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Application of taking oligopeptide and derivatives thereof as template molecule, and imprinting technology based preparation method of angiotensin synthetic receptor

An angiotensin and template molecule technology, applied in the field of biomedicine, can solve the problems of complex thermodynamic factors in the structure of biological macromolecules, limited successful examples of peptides and proteins, and difficulty in obtaining molecularly imprinted polymers, etc., to achieve high imprinting effect, affinity and The effect of high selectivity and simple process

Inactive Publication Date: 2013-11-27
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most successful application of molecular imprinting technology is the identification of small organic molecules, and the successful examples of identifying and capturing peptides and proteins are very limited. The reasons are due to the complex structure of biological macromolecules and thermodynamic factors; another main reason is that peptides and proteins are expensive. , it is often difficult to obtain sufficient quantities for the preparation of molecularly imprinted polymers
[0007]Currently, there have been reports on the synthesis of molecularly imprinted nanogels with a size similar to that of natural enzymes or antibodies (<100nm), but using angiotensin as a ligand The imprinted nanogel receptors are still unreported

Method used

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  • Application of taking oligopeptide and derivatives thereof as template molecule, and imprinting technology based preparation method of angiotensin synthetic receptor
  • Application of taking oligopeptide and derivatives thereof as template molecule, and imprinting technology based preparation method of angiotensin synthetic receptor
  • Application of taking oligopeptide and derivatives thereof as template molecule, and imprinting technology based preparation method of angiotensin synthetic receptor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules....

Embodiment 2

[0037] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules...

Embodiment 3

[0044] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol acrylic acid, 0.156 mmol N-isopropylacrylamide were dissolved in 50 mL distilled water to obtain solution 1. 0.065 mmol N-tert-butylacrylamide was dissolved in 1 mL absolute ethanol to obtain solution 2, and solution 2 was mixed Into the above solution 1, after shaking for 4 hours, the solution 3 was obtained. Add 1 mg N, N-methylenebisacrylamide and 10 mg sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add initiator ammonium peroxosulfate 30 mg, 15 μL of N, N, N, N -Tetramethyldiethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO 10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted monomers and template molecules...

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Abstract

The invention discloses an application of taking oligopeptide and derivatives thereof as a template molecule, and an imprinting technology based preparation method of an angiotensin synthetic receptor. The template molecule comprises any section of a peptide bond at a nitrogen end or a carbon end of an angiotensin II, and derivatives thereof, or any section of the peptide bond at the nitrogen end or the carbon end of an angiotensin I and the derivatives thereof. The application takes the template molecule as an imprint to prepare the angiotensin synthetic receptor; the angiotensin acceptor is prepared in a way that the template molecule, a function monomer, a cross-linking agent, a surface-active agent and the like are mixed according to a certain rate, and then an initiator is added. The acceptor is good in biocompatibility, can high selectively capture and neutralize the angiotensin II and the angiotensin I, and cuts off functions of the angiotensin II and the angiotensin acceptor (AT1) as well as functions of the angiotensin I and angiotensin converzyme (ACE), so as to control the blood pressure. The acceptor can be applied to separation, concentration and measuring of the angiotensin I or II.

Description

[0001] technical field [0002] The invention relates to the technical field of biomedicine and the technical field of separation, enrichment, analysis and detection, in particular to the application of a short peptide and its derivatives as template molecules and a method for preparing an angiotensin synthesis receptor based on imprinting technology. Background technique [0003] As the main bioactive peptide of the renin-angiotensin-aldosterone system (RAS), overexpression of angiotensin II in vivo can cause serious physical diseases. Angiotensin Ⅱ can stimulate the adrenal cortical zona cells to synthesize and release aldosterone. Aldosterone acts on the renal tubules to retain sodium, water, and excrete potassium, causing increased blood volume, constricting blood vessels, and raising blood pressure; it can also act on vascular tension. Angiotensin II can also increase the concentration of Ca ions by acting on the Na-Ca channel of the cell, causing vasoconstriction, ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/54C08F220/06C08J9/26B01J20/26B01J20/30
Inventor 汤又文余泽荣徐志广
Owner SOUTH CHINA NORMAL UNIVERSITY
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