Hollow porous medium for separating and enriching taxanes as well as preparation and application of hollow porous medium

A porous medium, separation and enrichment technology, applied in other chemical processes, chemical instruments and methods, etc., can solve the problems of unavailability of binding sites, serious entrapment, and low efficiency of taxane separation and enrichment. Imprinting and adsorption performance, improved utilization, easy-to-implement effects

Active Publication Date: 2022-01-14
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the defects of the prior art, the present invention provides a hollow porous medium for efficient and specific separation and enrichment of taxanes, its preparation method and application. The hollow porous medium for efficient and specific separation of taxanes has the following characteristics: A surface molecularly imprinted polymer layer with a certain thickness, and a large number of binding sites that are partially or completely identical to the spatial structure of paclitaxel, thereby solving the problem of the prior art molecularly imprinted polymer material binding site embedding for separation and enrichment of taxanes Seriously, a large number of binding sites cannot be used, and the technical problems of taxane separation and enrichment efficiency are low

Method used

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  • Hollow porous medium for separating and enriching taxanes as well as preparation and application of hollow porous medium
  • Hollow porous medium for separating and enriching taxanes as well as preparation and application of hollow porous medium
  • Hollow porous medium for separating and enriching taxanes as well as preparation and application of hollow porous medium

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preparation example Construction

[0033] A method for preparing a hollow porous medium for separating taxanes provided by the invention, such as figure 1 shown, including the following steps:

[0034] (1) Mixing the silica gel particles activated by the acid solution with a silylating agent, modifying the silica gel particles, washing and drying to obtain the modified silica gel particles;

[0035] (2) Mix the modified silica gel particles with the porogen, and add template molecules, functional monomers, crosslinking agents, cage silsesquioxanes and initiators, mix and degas, and heat and stir The polymerization reaction is carried out under the following conditions, and after the reaction is completed, the surface molecularly imprinted polymer material is obtained by filtering, washing and drying;

[0036](3) Add hydrofluoric acid solution to the surface molecularly imprinted polymer material obtained in step (2), filter after stirring, rinse with pure water until neutral and then dry to obtain a hollow por...

Embodiment 1

[0057] A method for preparing a porous medium for efficiently and specifically separating taxanes, comprising the steps of:

[0058] (1) Silica gel activation and modification treatment

[0059] Weigh 10g of amorphous silica gel, add 200mL of 20wt% dilute nitric acid solution to stir, filter, rinse with pure water until neutral, and dry to obtain activated amorphous silica gel particles P1A; add 10g of P1A to 200mL of toluene solution, add 0.33 mL of vinyltrimethoxysilane was reacted for 24 hours to obtain modified silica gel P1B.

[0060] (2) Surface molecular imprinting technology treatment

[0061] Weigh 0.05mmol of paclitaxel and 0.5mmol of functional monomer methacrylic acid, fully dissolve in 20mL of dichloromethane, and ultrasonically mix for 10min. Add 1g of the above-mentioned P1B, 1mmol of crosslinking agent ethylene glycol dimethacrylate, 0.05mmol of octaphenyl-POSS, and 20mg of initiator AIBN, mix well, ultrasonication for 10min, and nitrogen degassing for 30min....

Embodiment 2

[0070] A method for preparing a porous medium for efficiently and specifically separating taxanes, comprising the steps of:

[0071] (1) Silica gel activation and modification treatment

[0072] Weigh 10g of amorphous silica gel, add 200mL of 35wt% dilute nitric acid solution to stir, filter, rinse with pure water until neutral, and dry to obtain activated amorphous silica gel particles P2A; take 10g of 2A and add it to 200mL of toluene solution, add 0.67 mL methacryloyloxypropyltrimethoxysilane, reacted for 24h to obtain modified silica gel P2B.

[0073] (2) Surface molecular imprinting technology treatment

[0074]Weigh 1g of P2B, add 0.05mmol paclitaxel and 0.4mmol functional monomer 2-vinylpyridine, fully dissolve in 20mL toluene, and mix by ultrasonic for 10min. Add 2mmol cross-linking agent maleic rosin acrylate, 0.2mmol octavinyl-POSS, and add 40mg initiator AIBN, mix thoroughly, ultrasonic 10min, nitrogen degassing 30min. Put the round-bottomed flask into a magnetic...

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Abstract

The invention belongs to the technical field of molecularly imprinted polymers, and particularly relates to a hollow porous medium for separating and enriching taxanes as well as preparation and application of the hollow porous medium. The preparation comprises the steps: firstly, grafting a silanization reagent with double bonds on the surfaces of silica gel particles, then forming a molecularly imprinted polymer layer with a certain thickness on the surfaces of the silica gel particles by utilizing a surface molecular imprinting technology, and finally, amorphous silica gel and a cage-like silsesquioxane reagent in the molecularly imprinted polymer layer are corroded by utilizing hydrofluoric acid; therefore, the hollow porous medium which is high in specific surface area and porosity and has high selective adsorption performance on taxane structures is obtained. The hollow porous medium obtained by the process has high adsorptive selectivity on various taxane substances at the same time, can realize efficient and synchronous separation and purification to obtain various taxanes, and has process application value.

Description

technical field [0001] The invention belongs to the technical field of molecularly imprinted polymers, and more specifically relates to a hollow porous medium for separating and enriching taxanes, its preparation and application. Background technique [0002] Paclitaxel is a diterpenoid compound, as a natural anticancer drug with high efficiency, broad spectrum, strong activity and unique mechanism of action, especially for ovarian cancer, breast cancer, non-small cell lung cancer, etc., and is gradually promoted into more cancer treatments. However, because the content of paclitaxel in the branches and leaves of Taxus chinensis is very low, accounting for about 0.005-0.06% of the dry weight of the plant, and there are also a large number of taxane compounds similar in structure to paclitaxel. High-purity paclitaxel can only be obtained by combining silica gel column chromatography with reverse preparative chromatography system, but it is impossible to simultaneously obtain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F283/12C08F220/06C08F222/14C08F289/00C08F226/06C08J9/26B01J20/26B01J20/30
CPCC08F292/00C08F283/12C08F283/124C08F289/00C08J9/26B01J20/286C08F220/06C08F222/102C08F226/06C08F222/103
Inventor 张华余龙江付春华赵春芳徐航
Owner HUAZHONG UNIV OF SCI & TECH
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