El-b3a gene in soybean growth period and coding protein thereof

A technology of e1-b3a and encoded protein, applied in the field of soybean growth period e1-b3a gene and its encoded protein, can solve the problems affecting soybean growth period and so on

Inactive Publication Date: 2012-09-26
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In soybean cultivars, how the mutation of the E1 gene affects the soybean growth period (flowering period and ma...

Method used

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  • El-b3a gene in soybean growth period and coding protein thereof
  • El-b3a gene in soybean growth period and coding protein thereof
  • El-b3a gene in soybean growth period and coding protein thereof

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Experimental program
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Effect test

specific Embodiment approach 1

[0013] Embodiment 1: The mutant gene sequence of the soybean growth stage e1-b3a gene of this embodiment is shown in the sequence table Seq ID No: 1.

[0014] In this embodiment, on the basis of cloning the soybean growth stage E1 gene (invention patent application number: 201210112662.9), further in-depth and systematic research on the E1 gene sequence is carried out. Because there is a typical B3 gene domain gene in soybean, this gene represents a new type of gene family. We have confirmed that the E1 gene can represent the function of the E1 site, that is, it has the function of inhibiting soybean flowering. In this embodiment, the E1 gene sequences in different varieties and near-isogenic lines are studied, and the e1-b3a genes are determined, which can promote soybean flowering.

[0015] The soybean growth stage E1 gene has a region similar to the B3 domain in the region of amino acid sequence 55-174. The nucleotide sequence of the soybean growth stage e1-b3a gene has thr...

specific Embodiment approach 2

[0016] Embodiment 2: The amino acid sequence of the encoded protein of the soybean growth stage e1-b3a gene in this embodiment is shown in Seq ID No: 2 in the sequence table.

[0017] In this embodiment, on the basis of cloning the soybean growth stage E1 gene (invention patent application number: 201210112662.9), further in-depth and systematic research on the E1 gene sequence is carried out. Because there is a typical B3 gene domain gene in soybean, this gene represents a new type of gene family. We have confirmed that the E1 gene can represent the function of the E1 site, that is, it has the function of inhibiting soybean flowering. In this embodiment, the E1 gene sequences in different varieties and near-isogenic lines are studied, and the e1-b3a genes are determined, which can promote soybean flowering.

[0018] The soybean growth stage E1 gene has a region similar to the B3 domain in the region of amino acid sequence 55-174. The nucleotide sequence of the soybean growth ...

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Abstract

The invention relates to an el-b3a gene in a soybean growth period and a coding protein thereof. The gene sequence of the el-b3a gene in the soybean growth period is shown as a sequence list Seq ID No:1, and the amino acid sequence of the coding protein of the el-b3a gene in the soybean growth period is shown as a sequence list Seq ID No:2. By means of relevant heredity and molecular functional verification of varieties, the el-b3a gene has a flowering promoting function as compared with El genes.

Description

technical field [0001] The invention relates to soybean growth stage e1-b3a gene and its encoded protein. Background technique [0002] The E1 gene is located near the centromere, and no functional gene has been cloned since Bernard (1971) reported it. Since the gene is located near the centromere (pericentromeric region), the genetic recombination rate is low, which is not conducive to the use of map-based cloning to clone the gene. The name of the invention patent: a soybean growth stage E1 gene and its encoded protein, patent application No.: 201210112662.9, after publishing the E1 gene sequence and its corresponding protein sequence, it is proved that the soybean growth stage E1 gene has a strong physiological function of inhibiting soybean flowering. Although its protein sequence cannot identify the B3 domain, it has a distant relationship with the B3 domain gene. The B3 domain gene is a relatively conserved plant-specific transcription factor, generally only 100-120 ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 夏正俊吕世翔翟红吴红艳刘宝辉邱丽娟
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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