Application of mycotoxin biodegradation agent to promotion of healthy pig production
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A technology of biodegradation agent and mycotoxins, applied in the biological field, can solve the problems of unbalanced flora and damage to animals caused by mycotoxins, and achieve the effect of increasing daily weight gain
Inactive Publication Date: 2020-01-14
HENAN ZILONG BIOTECH +2
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[0004] For this reason, the embodiment of the present invention provides a mycotoxin biodegradation agent, a preparation method and its application, so as to solve the problems in the prior art that the flora is unbalanced and mycotoxins cause damage to animals
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Embodiment 1
[0045] 1. Test materials and methods
[0046]1.1 Experimental animal grouping and feeding management: The experimental location is a farm in Xinxiang, Henan. According to the principles of the same breed, similar parity, and similar body weight, 150 weaned “Du × Chang × Da” three-way hybrid piglets with an average weight of 11.03 kg were selected. They were randomly divided into 5 groups, with 3 repetitions in each group, 10 heads / replication, half male and half female, and each repetition was reared in a single pen. The feeding experiment was carried out for 60 days.
[0047] 1.2 Preparation of mycotoxin biodegradants
[0048] In the examples of the present invention, Lactobacillus (CGMCC1.2884), Bacillus subtilis (CGMCC1.0504) and Candida utilis (CGMCC2.0615) Aspergillus oryzae strains (preservation number CGMCC3.4437) were all purchased from China Microorganisms Culture Collection Management Committee General Microbiology Center.
[0049] Preparation of compound probiot...
Embodiment 2
[0081] 1 Test materials and methods
[0082] 1.1 Test material: The test sample comes from the liver tissue of piglets fed for 60 days, three repetitions in the three groups of A group (control group), D (AFB1+ZEA group) and E (AFB1+ZEA+mycotoxin biodegradant group) In each repeated slaughtering of a piglet, the liver was taken from the same position, and the blood on the tissue was rinsed with pre-cooled PBS, then quickly stored in liquid nitrogen, and then transferred to -80°C for independent data collection (Data-independent Acquisition, DIA) proteomic analysis.
[0083] 1.2 Protein extraction: all 9 sample proteins were extracted by cold acetone method, the samples were ground into powder in liquid nitrogen, and then dissolved in 2 mL lysate (7M urea, 2% SDS, 0.1% PMSF (Roche Ltd.Basel, Switzerland) , 65mMDTT), followed by ultrasonic homogenization on ice, 3min / time, a total of 3 times. Centrifuge at 15,000 rpm for 15 min at 4°C, and transfer the supernatant to a new EP ...
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Abstract
The embodiment of the invention relates to application of a mycotoxin biodegradation agent to promotion of healthy pig production, in particular to application of the mycotoxin biodegradation agent tothe preparation of feed for positively regulating a microbial flora in contents of pig jejunum, improving the detoxification ability of pigs and promoting healthy growth. The mycotoxin biodegradationagent can up-regulate the expression of 153 proteins in the liver of piglets and down-regulate the expression of 55 proteins. The differential proteins are mainly involved in the composition of cellcomponents, molecular functions and biological processes. Analysis of KEGG pathways of the differential proteins shows that the mycotoxin biodegradation agent can up-regulate the expression of 13 proteins without down-regulated proteins, the KEGG pathways in which the up-regulated proteins mainly participate include endoplasmic reticulum protein processing, glycolysis/gluconeogenesis, tryptophan metabolism, valine, leucine and isoleucine metabolism, microbial metabolism in different environments, starch and sucrose metabolism and the like.
Description
technical field [0001] The embodiment of the present invention relates to the field of biotechnology, in particular to the application of a mycotoxin biodegradation agent in promoting the healthy growth of pigs. Background technique [0002] At present, about 100 kinds of fungi produce about 300 kinds of toxic metabolites - mycotoxins. Crop products, feed ingredients and animal feed are easily contaminated with mycotoxins, causing great harm to the food industry, feed industry and animal husbandry production. [0003] Although people have taken various measures to inhibit bacteria and remove mycotoxins, they have had little effect, and the situation of feed being contaminated by mycotoxins is still quite common. In addition to endangering animals, these mycotoxins can also enter the food chain through contaminated grains and residues in animal foods (meat, eggs, milk, etc.), thereby endangering human health and life. Mycotoxins have serious problems such as large quantity,...
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