Algal toxin molecular imprinting chemoreceptor sensor as well as preparation method and application thereof

A technology of chemical receptors and molecular imprinting, applied in the direction of material electrochemical variables, etc., can solve the problems of poor anti-interference ability, achieve the effect of improving stability, reducing costs, and overcoming interference

Active Publication Date: 2015-07-22
永春县产品质量检验所福建省香产品质量检验中心国家燃香类产品质量监督检验中心福建
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrochemical and electrochemiluminescent detection itself has poor anti-interference ability, and the actual sample must be effectively pretreated before it can be used for quantitative analysis, which brings great inconvenience to research users
[0008] In summary, the existing methods for the analysis and detection of algal toxins in water need to be improved urgently, and there is no report in the prior art on the use of molecularly imprinted chemical receptor sensors for the determination of algal toxins in water

Method used

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  • Algal toxin molecular imprinting chemoreceptor sensor as well as preparation method and application thereof
  • Algal toxin molecular imprinting chemoreceptor sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.1 Preparation of molecularly imprinted chemical receptor sensor:

[0026] First, take microcystin, gonylin, acrylic acid, ethylene glycol dimethacrylate and benzoin ethyl ether and mix them according to the molar ratio of 1:1:50:20:1, and mix them uniformly to form a mixed solution. solution at 4°C overnight to make it fully functional.

[0027] Secondly, the glassy carbon electrode is sequentially made of α-Al with a particle size of 1.0 μm, 0.3 μm and 0.05 μm 2 o 3 The powder was polished to the mirror surface, and after cleaning, the glassy carbon electrode was blown dry with nitrogen gas, and 8 μL of the above mixed liquid was drip-coated on the glassy carbon electrode, and irradiated with a UV lamp at 30 cm for 25 min to initiate polymerization to obtain a molecularly imprinted film.

[0028] Finally, the molecularly imprinted membrane was rinsed with 15 mL of 40% ethanol-water mixed solvent and 15 mL of deionized water at a rate of 1 mL / min to remove template ...

Embodiment 2

[0033] 2.1 Preparation of algal toxin molecularly imprinted chemical receptor sensor:

[0034] First, mix microcystin, gonylin, 4-vinylpyridine, N'N'-methylenebisacrylamide, and benzoin ethyl ether in a molar ratio of 1:1:40:15:1.5, and mix well A mixed solution was formed, and the mixed solution was kept overnight at 4° C. to allow it to fully function.

[0035] Secondly, the glassy carbon electrode is sequentially made of α-Al with a particle size of 1.0 μm, 0.3 μm and 0.05 μm 2 o 3 The powder was polished to the mirror surface, and after cleaning, the glassy carbon electrode was blown dry with nitrogen gas, and 12 μL of the above mixed liquid was drip-coated on the glassy carbon electrode, and irradiated with a UV lamp at 30 cm for 15 min to initiate polymerization to obtain a molecularly imprinted film.

[0036] Finally, the molecularly imprinted membrane was rinsed with 15 mL of 50% ethanol-water mixed solvent and 15 mL of deionized water at a rate of 1 mL / min to remove...

Embodiment 3

[0041] 3.1 Preparation of algal toxin molecularly imprinted chemical receptor sensor:

[0042] First, mix microcystin, gonylin, 4-vinylpyridine, N'N'-methylenebisacrylamide, and benzoin ethyl ether in a molar ratio of 1:1:20:10:0.5, and mix well A mixed solution was formed, and the mixed solution was kept overnight at 4° C. to allow it to fully function.

[0043] Secondly, the glassy carbon electrode is sequentially made of α-Al with a particle size of 1.0 μm, 0.3 μm and 0.05 μm 2 o 3 The powder was polished to the mirror surface, and after cleaning, the glassy carbon electrode was blown dry with nitrogen gas, and 10 μL of the above mixed liquid was drip-coated on the glassy carbon electrode, and irradiated with a UV lamp at 25 cm for 10 min to initiate polymerization to obtain a molecularly imprinted film.

[0044] Finally, the molecularly imprinted membrane was rinsed with 15 mL of 50% ethanol-water mixed solvent and 15 mL of deionized water at a rate of 1 mL / min to remove...

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Abstract

The invention relates to an algal toxin molecular imprinting chemoreceptor sensor. The sensor comprises a three-electrode system, wherein a glassy carbon electrode is a work electrode, a platinum wire electrode is an auxiliary electrode, and Ag/AgCl is a reference electrode; the glassy carbon electrode is modified with an algal toxin molecular imprinting film which comprises algal toxin mixed template modules, function monomers, crosslinking agents and photoinitiators in the mole ratio of 1: (20-50): (10-20): (0.5-1.5). The invention further relates to a preparation method of the sensor and an application of the sensor for analysis and detection of content of algal toxin in water. The sensor is used for analysis and detection of the algal toxin in the water, operation is convenient and fast, the flexibility is high, the operation cost is low, and the sensor is easy to control and automatic, can be used for quick screening of algal toxin in the water and has high use value in fields of environment monitoring and safety control of drinking water.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring and analytical chemical detection, in particular to a algae toxin molecularly imprinted chemical receptor sensor for algae toxin detection in water bodies, a preparation method and application thereof. Background technique [0002] Eutrophication of water often leads to explosive growth of plankton (algae), forming serious water pollution problems such as red tide, blue tide, and water bloom. widespread attention. There are dozens of common algal toxins, plus hundreds of isomers, such as microcysin (MC), nodularin (nodularin), and cylindromycin ( cylindrospermopsin), anatoxin-a(an-atoxin-a), anatoxin-a(s)[anatoxin-a(s)], saxitoxin, neolithin Saxitoxin (neosaxitoxin), gonyautoxin (gonyautoxin), etc. After drinking water containing algal toxins, symptoms such as diarrhea, fatigue, anorexia, vomiting, lethargy, increased mouth and eye secretions, and even death may occur. Long-ter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 吴文文王邃
Owner 永春县产品质量检验所福建省香产品质量检验中心国家燃香类产品质量监督检验中心福建
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