Molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residue and application thereof

Abstract

The invention relates to a molecular imprinting chemiluminescence sensor for detecting trace amount pesticide residues, belonging to the technical field of the detection of the pesticide residues. Theinvention also relates to a method for detecting the trace amount pesticide residues contained in agricultural product samples by using the molecular imprinting chemiluminescence sensor, which comprises the following steps: (1) selecting a functional monomer; (2) uniformly mixing a template molecule, the functional monomer, a cross linker, a pore-forming agent, an initiating agent and a silicon source to prepare an MIPs collosol according to a mole ratio of 0.1-1 to 1-10 to 5-25 to 20-60 to 0.05-0.15 to 20-50; (3) preparing a quantum dot material solution; and (4) modifying the MIPs collosoland quantum dot materials to the surface of a millipore plate to manufacture the chemiluminescence sensor by a layer-layer self-assembly surface modification technique. The invention has the advantages of high specificity and sensitivity, fast detection, low cost, good repeatability and convenient field detection on the detection of the pesticide residues.

Description

technical field [0001] The present invention relates to the technical field of pesticide residue detection, more specifically, a molecularly imprinted chemiluminescent sensor for detecting trace amounts of pesticide residues, and the present invention also relates to the use of the molecularly imprinted chemiluminescent sensor to detect trace amounts in agricultural product samples methods of pesticide residues. Background technique [0002] Pesticide residues mainly include insecticides, fungicides, herbicides, etc. according to different uses of pesticides. Pesticide residues interfere with the normal behavior of organisms and the synthesis, storage, secretion, transport, combination, and clearance of normal hormones related to reproduction and development. [0003] At present, pesticide residues have become a new generation of environmental pollutants and food pollutants that urgently need to be treated. A small amount of pesticide residues can affect normal hormones, in...

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Problems solved by technology

[0006] 1. Uterine weight gain test and uterine calcium-binding protein CaBP-9k mRNA expression detection and protein analysis are often used in in vivo tests. This method is sensitive and specific, but its cost is high and its sensitivity is low, so it is not suitable for large-scale rapid screening ;

[0007] 2. In vitro tests mainly include E-Screen method, receptor competition test, etc. The specificity of the test itself is poor, and false positive results are prone to occur;

[0008] 3. The instrumental detection method for pesticide residues established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the detection of pesticide residues, but this method cannot identify the analyte at one time structure, often need to use GC-MS to confirm the structure, the technical cost is high, the time required by the traditional method is generally long, some even as long as several hours, the operation is complicated, and cannot be used for rapid on-site detection

[0009] 4. For the detection and analysis of pesticide residues, the above methods generally have disadvantages such as low detection sensitivity, high cost, false positive results, complicated detection process, single detection, large amount of reagents, and not suitable for on-site rapid detection. Meet the needs of actual testing

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