Method for inspecting quality of indigowoad leaf Chinese medicament

A quality inspection method and technology of Daqingye, applied in the field of natural medicinal chemistry, can solve the problem of insufficient overall control of the quality of Daqingye

Active Publication Date: 2010-06-16
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
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Problems solved by technology

[0002] Foliage leaf (FOLIUMISATIDIS) is the dried leaf of Isatis indigotica Fort., a cruciferous plant. It has the effects of clearing heat and detoxifying, cooling blood and eliminating spots. Jaundice, dysentery, mumps, sore throat, erysipelas, carbuncle [1] , Modern pharmacodynamic studies have shown that Folium Folium has pharmacological effects such as antibacterial, antiviral, anti-endotoxin, and immune enhancement. [2-6] , at present, the quality control method of Folium Folium is mainly based on the quantitative analysis of components such as indigo and indirubin [7，8] , not enough to control the quality of Folium Folium as a whole, as a commonly used clinical traditional Chinese medicine, its authenticity is affected by many factors such as its place of origin, harvest time, and drying conditions. Therefore, it is necessary to establish a scientific quality The detection method evaluates the quality of Folium Folium, (references: [1] Pharmacopoeia Committee of the People's Republic of China. Pharmacopoeia of the People's Republic of China [M]. Beijing: Chemical Industry Press, 2005: 16. [2] Zheng Jianling, Wang Meihui, Yang Xiuzhen, et al. Study on antibacterial effect of Folium Folium Folium and Radix Radix Radix [J]. Chinese Journal of Microecology, 2003, 15(1): 18-19. [3] Zhang Liantong, Qiu Shicui, Lu Junhua, etc. Study on Antibacterial Effect of Folium Isatidis in Vitro [J]. Shizhen National Medicine, 2002, 23(5): 283-284. [4] Liu Sheng, Chen Wansheng, Qiao Chuanzhuo, et al. Influenza virus effect [J]. Journal of Second Military Medical University, 2000, 21(3): 204-206. [5] Liu Yunhai, Li Qingxiang, Shi Yumei. Anti-endotoxin experiment of Daqingye [J]. Chinese medicinal materials, 1994, 17(6): 36-38 [6] Zhang Shujie, Ma Liren, Gu Dingwei, et al. In vitro study on the regulation of IL-2 and TNF-α secretion by mouse immune cells by water decoction of Daqingye [J]. Shaanxi Traditional Chinese Medicine, 2003, 23(8): 757-759. [7] Sun Lixin, Tang Hong, Yin Ping, etc. Determination of the content of indigo and indirubin in Radix Radix and Folium Isatidis by RP-HPLC [J]. Shenyang Pharmaceutical University Acta Sinica, 2000, 17(3): 191-193. [8] Liu Yingchun, Zhang Ying, Wang Guoqing, etc. RP-HPLC method for the determination of indirubin content in Daqing leaves from different places [J]. Chemical Reagents, 2008, 30(10):759-760.)

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  • Method for inspecting quality of indigowoad leaf Chinese medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of test sample solution

[0020] Using Folium Folium as raw material, weigh 2g of Folium Folium herbal decoction pieces, add 40mL of water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, Filtrate, concentrate under reduced pressure and dry to obtain Folium Folium water extract, add 25mL water to dissolve the obtained Folium Folium water extract, add 25mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, depressurize Concentrated and dried, the residue was dissolved with 50% (V / V) methanol and fixed to 10mL and shaken up, which was the test solution 1; the extracted part was extracted 3 times by adding 25mL water-saturated n-butanol, and combined with water-saturated The n-butanol extract was concentrated under reduced pressure and dried, and the residue was dissolved in 60% (V / V) ethanol and settled to 25mL, which was the test s...

Embodiment 2

[0030] (1) Preparation of test sample solution

[0031] Using Folium Folium as raw material, weigh 4g of Folium Folium herbal decoction pieces, add 80mL of water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, Filtrate, concentrate under reduced pressure and dry to obtain Folium Folium water extract, add 50mL water to dissolve the obtained Folium Folium water extract, add 50mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, depressurize Concentrated and dried, the residue was dissolved with 50% (V / V) methanol and fixed to 10mL and shaken up, which was the test solution 1; the extracted part was extracted 3 times by adding 50mL water-saturated n-butanol, and combined with water-saturated The n-butanol extract was concentrated under reduced pressure and dried, and the residue was dissolved in 60% (V / V) ethanol and settled to 25mL, which was the test s...

Embodiment 3

[0041] (1) Preparation of test sample solution

[0042] Using Folium Folium as raw material, weigh 6g of Folium Folium herbal decoction pieces, add 120mL of water to decoct twice, each time for 1h, filter, combine the filtrate, add ethanol 3 times the volume of the filtrate, stir well, let stand for 24h, Filtrate, concentrate under reduced pressure and dry to obtain Folium Folium water extract, add 50mL water to dissolve the obtained Folium Folium water extract, add 50mL ethyl acetate to extract 3 times respectively, combine the ethyl acetate extracts, depressurize Concentrated and dried, the residue was dissolved with 50% (V / V) methanol and fixed to 10mL and shaken up, which was the test solution 1; the extracted part was extracted 3 times by adding 50mL water-saturated n-butanol, and combined with water-saturated The n-butanol extract was concentrated under reduced pressure and dried, and the residue was dissolved in 60% (V / V) ethanol and settled to 25mL, which was the test ...

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Abstract

The invention provides a method for inspecting quality of indigowoad leaf Chinese medicament, which comprises the following steps: processing indigowoad leaves by sample preprocessing means such as water extraction, ethanol processing, extraction with ethyl acetate and water saturated n-butyl alcohol sequentially, concentration an volume fixation; obtaining a finger-print of the indigowoad leaf Chinese medicament by electron spray mass spectrometry; measuring the nucleoside content and purine content of the indigowoad leaf Chinese medicament by high performance liquid chromatography; and measuring the total flavonoid content of the indigowoad leaf Chinese medicament by UV-VIS spectrophotometry, and integrating the finger-print and the measure data of the content to evaluate the quality of the indigowoad leaf Chinese medicament. The quality inspection method is accurate, quick, convenient and effective.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a quality detection method of the traditional Chinese medicine Folium folium. Background technique [0002] Foliage leaf (FOLIUMISATIDIS) is the dried leaf of Isatis indigotica Fort., a cruciferous plant. It has the effects of clearing heat and detoxifying, cooling blood and eliminating spots. Jaundice, dysentery, mumps, sore throat, erysipelas, carbuncle [1] , Modern pharmacodynamic studies have shown that Folium Folium has pharmacological effects such as antibacterial, antiviral, anti-endotoxin, and immune enhancement. [2-6] , at present, the quality control method of Folium Folium is mainly based on the quantitative analysis of components such as indigo and indirubin [7，8] , not enough to control the quality of Folium Folium as a whole, as a commonly used clinical traditional Chinese medicine, its authenticity is affected by many factors such as its pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/315A61P31/04A61P31/12A61P37/04A61P39/00G01N21/31G01N27/62G01N30/36
Inventor 刘志强闫峻刘舒皮子凤宋凤瑞刘忠英
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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