Molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residue and application thereof

A technology of molecular imprinting and pesticide residues, applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of high technical cost, inability to detect quickly, and not suitable for rapid detection, and achieve high sensitivity and Detection range, avoiding the influence of subjective factors, and realizing the effect of high-throughput screening

Inactive Publication Date: 2010-02-10
UNIV OF JINAN
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Problems solved by technology

[0006] 1. Uterine weight gain test and uterine calcium-binding protein CaBP-9k mRNA expression detection and protein analysis are often used in in vivo tests. This method is sensitive and specific, but its cost is high and its sensitivity is low, so it is not suitable for large-scale rapid screening ;
[0007] 2. In vitro tests mainly include E-Screen method, receptor competition test, etc. The specificity of the test itself is poor, and false positive results are prone to occur;
[0008] 3. The instrumental detection method for pesticide residues established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the det...

Method used

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  • Molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residue and application thereof
  • Molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residue and application thereof
  • Molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residue and application thereof

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Embodiment 1

[0038] Embodiment 1 (organophosphorus, such as trichlorfon)

[0039] A method for preparing a molecularly imprinted membrane chemiluminescent sensor for detecting trichlorfon, comprising the following steps:

[0040] (1) Select functional monomers phenyltrimethoxysilane (PTMOS) and methyltrimethoxysilane (MTMOS) that can synthesize MIPs with trichlorfon;

[0041] (2) Preparation of CdTe quantum dot solution: in N 2 Under protection, the existing NaHTe is used as Te precursor, and CdCl 2 Reaction, under the condition of thioglycolic acid as stabilizer, the water-soluble CdTe quantum dot solution of making;

[0042] (3) Take 20 μl of the prepared CdTe solution and sonicate for 20 minutes to obtain a uniformly dispersed CdTe quantum dot solution;

[0043] (4) Template molecule trichlorfon, functional monomers phenyltrimethoxysilane (PTMOS) and methyltrimethoxysilane (MTMOS), cross-linking agent ethylene glycol dimethacrylate (EGDMA), porogen Chloroform, initiator azobisisobut...

Embodiment 2

[0051] Embodiment 2 (carbamate, as carbofuran)

[0052] A method for preparing a molecularly imprinted membrane chemiluminescent sensor for detecting carbofuran, comprising the following steps:

[0053] (1) select the functional monomer acrylamide (MA) that can synthesize MIPs with carbofuran;

[0054] (2) Preparation of CdSe quantum dot solution: in N 2 Under protection, NaHSe is the precursor of Se, and CdCl 2 Reaction, under the condition of thioglycolic acid as stabilizer, the water-soluble CdSe quantum dot solution of making;

[0055] (3) Take 100 μL of the prepared CdSe solution and sonicate for 30 minutes to obtain a uniformly dispersed CdSe quantum dot solution;

[0056] (4) Template molecule carbofuran, functional monomer acrylamide (MA), crosslinking agent ethylene glycol dimethacrylate (EGDMA), porogen carbon tetrachloride, initiator azobisisobutyronitrile, silicon The source tetraethyl orthosilicate is mixed uniformly in a molar ratio of 0.1:1:20:30:0.05:20 to ...

Embodiment 3

[0064] Embodiment 3 (organic nitrogen, such as dimeform)

[0065] A method for preparing a molecularly imprinted membrane chemiluminescent sensor for detecting dimeform, comprising the following steps:

[0066] (1) select the functional monomer methacrylic acid (MAA) that can synthesize MIPs with dimeform;

[0067] (2) Preparation of CdSe quantum dot solution: in N 2 Under protection, NaHSe is the precursor of Se, and CdCl 2 Reaction, under the condition of mercaptopropionic acid as stabilizer, the water-soluble CdSe quantum dot solution of making;

[0068] (3) Take 100 μL of the prepared CdSe solution and sonicate for 30 minutes to obtain a uniformly dispersed CdSe quantum dot solution;

[0069] (4) template molecule dimeform, functional monomer methacrylic acid (MAA), crosslinking agent N, N-methylenebisacrylamide, porogen methanol, initiator azobisisobutyronitrile, silicon source positive Ethyl silicate is mixed uniformly at a molar ratio of 0.5:1:10:40:0.1:25 to obtain...

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Abstract

The invention relates to a molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residues, belonging to the technical field of the detection of the pesticide residues. Theinvention also relates to a method for detecting the trace amount pesticide residues contained in agricultural product samples by using the molecular imprinting chemiluminescence sensor, which comprises the following steps: (1) selecting a functional monomer; (2) uniformly mixing a template molecule, the functional monomer, a cross linker, a pore-forming agent, an initiating agent and a silicon source to prepare an MIPs collosol according to a mole ratio of 0.1-1 to 1-10 to 5-25 to 20-60 to 0.05-0.15 to 20-50; (3) preparing a quantum dot material solution; and (4) modifying the MIPs collosoland quantum dot materials to the surface of a millipore plate to manufacture the chemiluminescence sensor by a layer-layer self-assembly surface modification technique. The invention has the advantages of high specificity and sensitivity, fast detection, low cost, good repeatability and convenient field detection on the detection of the pesticide residues.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue detection, more specifically, a molecularly imprinted chemiluminescent sensor for detecting trace amounts of pesticide residues, and the present invention also relates to the use of the molecularly imprinted chemiluminescent sensor to detect trace amounts in agricultural product samples methods of pesticide residues. Background technique [0002] Pesticide residues mainly include insecticides, fungicides, herbicides, etc. according to different uses of pesticides. Pesticide residues interfere with the normal behavior of organisms and the synthesis, storage, secretion, transport, combination, and clearance of normal hormones related to reproduction and development. [0003] At present, pesticide residues have become a new generation of environmental pollutants and food pollutants that urgently need to be treated. A small amount of pesticide residues can affect normal hormones, in...

Claims

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Application Information

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IPC IPC(8): G01N21/76C08J9/26
Inventor 葛慎光于京华黄加栋朱元娜颜梅张丛丛万夫伟葛磊赵佩妮高伟强吴丹张瑾解文秀
Owner UNIV OF JINAN
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