90 results about "Molecular function" patented technology

An electrochemical method for determining immunity of tumor marker includes forming microreaction cell, placing reference and counter electrode above it and fixing antigen molecular functioning film at pool bottom, connecting enzyme labelled antibody to pool bottom at time of only proper quantity of horseradish peroxidase to label tumor marker antibody, obtaining cataltyic current on working electrode by contacting free immune matter with electronic media and having positive ratio of the current to antigen. The microvolume immune determining chip is also disclosed.

Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

The invention relates to a molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residues, belonging to the technical field of the detection of the pesticide residues. Theinvention also relates to a method for detecting the trace amount pesticide residues contained in agricultural product samples by using the molecular imprinting chemiluminescence sensor, which comprises the following steps: (1) selecting a functional monomer; (2) uniformly mixing a template molecule, the functional monomer, a cross linker, a pore-forming agent, an initiating agent and a silicon source to prepare an MIPs collosol according to a mole ratio of 0.1-1 to 1-10 to 5-25 to 20-60 to 0.05-0.15 to 20-50; (3) preparing a quantum dot material solution; and (4) modifying the MIPs collosoland quantum dot materials to the surface of a millipore plate to manufacture the chemiluminescence sensor by a layer-layer self-assembly surface modification technique. The invention has the advantages of high specificity and sensitivity, fast detection, low cost, good repeatability and convenient field detection on the detection of the pesticide residues.

The invention provides a preparation method and use of a graphene oxide surface molecularly imprinted sol-gel polymer and relates to the technical field of combination of polymer material science and chemical sensors. Graphene oxide as a carrier and siloxane as a functional monomer and a cross-linking agent are subjected to sol-gel method treatment in an aqueous solution at a normal temperature so that the graphene oxide surface molecularly imprinted sol-gel polymer is obtained, and the graphene oxide surface molecularly imprinted sol-gel polymer can be used in the field of electrochemical sensors and can realize fast analysis detection of a template molecule. The preparation method has the simple process of mixing the carrier, the template molecule, the functional monomer, the cross-linking agent and the solvent according to a certain ratio, has a low cost, saves energy and is environmentally friendly. The graphene oxide surface molecularly imprinted sol-gel polymer-based electrochemical sensor has the advantages of rapid response to the imprinted molecule, good selectivity, high sensitivity and good stability.

A method of generating a molecule-function network including bio-events by carrying out a connect search using a biomolecule-linkage database including information on the bio-events, and a method of predicting a pathway between an arbitrary biomolecule and an arbitrary bio-event in said network or a method of predicting the bio-events to which an arbitrary biomolecule in said network is related.

The invention discloses a preparation method of a nylon-coated high-molecular polymer powder material. The preparation method of the nylon-coated high-molecular polymer powder material comprises the following steps: firstly heating a mixture of nylon resin, an ethyl alcohol solvent, high-molecular polymer powder and an antioxidant in a sealed container, dissolving nylon in the solvent, then gradually cooling, gradually crystallizing the nylon resin by taking high-molecular polymer particles as the core, coating the surfaces of the high-molecular polymer particles with the nylon resin, carrying out vacuum drying and ball milling, and screening, so that powder with certain particle diameter distribution is selected as the nylon-coated high-molecular polymer powder material. The high-molecular polymer powder/nylon resin composite material prepared by the method is excellent in sintering furnace and high in fluidity; the material has a small amount of nylon; a selective laser sintering (SLS) initial shape blank has relatively high accuracy and strength, so that the material is very suitable for indirectly manufacturing high-molecular function parts by virtue of SLS.

The invention discloses a preparation method of a CdTe@SiO2 quantum dot surface monoamine neurotransmitter molecularly imprinted polymer. The preparation method comprises the following steps of: synthesizing CdTe@SiO2 quantum dots in a water phase, carrying out rotary evaporation till water is completely volatized, and washing off unreacted substances by absolute ethyl alcohol; adding the obtained CdTe@SiO2 quantum dots to a pore-foaming agent, sequentially adding template molecules, functional monomers and a crosslinking agent after uniform ultrasonic dispersion, and stirring and reacting for 20 hours under the protection of N2; centrifuging a reaction liquid, discarding a supernatant, and washing obtained solids to remove the template molecules to obtain the CdTe@SiO2 quantum dot surface monoamine neurotransmitter molecularly imprinted polymer. The material synthesized by the method has the advantages that a quantum dot fluorescent probe is high in sensitivity and simple and convenient to detect and has the characteristics that the molecularly imprinted polymer is high in selectivity and affinity and can be directly used for determining monoamine neurotransmitters in biological fluids.

An apparatus is provided for performing an chemical, biochemical, or biological assay on a sample comprising: a microfluidic assay cartridge (1) that contains at least one sample inlet well (2) configured to receive a sample; and a microfluidic sub-unit (3) associated with the microfluidic assay cartridge (1) and comprising microfluidic channels (8), micro-valves (4, 4a, 9) and at least one separate and fluidicly-isolated isolation channel (5), and at least one hollow element (14); the at least one hollow element (14) being functionalized with a capture moiety or molecules (15) so as to form at least one reaction vessel (19); the microfluidic channels (8) and micro-valves (4, 4a, 9) configured to respond to signaling containing information about performing the assay and to controllably receive the sample and at least one reagent in the at least one reaction vessel (19), and to provide from the at least one reaction vessel (19) light containing information about the assay performed on the sample inside the at least one reaction vessel (19) as a result of said at least one reagent.

The invention discloses a method for establishing a pancreatic cancer miRNA prognosis model and screening a targeted gene. The model comprises hsa-mir-424, hsa-mir-126, hsa-mir-3613 and hsa-mir-4772,nine key genes are identified, and the nine key genes comprise MMP14, ITGA2, THBS2, COL1A1, COL3A1, COL11A1, COL6A3, COL12A1 and COL5A2. According to the method for establishing the pancreatic cancermiRNA prognosis model, by means of TCGA and GEO databases, data is subjected to multi-step analysis through a plurality of R language installation packages and combines with clinical information, a Cox proportional risk regression model is established to search for prognosis biomarkers, the targeted gene of miRNA is predicted, the key genes related to pancreatic cancer are found out by utilizing Cytoscape, and related molecular functions and action mechanisms of the key genes are predicted through KEGG and GO analysis so as to search for new treatment targets and prognosis markers of pancreatic cancer patients.

The invention discloses a prognosis marker for the lung cancer and application thereof. The long chain non-coding RNA gene RP11-434D9.1 of the marker is quite related to the prognosis period of patients with the lung cancer, through the marker and expression for detecting the marker, lung cancer prognosis is rapidly, accurately and conveniently predicted, the grading and layering of lung cancer risks are realized according to molecular levels, and patients with different grades of risks are separated obviously. Through co-expression analysis, 238 genes related to the long chain non-coding RP1-434D9.1 are found out, through an enrichment analysis on GO and KEGG pathways of the 238 genes, the molecular functions of the long-chain non-coding RP1-434D9.1 are found out, and important biologicalexperiment clues are provided for improving prognosis molecular diagnosis of the patients with the lung cancer and related medicine targets.

The invention discloses an application of taking oligopeptide and derivatives thereof as a template molecule, and an imprinting technology based preparation method of an angiotensin synthetic receptor. The template molecule comprises any section of a peptide bond at a nitrogen end or a carbon end of an angiotensin II, and derivatives thereof, or any section of the peptide bond at the nitrogen end or the carbon end of an angiotensin I and the derivatives thereof. The application takes the template molecule as an imprint to prepare the angiotensin synthetic receptor; the angiotensin acceptor is prepared in a way that the template molecule, a function monomer, a cross-linking agent, a surface-active agent and the like are mixed according to a certain rate, and then an initiator is added. The acceptor is good in biocompatibility, can high selectively capture and neutralize the angiotensin II and the angiotensin I, and cuts off functions of the angiotensin II and the angiotensin acceptor (AT1) as well as functions of the angiotensin I and angiotensin converzyme (ACE), so as to control the blood pressure. The acceptor can be applied to separation, concentration and measuring of the angiotensin I or II.

The invention relates to an algal toxin molecular imprinting chemoreceptor sensor. The sensor comprises a three-electrode system, wherein a glassy carbon electrode is a work electrode, a platinum wire electrode is an auxiliary electrode, and Ag/AgCl is a reference electrode; the glassy carbon electrode is modified with an algal toxin molecular imprinting film which comprises algal toxin mixed template modules, function monomers, crosslinking agents and photoinitiators in the mole ratio of 1: (20-50): (10-20): (0.5-1.5). The invention further relates to a preparation method of the sensor and an application of the sensor for analysis and detection of content of algal toxin in water. The sensor is used for analysis and detection of the algal toxin in the water, operation is convenient and fast, the flexibility is high, the operation cost is low, and the sensor is easy to control and automatic, can be used for quick screening of algal toxin in the water and has high use value in fields of environment monitoring and safety control of drinking water.

The invention relates to molecular imprinting material as well as preparation method and application thereof. The method comprises the following steps of: adding a template molecule, a functional monomer and a cross-linking agent into a container according to a proportion and then standing; adding a dispersing agent; finally adding an initiating agent and a hole-inducing agent into the container according to a proportion, controlling the temperature to react and obtaining a microparticle with the particle diameter ranging between 20 microns and 350 microns; refluxing and washing the obtained microparticle by a mixed solution of methanol/acetic acid in the container and detecting by GC (gas chromatography) till triazolam is not detected; washing by distilled water to neutrality; and drying in vacuum to constant weight to obtain the microparticle which is the needed triazolam molecular imprinting material. The prepared imprinting material can be directly used for enriching and separating the triazolam in urine, reduces the dosage of an organic solvent used when the triazolam is extracted by a liquid-liquid extracting method and is beneficial to the body health of an operator. The method does not need the protection of an inert gas, and the compounded material has the characteristics of small particle diameter and uniform distribution.

The invention provides a preparation method of a halofuginone molecularly-imprinted solid-phase extraction small column and an application and relates to the field of analytical chemistry. The preparation method comprises the following steps of: taking halofuginone as a template molecule, dissolving the template molecule into a porogen and adding a functional monomer at a low temperature for prepolymerization; then, mixing according to the molar ratio of the template molecule: the functional monomer: a cross-linking agent of 1:3-6:15-30, and preparing molecularly-imprinted polymer micro-spheres in a manner of initiated polymerization at a low temperate by adopting a suspension polymerization method and filling the rinsed, sieved, eluted and dried micro-spheres in an empty column tube of the solid-phase extraction column by adopting a wet process; and later, using the micro-spheres rinsed by acetone and methanol in sequence into selective adsorption, enrichment and purification of the halofuginone remained in animal derived foods. Compared with the existing solid-phase extraction column, the halofuginone molecularly-imprinted solid-phase extraction column prepared by the invention has the characteristics of strong specificity, convenience and simplicity in preparation, good recognition performance, low cost, easiness for realization of required instruments and reaction conditions and the like.

The present invention discloses a sunset yellow molecular imprinting detection electrochemical sensor preparation method. In the method, magnetic graphene is used as a carrier, the surface is modified with 1-butyl-3-methyl imidazole hydrobromide ionic liquid and nano Au, and a sunset yellow molecular imprinting polymer is prepared by addition of a sunset yellow template molecule, a functional monomer and a crosslinking agent on the basis of the polymer. The glassy carbon electrode surface is modified with the sunset yellow molecular imprinting polymer for construction of a sunset yellow molecular imprinting detection electrochemical sensor. The electrochemical sensor constructed on the basis of the imprinted material has high sunset yellow detection selectivity and sensitivity, and can achieve rapid and sensitive detection of a real sample.

The invention relates to an el-b3a gene in a soybean growth period and a coding protein thereof. The gene sequence of the el-b3a gene in the soybean growth period is shown as a sequence list Seq ID No:1, and the amino acid sequence of the coding protein of the el-b3a gene in the soybean growth period is shown as a sequence list Seq ID No:2. By means of relevant heredity and molecular functional verification of varieties, the el-b3a gene has a flowering promoting function as compared with El genes.

The embodiment of the invention relates to application of a mycotoxin biodegradation agent to promotion of healthy pig production, in particular to application of the mycotoxin biodegradation agent tothe preparation of feed for positively regulating a microbial flora in contents of pig jejunum, improving the detoxification ability of pigs and promoting healthy growth. The mycotoxin biodegradationagent can up-regulate the expression of 153 proteins in the liver of piglets and down-regulate the expression of 55 proteins. The differential proteins are mainly involved in the composition of cellcomponents, molecular functions and biological processes. Analysis of KEGG pathways of the differential proteins shows that the mycotoxin biodegradation agent can up-regulate the expression of 13 proteins without down-regulated proteins, the KEGG pathways in which the up-regulated proteins mainly participate include endoplasmic reticulum protein processing, glycolysis/gluconeogenesis, tryptophan metabolism, valine, leucine and isoleucine metabolism, microbial metabolism in different environments, starch and sucrose metabolism and the like.

The invention relates to a preparation method and application of phloroglucinol molecularly imprinted polymers in plants. The method comprises the following steps of: mixing template molecules, a functional monomer and a cross-linking agent in a molar ratio of 1:(3-6):(15-30), and adding benzoyl peroxide and N,N-dimethylaniline which serve as redox initiators; dripping the mixture into a 3 to 8 percent polyvinyl alcohol 400 aqueous solution with stirring at the temperature of between 10 and 30 DEG C to obtain polymer microspheres; bleaching the polymer microspheres, sieving, eluting, drying and the like, and filling in a glass chromatographic column by a wet method; and moistening the polymer microspheres by using methanol and acetone sequentially to obtain a molecularly imprinted chromatographic column, so that the molecularly imprinted chromatographic column is used for the preferential adsorption and efficient enrichment of phloroglucinol substances such as hyperforin, adhyperforinand the like. Compared with the conventional chromatographic column, the prepared chromatographic column has the characteristics that the column is high in specificity, easy and convenient to prepareand low in cost, required instruments and reaction conditions are easy to implement, and the like.

This invention provides antigen substance inductors which produce highly selective and/or specific vaccine precursor, vaccine, antibody (including idiotype antibody), neutralizing antibody, antitoxin. This invention is to produce and/or manufacture highly selective and/or specific vaccine precursor, vaccine, antibody (including idiotype antibody), neutralizing antibody, antitoxin by quantum thermodynamic and chemical control of molecular functions and morphogenesis, inducing non-functional complex macromolecules which form organism and/or non-organism and which become to be substance with fundamental structure more closed to an induction of the functions, utilizing the fundamental structure of molecule which is indicated in Formula 3-a as a representative molecule. Moreover, by those produced substances, this invention is to produce and/or manufacture antimicrobial agent, antiviral agent, neutralizing antibody, antitoxin, antitumor agent, anti-protozoa agent (malaria, spirochaeta et. al), molecular discriminating agent, antibody as labeled compounds, histocompatible accelerator on tissues or organs, immuno-response accelerator or immuno-response controller, complement chain reaction accelerator.