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Microfluidic chip for capturing and identifying circulating tumor cells

A microfluidic chip and tumor cell technology, which is applied in laboratory containers, special-purpose bioreactors/fermenters, chemical instruments and methods, etc., can solve the problems of incompetent CTC identification and counting integration, etc. Achieve precise capture and uniform distribution

Active Publication Date: 2017-05-31
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Currently, there is no technology that can integrate CTC capture, identification and enumeration

Method used

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  • Microfluidic chip for capturing and identifying circulating tumor cells
  • Microfluidic chip for capturing and identifying circulating tumor cells
  • Microfluidic chip for capturing and identifying circulating tumor cells

Examples

Experimental program
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Embodiment 1

[0042] Example 1 Microfluidic chip for capturing and identifying circulating tumor cells

[0043] The microfluidic chip provided in this example for capturing and identifying circulating tumor cells includes: a three-layer structure stacked together and sealed to each other, from top to bottom are respectively a microvalve control layer, a microvalve film layer and a microvalve film layer. Substrate with multiple functional areas.

[0044] 1. Microvalve control layer and microvalve film layer

[0045] The microvalve control layer is provided with six holes penetrating the control layer and three gas channels; among them, three holes are sample injection holes, which are connected to the substrate for the inflow and outflow of samples and reagents; the remaining three holes are respectively connected to three gas channels , used to inject gas and control the opening and closing of the microvalve.

[0046] The microvalve film layer is provided with three holes penetrating the ...

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Abstract

The invention provides a microfluidic chip for capturing and identifying circulating tumor cells. The microfluidic chip comprises a three-layer structure sequentially stacked and sealed to each other, wherein the three-layer structure comprises a microvalve control layer, a microvalve film layer and a substrate with multiple functional areas from top to bottom, respectively; the micro-valve control layer is provided with a hole penetrating through the control layer for the sample inflow and outflow, and a gas passage for the gas introduction; the micro-valve film layer can bend to deform under the control of pressure in a thin film layer area corresponding to the gas passage; and the substrate contains three functional areas, respectively an impurity filtration area, a cell separation area and a single cell localization and fluorescence identification area. The three functional areas are connected by three fluid passages, and the opening and closing of each fluid passage are controlled by a separate gas microvalve. According to the invention, the capture, identification and automatic counting of circulating tumor cells in the blood of cancer patients are realized, and an effective means is provided for analysis of the circulating tumor cells.

Description

technical field [0001] The invention relates to the field of microfluidic chip technology and the field of medical diagnosis, in particular to a microfluidic chip for capturing and identifying circulating tumor cells. Background technique [0002] Circulating Tumor Cell (CTC, Circulating Tumor Cell) is a general term for all kinds of tumor cells that exist in the peripheral blood. Most of the CTCs enter the peripheral blood due to spontaneous or therapeutic operations that fall off from solid tumor lesions (primary and metastatic lesions). After apoptosis or phagocytosis, a few can escape and anchor to develop into metastases, increasing the risk of death in patients with malignant tumors. Studies in recent years have shown that the presence or absence and number of CTCs are important indicators of cancer progression and metastasis. Detecting and tracking the number of CTCs in peripheral blood is helpful for early screening, efficacy monitoring, prognosis judgment and recurr...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/34G01N33/574
CPCB01L3/50273B01L3/502753B01L3/502761B01L2200/10B01L2300/0887G01N33/56966G01N33/57492
Inventor 周明行郑晖李仁魏泽文胡志远
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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