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Method for preparing angiotensin synthesis receptor based on blotting technology and prepared angiotensin synthesis receptor

An angiotensin and technology technology, which is applied in the field of preparing angiotensin synthesis receptors based on imprinting technology, can solve the problems of complex thermodynamic factors of biological macromolecular structures, limited successful examples of polypeptides and proteins, and difficulty in obtaining molecularly imprinted polymers, etc. Achieve high blotting effect, high affinity and selectivity, and simple process

Inactive Publication Date: 2016-01-20
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most successful application of molecular imprinting technology is the identification of small organic molecules, and the successful examples of identifying and capturing peptides and proteins are very limited. The reasons are due to the complex structure of biological macromolecules and thermodynamic factors; another main reason is that peptides and proteins are expensive. , it is often difficult to obtain sufficient quantities for the preparation of molecularly imprinted polymers
[0006]Currently, there have been reports on the synthesis of molecularly imprinted nanogels with a size similar to that of natural enzymes or antibodies (<100nm), but using angiotensin as a ligand The imprinted nanogel receptors are still unreported

Method used

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  • Method for preparing angiotensin synthesis receptor based on blotting technology and prepared angiotensin synthesis receptor
  • Method for preparing angiotensin synthesis receptor based on blotting technology and prepared angiotensin synthesis receptor
  • Method for preparing angiotensin synthesis receptor based on blotting technology and prepared angiotensin synthesis receptor

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Experimental program
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Effect test

Embodiment 1

[0030] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol of acrylic acid, 0.156 mmol of N-isopropylacrylamide were dissolved in 50 mL of distilled water to obtain solution 1. 0.065 mmol of N-tert-butylacrylamide was dissolved in 1 mL of absolute ethanol to obtain solution 2, and solution 2 was mixed into the above solution In 1, solution 3 was obtained after shaking for 4 hours. Add 1 mg of N, N-methylene bisacrylamide and 10 mg of sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add 30 mg of initiator ammonium peroxosulfate, 15 μL of N, N, N, N-tetramethyl base diethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted mono...

Embodiment 2

[0034] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol of acrylic acid, 0.156 mmol of N-isopropylacrylamide were dissolved in 50 mL of distilled water to obtain solution 1. 0.065 mmol of N-tert-butylacrylamide was dissolved in 1 mL of absolute ethanol to obtain solution 2, and solution 2 was mixed into the above solution In 1, solution 3 was obtained after shaking for 4 hours. Add 1 mg of N, N-methylene bisacrylamide and 10 mg of sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add 30 mg of initiator ammonium peroxosulfate, 15 μL of N, N, N, N-tetramethyl base diethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted mono...

Embodiment 3

[0040] 0.0045g of the template molecule, namely the N-terminal tripeptide derivative template of angiotensin II (Asp-Arg-Val-NHC 2 h 5 ), 0.098 mmol of acrylic acid, 0.156 mmol of N-isopropylacrylamide were dissolved in 50 mL of distilled water to obtain solution 1. 0.065 mmol of N-tert-butylacrylamide was dissolved in 1 mL of absolute ethanol to obtain solution 2, and solution 2 was mixed into the above solution In 1, solution 3 was obtained after shaking for 4 hours. Add 1 mg of N, N-methylene bisacrylamide and 10 mg of sodium dodecyl sulfate to the above solution 3, pass nitrogen gas for 30 minutes, add 30 mg of initiator ammonium peroxosulfate, 15 μL of N, N, N, N-tetramethyl base diethylamine, sealed, and reacted under nitrogen atmosphere at room temperature for 24 hours. Transfer the reaction solution into a dialysis bag (MWCO10000), and perform dialysis in a large amount of distilled water for four days, changing the water at least twice a day to remove unreacted mono...

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Abstract

The invention discloses the application of a short peptide and its derivatives as a template molecule and a method for preparing an angiotensin synthesis receptor based on imprinting technology. The template molecule includes any peptide bond at the nitrogen end or carbon end of angiotensin II and its derivatives, or any peptide bond at the nitrogen end or carbon end of angiotensin I and its derivatives. Said application is to prepare angiotensin synthesis receptor by molecular imprinting of the above template. The angiotensin receptor is prepared by mixing template molecules, functional monomers, cross-linking agents and surfactants in a certain proportion, adding an initiator and then polymerizing. The receptor has good biocompatibility, can capture and neutralize angiotensin Ⅱ and angiotensin Ⅰ with high selectivity, block angiotensin Ⅱ and angiotensin receptor (AT1) and angiotensin Ⅰ and angiotensin The role of acetaminophen converting enzyme (ACE), so as to achieve the purpose of controlling blood pressure. The receptor can also be used for separation, enrichment and determination of angiotensin I or II.

Description

technical field [0001] The invention relates to the technical field of biomedicine and the technical field of separation, enrichment, analysis and detection, in particular to the application of a short peptide and its derivatives as template molecules and a method for preparing an angiotensin synthesis receptor based on imprinting technology. Background technique [0002] As the main bioactive peptide of the renin-angiotensin-aldosterone system (RAS), overexpression of angiotensin II in vivo can cause serious physical diseases. Angiotensin Ⅱ can stimulate the adrenal cortical zona cells to synthesize and release aldosterone. Aldosterone acts on the renal tubules to retain sodium, water, and excrete potassium, causing increased blood volume, constricting blood vessels, and raising blood pressure; it can also act on vascular tension. Angiotensin II can also increase the concentration of Ca ions by acting on the Na-Ca channel of the cell, causing vasoconstriction, thereby incre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/54C08F220/06C08J9/26B01J20/26B01J20/30
Inventor 汤又文余泽荣徐志广
Owner SOUTH CHINA NORMAL UNIVERSITY
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