-dimer template molecules, functional monomers and crosslinking agent in a phosphate buffer solution, uniformly mixing, and then adding an initiator to carry out a polymerization reaction.After the polymerization reaction is completed, the D-dimer template molecules are eluted and removed, and the molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection are obtained.The application utilizes the synergistic effect of salt bridge interaction and hydrogen bond interaction between arginine residues in D-dimer and pyrazolone functional groups, and successfully prepares magnetic molecularly imprinted microspheres with high recognition specificity for D-dimer.

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

A data storage medium is disclosed comprising a two-dimensional (2D) support structure onto which artificially synthesized DNA molecules encoding digital information are placed and then covered with a protective layer. The 2D support structure is formed from a material such as metal foil, glass, or plastic. The 2D support structure may be functionalized with positively charged molecules to improve DNA adhesion. The DNA is protected from degradation by encapsulation in a protective layer of a non-reactive material such as silica or a thin layer of metal. A process for storing DNA on 2D support structures is also disclosed. Correlation of specific DNA molecules with a physical storage location on a 2D support structure provides geometric addressability for selective access to specific digital information.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The invention discloses a tsRNA-SecTCA-L35 molecule and application of the tsRNA-SecTCA-L35 molecule as a colorectal cancer diagnosis biomarker and a colorectal cancer treatment target, and relates to the technical field of biological medicine. The invention provides application of the tsRNA-SecTCA-L35 molecule as a colorectal cancer diagnosis biomarker and a treatment target, in the diagnosis level, the molecule as a novel biomarker can be sensitively and specifically detected in samples such as plasma and tissue through qRT-PCR and other technologies, a brand new tool is provided for early discovery and non-invasive diagnosis of colorectal cancer, and the application of the tsRNA-SecTCA-L35 molecule as the colorectal cancer diagnosis biomarker and the treatment target is wide. In the aspect of treatment, by designing an analogue related to the molecular function and preparing the analogue into a pharmaceutical composition of a carrier such as lipidosome, the expression of the analogue in tumor cells can be effectively up-regulated, so that the proliferation of cancer cells is remarkably inhibited in vivo and in vitro, the death of the cancer cells is promoted, and the definite anti-tumor activity is shown; the important clinical application value is realized.

The invention discloses application of steroid saponin of trillium tschonoskii maxim to preparation of a medicine for treating vascular dementia, and relates to the technical field of medicines. Animal experiments prove that the berberis apiacea steroid saponin can improve the spatial reference learning function of cerebral ischemia rats, improve the spatial reference memory function of the cerebral ischemia rats and enhance the long-term memory function of the cerebral ischemia rats. In addition, brain tissue damage and white matter structure damage of rats suffering from cerebral ischemia can be relieved, axonal myelin sheath microstructure reconstruction and white matter fiber reconstruction of the rats suffering from cerebral ischemia are promoted, and myelin sheath regeneration of rats suffering from vascular dementia is promoted. Network pharmacological analysis finds that the age grass total steroid saponin has a wide regulation effect on OPCs and OLs cell components and molecular functions, and further, OPCs differentiation myelin sheath inhibition related factors are regulated and controlled, and white matter myelin sheath regeneration is promoted.

The invention discloses a circular RNA (Ribonucleic Acid) hsacirc0013599 and application of the circular RNA hsacirc0013599 in preparation of a medicine for treating cleft lip and palate, the hsacirc0013599 can participate in generation and development of CP (Clear Protein) by promoting an epithelial mesenchymal transformation process, and the hsacirc0013599 plays a molecular function through miRNA (Micro Ribonucleic Acid) and mRNA (Messenger Ribonucleic Acid) and participates in regulating the balance of proliferation and migration in a related signal channel in an HEPM (High Efficiency Particulate Matrix) embryonic development process to regulate the development of a cleft lip and palate. The circular RNA hsacirc0013599 has the advantages that the circular RNA hsacirc0013599 can be used as a marker for early screening, diagnosis and treatment of CP, the circular RNA hsacirc0013599 has a good application prospect in medicines for regulating and controlling HEPM behaviors and epithelial-mesenchymal transition, and a research basis is provided for preparing medicines for early screening of cleft lip and palate and treating cleft lip and palate.

The application discloses a kind of nanochannel devices for Fe 3+ Ion concentration detection, comprising a nanochannel for containing the electrolyte solution to be measured, the inner surface of the nanochannel is modified with functional molecules, and the functional molecules realize specific binding by chelation, coordination or electrostatic attraction with Fe 3+ Ion, so that the ion current flowing through the nanochannel changes under the applied voltage, realizing the detection of Fe 3+ Ion concentration in the electrolyte solution to be measured. The application also discloses a detection method, and the quantitative detection of Fe 3+ Ion concentration can be achieved by establishing a standard curve, which can quickly, efficiently and quantitatively detect the Fe 3+ Ion concentration in unknown water samples. Since the nanochannel can be recycled and reused, the detection cost is greatly reduced, and it can be used in water quality evaluation, health monitoring and other fields.

The present application belongs to the field of botany and genetic engineering technology, and more particularly, the present application relates to the application of rice miR5513 in the resistance of rice to brown planthopper; the present application down-regulates or knocks out the expression of miR5513 or its precursor in rice susceptible to pests based on target mimicry and CRISPR / Csa9 technology, thereby enhancing the resistance of rice to brown planthopper; compared with traditional chemical control, improving the resistance of crops through genetic engineering is a more environmentally friendly and sustainable solution. It reduces the amount and frequency of pesticide use, reduces environmental pollution and damage to the ecosystem, and the research results of the present application have reference significance for the study of gene molecular function and breeding.

The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of:(A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and(B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

The invention belongs to the technical field of circulating tumor cell detection, and provides a bionic circulating tumor cell detection device. The device comprises a detection molecule, a function construction molecule and a bionic blocking molecule, wherein the molecular structure of the bionic blocking molecule is sulfydryl-A-B; the molecular structure of the function construction molecule is sulfydryl-C-D; the molecular structure of the detection molecule is E-F-G; the A group and the C group are hydrophobic chains; the group B is a bionic amphoteric group; the D group and the E group can be subjected to reversible reaction; the F group is a hydrophilic chain; the G group is a capturing group and can capture circulating tumor cells; the functional construction molecules and the bionic blocking molecules are fixedly connected with the surface gold coating through sulfydryl. According to the device provided by the invention, a biological orthogonal reaction is carried out on the surface of the device to accurately and nondestructively capture the MGE treatment labeled CTC, the captured CTC is nondestructively released through a reversible reaction, and accurate and nondestructive detection of the CTC is realized and cyclic regeneration of the detection device is realized through a detection process of a'labeling-capturing / releasing 'device.

The invention discloses a preparation method and application of a specific membrane material based on a molecular imprinting technology, and belongs to the technical field of sewage treatment. The method realizes preparation of the membrane material through five steps: firstly, preparing a polyvinyl alcohol, polyacrylonitrile or polyvinylidene fluoride nanofiber base membrane by adopting an electrostatic spinning method, and carrying out ethanol ultrasonic cleaning and vacuum drying pretreatment; then preparing a molecular imprinting prepolymer solution containing target template molecules, functional monomers, a cross-linking agent and a solvent according to a molar ratio of 1: (3-6): (0.5-2): (20-40), and doping 1-3% of graphene quantum dots / carboxylated carbon nanotubes to enhance the performance, or optimizing the imprinting effect by adopting a two-step self-assembly method; soaking the base membrane in the pre-polymerization liquid, adding a photonic crystal precursor, and carrying out constant-temperature reaction at 30-60 DEG C for 4-8 hours to establish a surface imprinting and ordered face-centered cubic photonic crystal structure; and finally, carrying out oscillation elution and vacuum drying by using an eluent to obtain the specific membrane material. A precise recognition hole is constructed through a molecular imprinting technology, and the problem that a traditional material is poor in selectivity is solved; and the mass transfer distance is shortened by the nanofiber porous structure.

The invention relates to an RNA (Ribonucleic Acid) co-immunoprecipitation-based RNA binding protein aggregate molecular function detection method, which comprises the following steps: S1) RNA co-immunoprecipitation: firstly, splitting tissues or cells, then cleaning by using magnetic beads to remove a splitting solution, and then preparing a detection sample; s2) protein and RNA detection: firstly carrying out semi-denaturation gel electrophoresis on the sample, confirming the IP efficiency of the sample, and then recovering RNA on the membrane; s3) releasing RNA from the membrane: incubating by adopting a prepared PK buffer solution to perform protein digestion so as to extract RNA, measuring the concentration of RNA, and performing reverse transcription to obtain RNA formed by combining a DAZL polymer and a DAZL monomer; s4) performing qPCR (quantitative polymerase chain reaction) detection on a target target: performing function detection, and verifying whether RNA (ribonucleic acid) exists in the aggregate formed by combining the DAZL polymer and the monomer. The method is simple, efficient and accurate, and provides a more convenient platform for functional research of the polyamyloid protein.

The application belongs to the field of plant molecular biology and genetic engineering, and specifically discloses a negative regulation Gossypium hirsutum fruit branch length trait gene Gh_A08G254200, which is located on A08 chromosome 122390509~122395098, the gene is composed of two exons and one intron, there is a SNP site at A08:122392954, which is a non-synonymous mutation, the gene is named as GhCSLD3, the CDS sequence length is 3432 bp, the nucleotide sequence of the gene comprises SEQ ID No.1; the amino acid sequence of the encoded protein comprises SEQ ID No.2. In the extreme Gossypium hirsutum non-zero type cotton variety, the gene is silenced, and it is found that the Gossypium hirsutum fruit branch length is elongated, so the gene has a negative regulation effect on the Gossypium hirsutum fruit branch elongation. The verification of the molecular function of the gene has important significance for improving the breeding of Gossypium hirsutum plant type change.

The application discloses a new application of m6A modified gene GAS6 and its receptor MERTK in rheumatoid arthritis. Multi-omics integrated analysis of synovial tissue and peripheral blood mononuclear cells of RA patients reveals common dysregulation of transcriptomics and epitranscriptomics, highlighting genes with both differential expression and m6A modification, which are enriched in processes such as phagocytosis, Th17 differentiation and cell aging. Among these genes, GAS6 shows the most significant m6A hypermethylation and expression up-regulation, and is verified as a key effector molecule interacting with MERTK / AXL receptor. Functional experiments show that GAS6 and MERTK synergistically promote the malignant phenotype of RA fibroblast-like synoviocytes, enhance their proliferation, migration, inflammatory cytokine secretion and anti-apoptotic ability.

The application discloses a nanopore detection probe connected with a target molecule to be detected and a support molecule, and a preparation method and application thereof. The nanopore detection probe is obtained by coupling the target molecule to be detected and the support molecule through a bifunctional cross-linking reagent; wherein the support molecule is composed of a guest interaction molecule, a functional connecting group and a pulling sequence; the guest interaction molecule provides a specific signal for the nanopore generated in a combined detection system of host interaction molecules in a solution; the functional connecting group provides an action site of the bifunctional cross-linking reagent, so as to be combined with the target molecule to be detected; and the pulling sequence provides power for driving the whole probe molecule to enter a nanopore detection area under the action of an electrophoretic force. The target molecule to be detected is detected by using the nanopore detection probe and a nanopore single-molecule technology, so that characteristic signals are generated to perform qualitative and / or quantitative analysis. Taking an amino acid-support molecule as an example, twenty different amino acids can be distinguished by the method.

Aspects of the disclosure relate to methods for determining whether or a subject is likely to respond to certain adoptive cell therapies (e.g., chimeric antigen receptor (CAR) T-cell therapy, etc.). In some embodiments, the methods comprise the steps of identifying a subject as having a tumor microenvironment (TME) type based upon a molecular-functional (MF) expression signature of the subject, and determining whether or not the subject is likely to respond to a chimeric antigen receptor (CAR) T-cell therapy based upon the TME type. In some embodiments, the methods comprise determining the lymphoma microenvironment (LME) type of a lymphoma (e.g., Diffuse Large B cell lymphoma (DLBCL)) subject and identifying the subjects prognosis based upon the LME type determination.

The invention belongs to the technical field of gene engineering and plant cultivation, and particularly relates to a BroMYB34 gene synthesized by inducing kale flavonoid through selenium and application of the BroMYB34 gene. Specifically, the selenium-induced BroMYB34 gene in the kale is identified and cloned for the first time, the molecular function of the selenium-induced BroMYB34 gene serving as a transcription factor for regulating and controlling flavonoid synthesis is clarified, and the BroMYB34 gene is over-expressed, so that the flavonoid content of the kale can be remarkably increased, the oxidation resistance of the kale can be enhanced, and the application prospect is broad. An efficient and oriented genetic engineering technical approach is provided for the quality improvement of the kale; meanwhile, the BroMYB34 gene has potential function conservative property in cruciferous plants, can be expanded and applied to quality improvement of various vegetables, and has important significance in promoting breeding and industrialization of functional vegetables, so that the BroMYB34 gene has good practical application value.

The invention provides a method for identifying long-chain non-coding RNA (Ribonucleic Acid) interacting protein, and relates to the technical field of biology. The method comprises the following steps: immobilizing RNA-protein interaction in cells through ultraviolet crosslinking, hybridizing with a biotin-modified probe after cracking the cells, capturing a probe-RNA-protein compound by using streptavidin magnetic beads, eluting by using an eluent containing lauryl sodium sulfate with a specific concentration, and finally carrying out protein precipitation and mass spectrometry. The identification of the target long-chain non-coding RNA interaction protein is realized. The method is easy and convenient to operate, high in specificity and suitable for tissue samples, protein interacting with specific long-chain non-coding RNA can be efficiently and accurately recognized, and a powerful tool is provided for studying the molecular function of the long-chain non-coding RNA.

This invention relates to the field of microelectronic device technology, and more particularly to a single-molecule memristor-rectifier device based on supramolecular assembly and its fabrication method. The fabrication method involves covalently linking a guest molecule B to the ends of a graphene electrode pair, ensuring a stable and tight interfacial bond between the supramolecular functional molecule and the electrode. Subsequently, the graphene electrode pair connected to the guest molecule B is immersed in a solution containing a host molecule A and a guest molecule C, forming a supramolecular functional molecule between the graphene electrode pairs. The guest molecule C in the supramolecular functional molecule possesses intrinsic redox activity; its molecular structure itself can undergo reversible electron gain and loss processes, thereby achieving controllable and reversible adjustment of the intramolecular charge distribution. Under the influence of an electric field, the reversible redox reaction of the guest molecule C triggers significant changes in the intramolecular charge distribution and electron transport characteristics, ultimately achieving reliable switching between high and low resistance states of the device.

Provided is an engineered cell expressing truly epitope-receptive MHC ectodomains, comprising a human leukocyte antigen molecule (HLA molecule), wherein the HLA molecule comprises a first chain and a second chain, wherein the first chain of the HLA molecule is selected from HLA-I heavy chains or HLA-II alpha chains, the second chain of the HLA molecule is selected from HLA-I light chains or HLA-II beta chains, and the first and second chains of the HLA molecule are non-covalently linked to form an HLA molecule with native HLA molecular functions. The engineered cells can be used for rapid identification and characterization of MHC restricted antigen peptides, and can efficiently and accurately develop and validate HLA neoantigen targeting immunotherapeutic biologics and drug candidates.

Disclosed herein is a microfluidic device for priming, collection and recovery of immune cells comprising: a) two separate fluidic channels: i) a first channel, wherein the first channel is infused with immune cells, and ii) a second channel, wherein the second channel acts as a collection reservoir for transmigrated cells, and; b) a microporous membrane interface, present between the first channel and the second channel, wherein the microporous membrane is functionalized on the first channel surface with adhesion molecules. Also disclosed are methods of creating enhanced cells, and using them in treatment of individuals in need thereof.

This invention discloses a multimodal cervical cancer immune subtype prediction method based on deep learning, including the following steps: (1) Data acquisition: acquiring pathological whole slide image data and transcriptome sequencing data of cervical cancer patients; (2) Data preprocessing: performing tissue segmentation, multi-scale block division and normalization processing on the whole slide images, and performing protein-coding gene screening and standardization processing on the transcriptome data; (3) Model construction: constructing an image feature extraction branch based on attention mechanism convolutional neural network, a gene feature extraction branch based on deep fully connected network, and a multimodal fusion module based on feature splicing or decision weighting; (4) Model training: using weighted cross-entropy loss function and cosine annealing strategy to train the model and optimize parameters to solve the problem of sample class imbalance; (5) Model prediction: this invention effectively overcomes the information bottleneck of single-modal data by synergistically fusing the spatial morphological features of pathological images and the molecular functional features of gene expression, and significantly improves the accuracy and robustness of cervical cancer C1 / C2 immune subtype prediction.

The application discloses a plasma exosome ncRNA biomarker of recurrent depression and application thereof, and the biomarker comprises miR-618 and / or miR-223-3p. It is verified by RT-qPCR that the average expression levels of miR-618 and miR-223-3p of patients with recurrent depression are significantly higher than those of healthy controls, ROC curve analysis shows that miR-618 and miR-223-3p both show good diagnostic performance, both have the potential of being biomarkers of recurrent depression, and have good application prospect. In addition, the molecular function of miR-618 is verified through in-vivo experiments, and the result shows that miR-618 regulates PI3K-Akt and other signal pathways, and then causes depressive behaviors.

The invention relates to the field of bioinformatics, and discloses a method for predicting physiological age by using immune repertoire and transcriptome data, which comprises the following steps: data acquisition: acquiring clinical information, peripheral blood immune repertoire sequencing data and transcriptome sequencing data of a sample to be detected; feature extraction: preprocessing peripheral blood immune repertoire sequencing data and transcriptome sequencing data respectively, and extracting immune repertoire features and transcriptome features; feature integration: integrating the immune group library features and transcriptome features into a multi-modal feature matrix; and model prediction based on the multi-modal feature matrix and clinical information. According to the method, immune group library features and transcriptome features are integrated, collaborative analysis and feature fusion of multi-dimensional senescence signals are achieved, a to-be-detected sample is predicted through a physiological age prediction model, therefore, interaction association between immune senescence clone structure features and transcriptional regulation molecular function features is captured, and then prediction precision is improved.