Sunset yellow molecular imprinting detection electrochemical sensor preparation method

A technology of molecular imprinting and sunset yellow, which is applied in the field of electrochemistry and analytical chemistry, can solve problems such as the influence of experimental results, high detection limit, and complicated processing process, and achieve enhanced conductivity and sensitivity, excellent conductivity, and good conductivity. and the effect of electrocatalytic performance

Inactive Publication Date: 2016-02-24
UNIV OF JINAN
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Problems solved by technology

At present, there are high-performance liquid chromatography, thin-layer chromatography, polarography, and spectrophotometry for the detection of sunset yellow, but these methods are cumbersome to operate, complicated in the pretreatment process, and high in analysis costs, and cannot achieve rapid and real-time detection
The patent application number is CN201310478241, which discloses a method for detecting sunset yellow by ultraviolet-visible spectrophotometry. This method extracts the sample and measures the absorbance of the organic phase. have a certain influence

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  • Sunset yellow molecular imprinting detection electrochemical sensor preparation method

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Effect test

Embodiment 1

[0022] Preparation of sunset yellow molecularly imprinted polymer: 50mg graphene oxide, 50mg anhydrous ferric chloride, 35mg ferrous chloride, N 2 Protect, add dropwise ammonia to pH 9.0, then add 20.0μL of hydrazine hydrate, shake for a few minutes, and heat at 60°C for 3.5h to separate and obtain magnetic graphene, 10mg magnetic graphene, 10mg 1-butyl-3-methylimidazole hydrogen Bromate, 15mL of nano-gold sol, sonicated in ethanol solution for 30min, and dried at 30°C to obtain the product MG-IL-AuNPs. 0.2mmol sunset yellow, 0.1g MG-IL-AuNPs, 0.8mmol methacrylic acid, methanol-water (volume ratio 4:1) solution pre-polymerized for 2h, add 4mmol ethylene glycol dimethacrylate, 50mL acetonitrile and 20mg coupling Nitrogen diisobutyronitrile, deoxidize with nitrogen, shake at 60°C for 24h. Then wash with methanol-acetic acid (volume ratio 9:1) solution until colorless. The product was washed with ethanol to neutrality, and dried under vacuum at 50°C to obtain sunset yellow molecu...

Embodiment 2

[0025] Preparation of sunset yellow molecularly imprinted polymer: 60mg graphene oxide, 55mg anhydrous ferric chloride, 40mg ferrous chloride, N 2 Protect, add dropwise ammonia to pH 9.0, then add 25.0μL of hydrazine hydrate, shake for a few minutes, heat at 60°C for 4h, and separate and obtain magnetic graphene, 15mg magnetic graphene, 20mg 1-butyl-3-methylimidazole hydrobromide MG-IL-AuNPs were obtained by sonicating for 30 min in ethanol solution, 20 mL of nano gold sol, and drying at 30 °C. 0.3mmol sunset yellow, 0.2g MG-IL-AuNPs, 1mmol methacrylic acid, methanol-water (volume ratio 4:1) solution pre-polymerized for 2.5h, add 5mmol ethylene glycol dimethacrylate, 50mL acetonitrile and 20mg coupling Nitrogen diisobutyronitrile, deoxidize with nitrogen, shake at 60°C for 24h. Then wash with methanol-acetic acid (volume ratio 9:1) solution until colorless. Wash the product with ethanol to neutrality and dry it at 50°C to obtain sunset yellow molecularly imprinted polymer;

[0...

Embodiment 3

[0028] Detection of sunset yellow in Mininda beverage samples by molecularly imprinted electrochemical sensor:

[0029] The modified electrode MIPs / GCE is used as the working electrode, the Ag / AgCl electrode is the reference electrode, and the platinum electrode is the counter electrode. Different concentrations of sunset yellow standard solution samples are subjected to differential pulse scanning in the potential range of 0.2~1.0V, according to the peak current The relationship between the value and the concentration of sunset yellow;

[0030] The linear range of sunset yellow is 1.0×10 -9 ~2.0×10 -6 molL -1 , The linear equation is Δ I p =0.09205+0.17957 c , c Is the concentration, the unit is μmolL -1 , Peak current I p The unit is μA, the detection limit is 3×10 -10 molL -1 ;

[0031] Detection of sunset yellow in Mirinda beverages: The modified electrode MIPs / GCE is used as the working electrode, the Ag / AgCl electrode is the reference electrode, and the platinum electrode is th...

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Abstract

The present invention discloses a sunset yellow molecular imprinting detection electrochemical sensor preparation method. In the method, magnetic graphene is used as a carrier, the surface is modified with 1-butyl-3-methyl imidazole hydrobromide ionic liquid and nano Au, and a sunset yellow molecular imprinting polymer is prepared by addition of a sunset yellow template molecule, a functional monomer and a crosslinking agent on the basis of the polymer. The glassy carbon electrode surface is modified with the sunset yellow molecular imprinting polymer for construction of a sunset yellow molecular imprinting detection electrochemical sensor. The electrochemical sensor constructed on the basis of the imprinted material has high sunset yellow detection selectivity and sensitivity, and can achieve rapid and sensitive detection of a real sample.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted electrochemical sensor for detecting sunset yellow, belonging to the technical fields of electrochemistry and analytical chemistry. Background technique [0002] Sunset yellow is a food additive approved for use in China. It is mainly used for coloring food and medicine. Vegetable protein beverages and lactic acid bacteria beverages, pickled side dishes, puffed foods and other foods. If a person consumes a large amount of food with excessive sunset yellow pigment content for a long time or at one time, symptoms such as allergies and diarrhea will occur. When the intake is too large and exceeds the liver load, it will accumulate in the body and cause certain damage to the kidneys and liver. The Food Additives Joint Expert Committee of the World Food and Agriculture Organization and the World Health Organization, an authoritative organization for food additive safety evaluation, ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
Inventor 李剑波罗川南王晓娇段辉敏王延辉孙元玲丁超凡孙敏冯娟娟步亚南
Owner UNIV OF JINAN
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