56 results about "Molecular function" patented technology

The invention discloses a protein complex identification method based on knowledge enhancement graph comparative learning, and belongs to the technical field of bioinformatics. Edge trimming is performed by using a gene expression profile and subcellular localization data, so that a knowledge enhancement PPI network is constructed; and incorporating the molecular function and the biological process as network attributes as attributes for enhancing the network. Graph structure enhancement is applied to knowledge enhanced PPI attribute networks, and multiple perturbation views are generated with diverse random perturbation policies including edge addition, edge discarding, and feature masking to help mitigate data sparsity. A perturbation view generated by graph structure enhancement is coded through a randomized graph convolutional network layer, so that multi-scale features are extracted, key PPI interaction is reserved, and meanwhile prediction performance is improved. And finally, the original PPI network is weighted again, and the protein complex is identified by adopting a core-affiliated strategy.

The invention relates to the technical field of microelectronic devices, in particular to a single-molecule memristor-rectifier device based on supramolecular assembly and a preparation method thereof. According to the preparation method, the guest molecule B is covalently connected with the tail end of the graphene electrode pair, so that stable and tight interface bonding between the supramolecular functional molecule and the electrode can be ensured; and then soaking the graphene electrode pair connected with the guest molecule B in a solution containing the host molecule A and the guest molecule C to form a supramolecular functional molecule between the graphene electrode pair. The guest molecule C in the supramolecular functional molecule has intrinsic oxidation-reduction activity, and the molecular structure of the guest molecule C can generate a reversible electron gain and loss process, so that controllable and reversible adjustment of charge distribution in the molecule is realized. Under the action of an electric field, through the reversible oxidation-reduction reaction of the guest molecule C, significant changes of intramolecular charge distribution and electron transmission characteristics are triggered, and finally reliable switching of high and low resistance states of the device is achieved.

The present invention belongs to the field of plant genetic engineering technology, and in particular to a rice cytokinin response regulator gene OsRR24 And application, the rice cytokinin response regulatory factor gene OsRR24 It encodes a protein containing a DNA binding domain and a conserved aspartate-containing signal receiving domain, and is a response regulator downstream of the rice CK signaling pathway. OsRR24 During the functional process in rice, it was transferred into Nipponbare and Bph6 In the NIL, overexpressing plants showed significantly enhanced resistance to brown planthoppers, while RNAi-inhibited plants showed significantly reduced resistance to brown planthoppers. The gene of this invention provides a good theoretical basis for studying how cytokinins participate in rice insect resistance through response regulatory factors. It has reference significance for studying gene molecular functions and also provides genetic resources for the design and breeding of insect-resistant rice varieties.

The invention discloses a preparation method and application of a peptide specific quality nanoprobe. The nanoprobe is prepared by uniformly mixing mass tag molecules, functional polypeptide and a chloroauric acid solution and adding a sodium hydroxide solution through a simple one-pot method. The synthesized mass nanoprobe is specifically recognized and combined with target protein on cells or tissues through a peptide chain, and mass-to-charge ratio signals of mass tag molecules corresponding to different nanoprobes are detected through laser desorption ionization-time-of-flight mass spectrometry (LDI-TOFMS) ionization; the mass spectrum imaging analysis on the relative expression quantity of various proteins on different types of cancer cells and tumor tissue slices is realized. The probe has the characteristics of simple preparation, specific recognition, low background, high resolution and the like, and is easy to popularize to the detection of various proteins on the surfaces of other biological samples.

In one aspect, a novel method that allows for evaluation of an RTK activity is provided. Provided is a method for evaluating an RTK activity, the method including: a step (A1) for determining diffusion dynamics A1 of the RTK by contacting a target substance with a cell that expresses a labeled RTK on a cell membrane, a step (B1) for determining diffusion dynamics B1 of the RTK by contacting the target substance and a ligand that activates the RTK with the cell that expresses the labeled RTK on a cell membrane, a step (C1) for calculating a ratio of the diffusion dynamics A1 of the RTK obtained at the step (A1) and the diffusion dynamics B1 of the RTK obtained at the step (B1), and a step (D1) for evaluating an effect of the target substance on an RTK activity by comparing the ratio of the diffusion dynamics obtained at the step (C1) with a threshold; and at the step (D1), when the ratio of the diffusion dynamics obtained at the step (C1) is equal to or greater than the threshold, it can indicate that the target substance has an effect on a molecular function of the RTK.

The invention provides a molecular interaction network protection system based on structural biology and an optimization method thereof, and relates to the technical field of biotechnology and molecular engineering.The molecular interaction network protection system comprises a USAG-1 protein binding domain, and the USAG-1 protein binding domain comprises a Glu32-Arg58-Ser76 hydrogen bond network and a Phe41 / Trp65 / Leu83 hydrophobic core; the MMP-9 cleavage site mutant is used for carrying out 20 substitutions from D to N / V / A in the PLGLA sequence, and is combined with G52S mutation; the ellipticity of the CD spectrum of the RGD peptide at 222nm is less than or equal to-15 mdeg, and the RGD peptide comprises a salt bridge at the i / i + 4 position. The USAG-1 protein binding domain also comprises an Asn45 carbohydrate chain, and the molecular weight of the Asn45 carbohydrate chain is 500 to 2000 Da. The phi angle of the RGD peptide is-57 + / -5 degrees, and the psi angle of the RGD peptide is-47 + / -5 degrees. According to the invention, through a multi-dimensional structure locking strategy, while the functional activity of biomolecules is maintained, accurate control of alpha-helical conformation is realized, a resistance mutation library is constructed, and the fundamental contradiction of stability-activity imbalance, conformation control misalignment and single-point mutation drug resistance in the traditional technology is thoroughly solved.

Provided herein are methods of functionalizing C(sp3)-H bonds using reprogramed metalloenzymes to perform radical-relay C(sp3)-H functionalization, activating a (sp3)-H bond via a reactive radical (X·) via hydrogen atom transfer (HAT); intercepting of the resulting carbon-centered radical by a redox-reactive metal complex; and obtaining a functionalized C—Y bond.

The application discloses a kind of molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection and a preparation method thereof, and relates to the technical field of molecular imprinting.The method comprises the preparation of pyrazolone functional group modified magnetic nanomicrospheres, dispersing the pyrazolone functional group modified magnetic nanomicrospheres, D-dimer template molecules, functional monomers and crosslinking agent in a phosphate buffer solution, uniformly mixing, and then adding an initiator to carry out a polymerization reaction.After the polymerization reaction is completed, the D-dimer template molecules are eluted and removed, and the molecularly imprinted magnetic microspheres for D-dimer high-efficiency detection are obtained.The application utilizes the synergistic effect of salt bridge interaction and hydrogen bond interaction between arginine residues in D-dimer and pyrazolone functional groups, and successfully prepares magnetic molecularly imprinted microspheres with high recognition specificity for D-dimer.

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

A data storage medium is disclosed comprising a two-dimensional (2D) support structure onto which artificially synthesized DNA molecules encoding digital information are placed and then covered with a protective layer. The 2D support structure is formed from a material such as metal foil, glass, or plastic. The 2D support structure may be functionalized with positively charged molecules to improve DNA adhesion. The DNA is protected from degradation by encapsulation in a protective layer of a non-reactive material such as silica or a thin layer of metal. A process for storing DNA on 2D support structures is also disclosed. Correlation of specific DNA molecules with a physical storage location on a 2D support structure provides geometric addressability for selective access to specific digital information.

The invention discloses internal peroxide singlet oxygen carrier molecules capable of being quickly converted and application thereof, and belongs to the field of functional molecule design and intelligent materials. The molecules are functionalized on the ferroferric oxide magnetic nano material, and a singlet oxygen storage system which is kept inert under the normal temperature condition is successfully constructed. When the system is in contact with fluorine ion sources such as tetrabutylammonium fluoride and the like, carbon-silicon bonds are triggered to break through nucleophilic substitution reaction, so that a molecular structure is promoted to be quickly converted into 1, 4-dimethylnaphthalene endoperoxide and is subjected to ring cracking, singlet oxygen is released, and accurate regulation and control of a molecular switch are realized. A DPBF probe verifies that singlet oxygen release of the system can be controlled through fluorine ions, and excellent time-space controllability is shown. Furthermore, a magnetically-controlled singlet oxygen source is constructed by loading on a ferroferric oxide nano-carrier, and a singlet oxygen nano-carrier controlled by a magnetic field is constructed; the limitation that a traditional photosensitizer depends on an external light source and oxygen is broken through, and wide application prospects are achieved.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The invention discloses a tsRNA-SecTCA-L35 molecule and application of the tsRNA-SecTCA-L35 molecule as a colorectal cancer diagnosis biomarker and a colorectal cancer treatment target, and relates to the technical field of biological medicine. The invention provides application of the tsRNA-SecTCA-L35 molecule as a colorectal cancer diagnosis biomarker and a treatment target, in the diagnosis level, the molecule as a novel biomarker can be sensitively and specifically detected in samples such as plasma and tissue through qRT-PCR and other technologies, a brand new tool is provided for early discovery and non-invasive diagnosis of colorectal cancer, and the application of the tsRNA-SecTCA-L35 molecule as the colorectal cancer diagnosis biomarker and the treatment target is wide. In the aspect of treatment, by designing an analogue related to the molecular function and preparing the analogue into a pharmaceutical composition of a carrier such as lipidosome, the expression of the analogue in tumor cells can be effectively up-regulated, so that the proliferation of cancer cells is remarkably inhibited in vivo and in vitro, the death of the cancer cells is promoted, and the definite anti-tumor activity is shown; the important clinical application value is realized.

The invention provides a method for predicting the influence of graphene quantum dots on biological organisms based on transcriptome sequencing, and belongs to the technical field of gene transcriptomics. The invention adopts a high-throughput transcriptome sequencing technology to analyze the influence of 10 [mu] g / ml GOQD on the mouse bone marrow MSC transcriptome level, and reveals the potential results of the biological process caused by the stimulation of 10 [mu] g / ml GOQD on mouse bone marrow MSC, molecular function and molecular biology signal pathway. The invention further discloses the influence of 10mu g / ml GOQD on the immune function, biological development and cell division process and cardiovascular generation and growth process of mice and an action target gene of the GOQD. The hub protein coded by the acting target gene is screened from the perspective of proteomics, and a detection index is provided for monitoring the influence of GOQD on organisms.

The invention discloses application of steroid saponin of trillium tschonoskii maxim to preparation of a medicine for treating vascular dementia, and relates to the technical field of medicines. Animal experiments prove that the berberis apiacea steroid saponin can improve the spatial reference learning function of cerebral ischemia rats, improve the spatial reference memory function of the cerebral ischemia rats and enhance the long-term memory function of the cerebral ischemia rats. In addition, brain tissue damage and white matter structure damage of rats suffering from cerebral ischemia can be relieved, axonal myelin sheath microstructure reconstruction and white matter fiber reconstruction of the rats suffering from cerebral ischemia are promoted, and myelin sheath regeneration of rats suffering from vascular dementia is promoted. Network pharmacological analysis finds that the age grass total steroid saponin has a wide regulation effect on OPCs and OLs cell components and molecular functions, and further, OPCs differentiation myelin sheath inhibition related factors are regulated and controlled, and white matter myelin sheath regeneration is promoted.

The invention discloses a circular RNA (Ribonucleic Acid) hsacirc0013599 and application of the circular RNA hsacirc0013599 in preparation of a medicine for treating cleft lip and palate, the hsacirc0013599 can participate in generation and development of CP (Clear Protein) by promoting an epithelial mesenchymal transformation process, and the hsacirc0013599 plays a molecular function through miRNA (Micro Ribonucleic Acid) and mRNA (Messenger Ribonucleic Acid) and participates in regulating the balance of proliferation and migration in a related signal channel in an HEPM (High Efficiency Particulate Matrix) embryonic development process to regulate the development of a cleft lip and palate. The circular RNA hsacirc0013599 has the advantages that the circular RNA hsacirc0013599 can be used as a marker for early screening, diagnosis and treatment of CP, the circular RNA hsacirc0013599 has a good application prospect in medicines for regulating and controlling HEPM behaviors and epithelial-mesenchymal transition, and a research basis is provided for preparing medicines for early screening of cleft lip and palate and treating cleft lip and palate.

The application discloses a kind of nanochannel devices for Fe 3+ Ion concentration detection, comprising a nanochannel for containing the electrolyte solution to be measured, the inner surface of the nanochannel is modified with functional molecules, and the functional molecules realize specific binding by chelation, coordination or electrostatic attraction with Fe 3+ Ion, so that the ion current flowing through the nanochannel changes under the applied voltage, realizing the detection of Fe 3+ Ion concentration in the electrolyte solution to be measured. The application also discloses a detection method, and the quantitative detection of Fe 3+ Ion concentration can be achieved by establishing a standard curve, which can quickly, efficiently and quantitatively detect the Fe 3+ Ion concentration in unknown water samples. Since the nanochannel can be recycled and reused, the detection cost is greatly reduced, and it can be used in water quality evaluation, health monitoring and other fields.

The application provides a tobacco target spot pathogen pathogenic factor RsDN3377, a recombinant plasmid, a bacterial body and a preparation method and application of the bacterial body, and belongs to the technical field of biotechnology.The application screens a pathogenic factor generated by tobacco target spot pathogen infection of a host through an RNA-seq method, and reveals important biological functions of the RsDN3377 protein in pathogenicity of target spot pathogen and host interaction.Based on an agrobacterium infiltration transient expression method, a candidate full-length cDNA is constructed into a recombinant plasmid and a recombinant bacterial body, the recombinant bacterial body is injected into a leaf of a benhamiana tobacco N.benthamiana, and necrosis symptoms of a bacterial infection part are observed to identify a molecular function of the RsDN3377 protein.The results show that the agrobacterium infiltration transient expression protein carrying the gene RsDN3377 can cause the occurrence of cell death symptoms, and provides a new target for development and prevention and control of a tobacco target spot pathogen.

The present application belongs to the field of botany and genetic engineering technology, and more particularly, the present application relates to the application of rice miR5513 in the resistance of rice to brown planthopper; the present application down-regulates or knocks out the expression of miR5513 or its precursor in rice susceptible to pests based on target mimicry and CRISPR / Csa9 technology, thereby enhancing the resistance of rice to brown planthopper; compared with traditional chemical control, improving the resistance of crops through genetic engineering is a more environmentally friendly and sustainable solution. It reduces the amount and frequency of pesticide use, reduces environmental pollution and damage to the ecosystem, and the research results of the present application have reference significance for the study of gene molecular function and breeding.

The present invention provides a method for producing a nucleic acid molecule that can obtain a nucleic acid molecule that binds to a target and does not inhibit a function of the target. The production method for a nucleic acid molecule of the present invention is a method for producing a nucleic acid molecule that binds to a first biological molecule and does not inhibit a function of the first biological molecule, the method including the steps of:(A) bringing a candidate nucleic acid molecule into contact with the first biological molecule to select a nucleic acid molecule that has bound to the first biological molecule as a first selected nucleic acid molecule; and(B) selecting the first selected nucleic acid molecule as an intended nucleic acid molecule.

The glycosylation profile of a biologic molecule describes important aspects of such biologic molecule. The characterization of the glycosylation profile of a biologic molecule is an important part of the critical quality attributes in the manufacturing and analytical processes for biologic molecules. As such described herein are methods to determine and characterize the glycosylation profile of a biologic molecule. In particular, the method uses one or more indices to describe the glycosylation profile as a finger-print of such biologic molecule.

The invention discloses a primer sequence for amplifying FhSMT1 gene of fritillaria hupehensis and application of the primer sequence, belongs to the technical field of gene engineering, can effectively amplify CDS sequence of FhSMT1 of fritillaria hupehensis, and further discloses a recombinant expression vector primer sequence of FhSMT1, a recombinant expression vector is constructed through the primer, and the FhSMT1 gene of fritillaria hupehensis is obtained. The FhSMT1 can be subjected to subcellular localization after transformation, and the FhSMT1 gene has important significance in amplification of a CDS sequence of the FhSMT1 of the fritillaria hupehensis, analysis of the molecular function of the FhSMT1 and research of the effect of the FhSMT1 in synthesis of isosteroid alkaloids.

The invention belongs to the technical field of circulating tumor cell detection, and provides a bionic circulating tumor cell detection device. The device comprises a detection molecule, a function construction molecule and a bionic blocking molecule, wherein the molecular structure of the bionic blocking molecule is sulfydryl-A-B; the molecular structure of the function construction molecule is sulfydryl-C-D; the molecular structure of the detection molecule is E-F-G; the A group and the C group are hydrophobic chains; the group B is a bionic amphoteric group; the D group and the E group can be subjected to reversible reaction; the F group is a hydrophilic chain; the G group is a capturing group and can capture circulating tumor cells; the functional construction molecules and the bionic blocking molecules are fixedly connected with the surface gold coating through sulfydryl. According to the device provided by the invention, a biological orthogonal reaction is carried out on the surface of the device to accurately and nondestructively capture the MGE treatment labeled CTC, the captured CTC is nondestructively released through a reversible reaction, and accurate and nondestructive detection of the CTC is realized and cyclic regeneration of the detection device is realized through a detection process of a'labeling-capturing / releasing 'device.

The invention discloses a preparation method and application of a specific membrane material based on a molecular imprinting technology, and belongs to the technical field of sewage treatment. The method realizes preparation of the membrane material through five steps: firstly, preparing a polyvinyl alcohol, polyacrylonitrile or polyvinylidene fluoride nanofiber base membrane by adopting an electrostatic spinning method, and carrying out ethanol ultrasonic cleaning and vacuum drying pretreatment; then preparing a molecular imprinting prepolymer solution containing target template molecules, functional monomers, a cross-linking agent and a solvent according to a molar ratio of 1: (3-6): (0.5-2): (20-40), and doping 1-3% of graphene quantum dots / carboxylated carbon nanotubes to enhance the performance, or optimizing the imprinting effect by adopting a two-step self-assembly method; soaking the base membrane in the pre-polymerization liquid, adding a photonic crystal precursor, and carrying out constant-temperature reaction at 30-60 DEG C for 4-8 hours to establish a surface imprinting and ordered face-centered cubic photonic crystal structure; and finally, carrying out oscillation elution and vacuum drying by using an eluent to obtain the specific membrane material. A precise recognition hole is constructed through a molecular imprinting technology, and the problem that a traditional material is poor in selectivity is solved; and the mass transfer distance is shortened by the nanofiber porous structure.

The application belongs to the technical field of plant genetic engineering, and particularly relates to a rice cytokinin response regulator gene OsRR11 and application. Research finds that OsRR11 encodes a conserved protein containing an aspartic acid signal receiving domain, and is a specific response regulator of cZ in rice; through research, it is found that OsRR11 has a positive regulation function in the resistance of rice to brown planthopper. Through agrobacterium-mediated genetic transformation, OsRR11 is overexpressed in japonica OsRR11 and is knocked out in Bph6-NIL, the resistance of the overexpression plant to brown planthopper is significantly enhanced, and the resistance of the knockout mutant plant to brown planthopper is significantly weakened. The gene of the application provides a good theoretical basis for studying how cytokinin participates in the insect resistance of rice through a response regulator, has a reference significance for studying the molecular function of a gene, and provides a gene resource for molecular design breeding of rice insect resistance.

The invention relates to an RNA (Ribonucleic Acid) co-immunoprecipitation-based RNA binding protein aggregate molecular function detection method, which comprises the following steps: S1) RNA co-immunoprecipitation: firstly, splitting tissues or cells, then cleaning by using magnetic beads to remove a splitting solution, and then preparing a detection sample; s2) protein and RNA detection: firstly carrying out semi-denaturation gel electrophoresis on the sample, confirming the IP efficiency of the sample, and then recovering RNA on the membrane; s3) releasing RNA from the membrane: incubating by adopting a prepared PK buffer solution to perform protein digestion so as to extract RNA, measuring the concentration of RNA, and performing reverse transcription to obtain RNA formed by combining a DAZL polymer and a DAZL monomer; s4) performing qPCR (quantitative polymerase chain reaction) detection on a target target: performing function detection, and verifying whether RNA (ribonucleic acid) exists in the aggregate formed by combining the DAZL polymer and the monomer. The method is simple, efficient and accurate, and provides a more convenient platform for functional research of the polyamyloid protein.

The present invention relates to a mouse-derived OPN protein neutralizing antibody and its application, belonging to the field of biomedicine technology. The antibody comprises a heavy chain and a light chain, the amino acid sequence of the heavy chain signal peptide being shown as SEQ ID NO.1; the amino acid sequence of the heavy chain variable region being shown as SEQ ID NO.2; the amino acid sequence of the heavy chain constant region being shown as SEQ ID NO.3; the amino acid sequence of the light chain signal peptide being shown as SEQ ID NO.4; the amino acid sequence of the light chain variable region being shown as SEQ ID NO.5; and the amino acid sequence of the light chain constant region being shown as SEQ ID NO.6. The present invention provides an OPN protein neutralizing antibody for use in preparing a diagnostic kit for diseases associated with OPN protein, as well as for use in preparing an antibody drug that blocks the function of an OPN molecule.

The application belongs to the field of plant molecular biology and genetic engineering, and specifically discloses a negative regulation Gossypium hirsutum fruit branch length trait gene Gh_A08G254200, which is located on A08 chromosome 122390509~122395098, the gene is composed of two exons and one intron, there is a SNP site at A08:122392954, which is a non-synonymous mutation, the gene is named as GhCSLD3, the CDS sequence length is 3432 bp, the nucleotide sequence of the gene comprises SEQ ID No.1; the amino acid sequence of the encoded protein comprises SEQ ID No.2. In the extreme Gossypium hirsutum non-zero type cotton variety, the gene is silenced, and it is found that the Gossypium hirsutum fruit branch length is elongated, so the gene has a negative regulation effect on the Gossypium hirsutum fruit branch elongation. The verification of the molecular function of the gene has important significance for improving the breeding of Gossypium hirsutum plant type change.

The application discloses a new application of m6A modified gene GAS6 and its receptor MERTK in rheumatoid arthritis. Multi-omics integrated analysis of synovial tissue and peripheral blood mononuclear cells of RA patients reveals common dysregulation of transcriptomics and epitranscriptomics, highlighting genes with both differential expression and m6A modification, which are enriched in processes such as phagocytosis, Th17 differentiation and cell aging. Among these genes, GAS6 shows the most significant m6A hypermethylation and expression up-regulation, and is verified as a key effector molecule interacting with MERTK / AXL receptor. Functional experiments show that GAS6 and MERTK synergistically promote the malignant phenotype of RA fibroblast-like synoviocytes, enhance their proliferation, migration, inflammatory cytokine secretion and anti-apoptotic ability.

The application discloses a nanopore detection probe connected with a target molecule to be detected and a support molecule, and a preparation method and application thereof. The nanopore detection probe is obtained by coupling the target molecule to be detected and the support molecule through a bifunctional cross-linking reagent; wherein the support molecule is composed of a guest interaction molecule, a functional connecting group and a pulling sequence; the guest interaction molecule provides a specific signal for the nanopore generated in a combined detection system of host interaction molecules in a solution; the functional connecting group provides an action site of the bifunctional cross-linking reagent, so as to be combined with the target molecule to be detected; and the pulling sequence provides power for driving the whole probe molecule to enter a nanopore detection area under the action of an electrophoretic force. The target molecule to be detected is detected by using the nanopore detection probe and a nanopore single-molecule technology, so that characteristic signals are generated to perform qualitative and / or quantitative analysis. Taking an amino acid-support molecule as an example, twenty different amino acids can be distinguished by the method.