Application of taking oligopeptide and derivatives thereof as template molecule, and imprinting technology based preparation method of angiotensin synthetic receptor
An angiotensin and template molecule technology, applied in the field of biomedicine, can solve the problems of complex thermodynamic factors in the structure of biological macromolecules, limited successful examples of peptides and proteins, and difficulty in obtaining molecularly imprinted polymers, etc., to achieve high imprinting effect, affinity and The effect of high selectivity and simple process
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Embodiment 1
[0032] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules....
Embodiment 2
[0037] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules...
Embodiment 3
[0044] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules...
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