38 results about "Small molecule metabolism" patented technology

Methods are disclosed related to identifying small molecule metabolites that serve as biomarkers of immune related adverse events (irAEs). In embodiments, treatments are disclosed that reduce the likelihood or prevent irAEs.

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

The invention discloses a substance basis and a detection method for the drug effect of liver-bean jelly, and belongs to the technical field of medicines. In-vitro chemical components of liver-bean jelly are combined to track blood entering components of liver-bean jelly and carry out attribution analysis; obtaining an action target spot of the liver-bean linging for intervening the WD hepatic fibrosis; screening metabolic markers for significant callback of liver-bean linging, and performing enrichment analysis to obtain a significant metabolic pathway; constructing a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network by the action target and the significant callback metabolic marker, and screening a core action target, a core metabolic marker and a core metabolic pathway; a molecular docking technology is used for screening a liver-bean drug effect material basis, and a multi-dimensional network of the liver-bean drug effect material basis, a core action target, a core metabolic marker, a core metabolic pathway and WD liver fibrosis is visualized. The invention discloses a pharmacodynamic material basis and a metabolic regulation mechanism for treating WD hepatic fibrosis by using liver bean paste.

The invention provides an iron-based nano material, a preparation method and application, the material comprises magnetic iron oxide nano particles and a functional modification layer on the surface, the magnetic iron oxide nano particles are Fe3O4, the particle size is 10-30 nm, and the magnetic iron oxide nano particles have specific oxygen vacancy concentration, 337 nm laser absorptivity and saturation magnetization; the functional modification layer is an amino-containing polymer layer, such as a polydopamine or cysteine-polyethyleneimine copolymer, and can reduce salt ion interference. The material solves the problems of low molecular weight region background interference, non-uniform crystallization, low ionization efficiency, poor salt tolerance and the like during detection of 100-400Da small molecular metabolites in trace samples such as tears and the like by optimizing magnetic responsiveness, photo-thermal conversion capability and salt resistance of an existing matrix; the method realizes high sensitivity and high repeatability (detection), and is suitable for metabonomics analysis of trace biological samples.

The invention provides a hematopoietic anti-aging active component extracted from fish embryos as well as an extraction method and application of the hematopoietic anti-aging active component, and belongs to the technical field of active component extraction. The hematopoietic anti-aging active component is extracted from fish embryos which are developed from fish fertilized eggs to the earlier stage of incubation at the optimum temperature and stop development through rapid freezing, and is rich in exosomes, active protein, peptide, chondroitin sulfate, hyaluronic acid, small molecule metabolites, various active factors and the like, and all the nutritional active components are matched with one another, so that the effects of resisting aging and resisting aging are achieved. The cartilage development can be supported, the joint health is ensured, the hematopoietic effect is enhanced, and the skin aging is delayed. The orally disintegrating tablet containing the hematopoietic anti-aging active component can avoid inactivation of bioactive substances caused by high temperature, and the biological activity of the hematopoietic anti-aging active component is reserved to the maximum extent. The hematopoietic anti-aging active component and the orally disintegrating tablet are simple in preparation process and remarkable in hematopoietic anti-aging effect.

The application discloses that 5-oxoproline can be used as a biomarker for diagnosing chemotherapy-induced cardiomyopathy, and for the first time provides a blood metabolic marker for assisting in predicting and early diagnosing chemotherapy drug-induced cardiomyopathy, which has high accuracy and diagnostic reference value. By accurately determining the content of the small molecule metabolic marker 5-oxoproline in blood, the diagnosis of early myocardial injury induced by chemotherapy drugs will no longer completely rely on conventional non-specific myocardial injury markers and imaging methods, but will rely on the content change of metabolic products in the blood of patients as an objective index, thus advancing the time window for discovering subclinical myocardial injury, significantly improving the early diagnosis detection rate, simplifying the diagnosis process, providing an opportunity for early intervention, helping to prevent or reverse the further aggravation of chemotherapy-induced cardiomyopathy, and thus improving the overall survival prognosis of tumor patients.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

Based on the metabolic factors potentially related to reprogramming in oocytes, the present invention lists specific metabolites in oocytes by means of metabolomics and discovers the reductive metabolic characteristics of oocytes. The present invention provides an application of serotonin in promoting cell reprogramming efficiency, cell proliferation and anti-aging, a method for promoting cell rejuvenation and reprogramming, and relates to the field of biotechnology. The present invention proves that including metabolites such as appropriate concentrations of nicotinamide mononucleotide, taurine, serotonin and the oocyte extract itself can promote cell rejuvenation. Among them, especially serotonin, can promote cell reprogramming and cell rejuvenation, extend the lifespan of mice, and reduce the aging level of tissues and organs. Therefore, the present invention finds small molecule metabolites that can promote cell reprogramming and cell rejuvenation through oocytes, which is safer and more reliable than the method independent of gene editing.

The invention discloses a metabolic regulation and control mechanism analysis method for treating WD hepatic fibrosis by using hepatolianling, and belongs to the technical field of medicines, and the method comprises the following steps: performing pharmacological research on components entering blood and a network; the method comprises the following steps: acquiring a metabolic marker of the remarkable callback of the liver-bean lingering on the basis of serum metabonomics, and performing enrichment analysis on the metabolic marker of the remarkable callback of the liver-bean lingering to obtain a remarkable metabolic pathway; a'small molecule metabolite-reaction-enzyme-target 'composite metabolic network is constructed; screening a liver bean drug effect material basis by a molecular docking technology; and a multi-dimensional network of the Ganguoling pharmacodynamic material basis, the core action target, the core metabolic marker, the core metabolic pathway and the WD hepatic fibrosis is visualized. According to the application, 25 drug effect material bases, 11 core action targets, 12 core metabolic markers and 9 core metabolic pathways are obtained.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The invention relates to a preparation for resisting persistent bacteria. The preparation comprises glucose and cephalosporin antibiotics. The invention provides a small molecule metabolite capable of improving the capability of the cephalosporin antibiotics to remove the persistent bacteria, the killing effect of the cephalosporin antibiotics on the persistent bacteria is improved, so that the problem of drug resistance of aeromonas veronii is solved, and the small molecule metabolite can be used as a potential auxiliary drug. Compared with the existing method which only uses antibiotics as antibacterial drugs, the method has better effect, higher safety and operability and better application prospect.

The present invention discloses biomarkers for the severity of ischemic stroke, belonging to the field of biomedicine. The first aspect of the invention provides biomarkers for the severity of ischemic stroke, including protein markers, small molecule metabolite markers, and lipid metabolite markers. The second aspect of the invention provides the use of these biomarkers. The innovation of the biomarkers lies in their ability to provide more accurate diagnostic results, thereby assisting clinicians in better treatment planning. Additionally, these biomarkers offer advantages such as ease of operation and low cost, making them potentially widely applicable in clinical settings.

The invention provides a plant lactobacillus, and also discloses a secondary yellow fermented product obtained by fermenting eucommia ulmoides leaves, rehmannia, dried ginger and dendrobium officinale with the plant lactobacillus as well as a preparation method and application of the secondary yellow fermented product. The sec-yellow leavening can directionally activate a phenylpropane biosynthetic pathway and induce the generation of polyphenol characteristic small molecule metabolites, especially the generation of mulberry furan W. The invention also discloses a preparation method of the sec-yellow leavening. And compared with the raw materials, the fermented product has more active small molecule metabolites. Finally, zebra fish experiments after prednisone modeling prove that the sec-yellow yeast can significantly promote osteogenesis. The sec-yellow leavening is recovered to the normal zebrafish gene expression level mainly by regulating the related gene expression level. Therefore, the sec-yellow leavening can be applied to preparation of foods or medicines for improving bone health.

The present invention discloses a hyperbranched nanocomposite noble metal particle, wherein the hyperbranched nanocomposite noble metal particle is a core-shell structure in which a noble metal is coated with a hyperbranched polyglycidol-n-dodecyl-polyethylene glycol copolymer HPG-C12-PEO, and the noble metal is selected from one or a combination of at least two of palladium, platinum or gold. The present invention also provides a preparation method of the above-mentioned hyperbranched nanocomposite noble metal particle and its application in the detection of small molecule metabolites by laser desorption ionization mass spectrometry. The preparation method provided by the present invention is simple, and the application of the provided hyperbranched nanocomposite noble metal particle in the detection of small molecule metabolites improves the accuracy and sensitivity of the detection.

The invention discloses a preparation method of fruit wine, and particularly aims to solve the problem of mutual exclusion of plant lactobacillus and saccharomyces cerevisiae in low-acid fruit juice fermentation. The method adopts the following core processes: (1) strain combination: selecting plant lactobacillus L14 and saccharomyces cerevisiae NX11424 which are proved to be capable of realizing efficient symbiosis with saccharomyces cerevisiae under a physical isolation condition through screening and realizing deep degradation of malic acid and efficient synthesis of lactic acid; (2) physical isolation: separating the double bacteria through a dialysis membrane with the molecular weight cut-off of 10 kDa, blocking the inhibition effect of the yeast on the plant lactobacillus caused by direct contact of thalli, and allowing free exchange of small molecular metabolites such as organic acids and saccharides; (3) synchronously inoculating, namely simultaneously inoculating the two bacteria into low-acid fruit juice (the initial pH is greater than or equal to 4.5, and the total acid is less than or equal to 3g / L), and fermenting at 25 DEG C in a dark place; the antagonism of mixed bacteria fermentation is relieved, high-activity symbiosis of double bacteria is guaranteed, malic acid degradation and lactic acid efficient synthesis are synchronously realized, the acid feeling balance degree of the fruit wine is remarkably improved, and flavor loss is also avoided.

The application discloses a kind of detection techniques of small molecule metabolite based on enhanced multiple alkali metal ion addition technology, it is related to the field of metabolomics, the detection method of small molecule metabolite, including adding alkali metal cation salt solution to the small molecule metabolite to be measured, then using mass spectrum to detect sample.The detection method disclosed in the application can 1) realize multiple alkali metal cation addition of small molecule metabolite significantly enhanced, significantly improve the detection and quantification performance of small molecule metabolite in laser desorption ionization mass spectrometry, improve small molecule metabolite detection capability;2) realize high-throughput and high repeatability detection of small molecule metabolite in biological fluid;3) and by simple comparison multiple alkali metal cation addition, realize isomer differentiation directly in primary mass spectrum.

The application discloses a combined metabolic marker and detection kit for mitochondrial purity evaluation, the marker comprises phosphatidylcholine PC 34:0 (16:0 / 18:0), sphingomyelin SM 34:1;2O (d18:1;2O / 16:0), sphingomyelin SM 44:2;2O (18:1;2O / 24:1) and hexosylceramide HexCer 42:2;2O (d18:1;2O / 24:1), and is used for judging the new application and kit of mitochondrial purity of mitochondria extracted by a mitochondrial extraction method. The application judges the mitochondrial purity extracted by the method by detecting the relative concentrations of four lipid metabolites in the mitochondrial sample, calculating a combined marker variable P based on a binary logistic regression equation and determining a cut-off value. The kit can realize high-sensitivity and high-efficiency detection of the four small molecule metabolites, and has the characteristics of low detection cost and good repeatability.

The invention discloses an application of Danjie emodin in preparation of a product for inhibiting T cell depletion. It is found through in-vitro screening that the Danjie emodin can significantly inhibit T cell depletion. Mechanism research shows that the Danjie emodin does not influence the secretion of Granzyme B and slightly inhibits the secretion level of a cell factor IFN-gamma; by inhibiting an AKT phosphorylation signal channel, accumulation of protein aggregates in T cells is remarkably reduced, and the cell protein homeostasis is maintained, so that T cell depletion is inhibited. Animal experiments also prove that the T cells treated by the Danjie emodin can significantly inhibit the growth of melanoma after adoptive reinfusion. Therefore, the invention provides the application of the Danjie emodin in preparation of the product for inhibiting T cell depletion, and further provides a new small molecule metabolism intervention strategy for improving the solid tumor immunotherapy effect.

The invention relates to the technical field of feed biology, and discloses a straw protein feed based on fungal fermentation and a preparation method thereof. The feed is prepared by taking a modified straw substrate as a main raw material, performing structure modification on natural straw to improve accessibility of cellulose, introducing an organic small molecule metabolism inducer into a fungus fermentation system, matching a nitrogen source, mineral substances and a fermentation regulation and control aid, and performing fermentation under controlled conditions. The small organic molecule metabolism inducer does not serve as a main carbon source in the fermentation process, but can regulate and control nitrogen metabolism and protein synthesis pathways of fungi and promote efficient conversion from a nitrogen source to microbial protein. Compared with the prior art, the method has the advantages that the crude protein content, the protein digestibility and the nitrogen source utilization efficiency of the straw fermented feed can be remarkably improved, high-value utilization of agricultural straw is realized, and the method is suitable for industrial popularization and application of feed.

The application discloses a new method for high-throughput detection of metabolites based on direct injection high-resolution mass spectrometry, and relates to the fields of detection of small molecule metabolites and high-resolution mass spectrometry. The application is based on direct injection high-resolution mass spectrometry technology, and first, a high-coverage and high-quality-accuracy serum metabolite molecular formula database is constructed by using direct injection Fourier transform ion cyclotron resonance ultra-high-resolution mass spectrometry; second, according to the distribution of the number of molecular formulas in different mass-to-charge ratio ranges in the self-built database, a high-throughput metabolome detection method based on direct injection nanoliter spray ionization high-resolution mass spectrometry is established; finally, non-targeted metabolite data obtained by the high-throughput detection method is searched in the self-built database, and high-throughput molecular annotation of metabolites is realized. The application adopts a two-step acquisition strategy, takes a high-reliability and high-coverage metabolome molecular library as a bridge, and takes into account the detection throughput, coverage and reliability. The method has higher reliability, wider metabolome coverage range and is suitable for large-scale metabolome data analysis.

The invention relates to a method for screening and identifying metabolites and florae with a human immune regulation effect, which comprises the following steps: (1) establishing immune system humanized mice with multiple intestinal types, and analyzing the change of the florae, the metabolites and the immune system through intestinal flora sequencing, serum metabolome sequencing, flow cytometry and transcriptome sequencing; (2) determining the correlation and sequence between flora related metabolites and the change of the immune system through bioinformatics analysis; and (3) confirming the regulation and control relationship between the candidate metabolites and the immune subgroups in the step (2) through biological experiments. By means of the screening method, the chemical small molecule metabolites for regulating and controlling the human immune cells in the normal physiological state can be screened on a large scale, and the chemical small molecule metabolites can be developed into immunotherapy drugs or used as cell therapy drug culture system components. The screened propionic acid can promote cytotoxicity of T cells and CAR-T and secretion of related cytokines in the aspect of function regulation of the T cells, and the metabolite 2-hydroxybutyric acid is highly positively correlated with the Treg cells and can promote generation and functions of the Treg cells.

The present invention discloses a method for preparing cerium oxide micro-nano particles with a multi-level structure, comprising the following steps: Step 1, preparation of a template of 3-aminophenol-formaldehyde spheres; the extended #imgabs0# method is applicable to the preparation of spherical APF resins with uniform particle sizes; Step 2, growing cerium oxide on APF to obtain APF-CeO2; Step 3, removing the APF template to obtain multi-level structured cerium oxide micro-nano particles. The present invention also provides the application of the above-mentioned cerium oxide micro-nano particles in the detection and identification of small molecule metabolites. The detection method of the present invention is non-invasive, has a high detection throughput, high sensitivity, and low cost, meets the clinical requirements for obtaining metabolic fingerprint maps, realizes clinical applications such as diagnosis, prognosis, and biomarker screening, and has great application potential in the in-depth interpretation of the processes of organism growth and development and pathogenic mechanisms at the metabolomics level.

The invention relates to a combined metabolic marker for judging the degranulation inhibition effect of tea-derived polyphenol substances based on rat basophilic leukemia cells and a detection kit thereof. The method comprises the following steps: detecting relative concentrations of lipid metabolites fatty acid (FA22: 5), phosphatidylcholine (PC 38: 2) and phosphatidylcholine (PCO-30: 0) in a rat basophilic leukemia cell sample, calculating a combined marker variable P and a determined intercept point value based on a binary logistic regression equation, and judging the degranulation inhibition effect of the tea-derived polyphenol substance. The kit can realize high-sensitivity and high-efficiency detection of the three small molecule metabolites involved in the invention, and has the characteristics of low detection cost and good repeatability. The combined use of the three small molecule metabolites has a good application prospect.

The application belongs to the biological field, and specifically discloses a fish protein fermentation liquid fertilizer rich in dipeptides and small-molecule organic acids and a preparation method. The fish protein liquid fertilizer prepared by the method is rich in active peptides necessary for plant growth and development, including leucine dipeptide, glutamic acid dipeptide and proline dipeptide, and small-molecule organic acids including lactic acid, phenyl lactic acid and gamma-aminobutyric acid. The composition and content of small-molecule metabolites are regulated by controlling the fermentation time, so as to play the regulating function of fish protein leftovers as non-nutritional elements of liquid fertilizer. The application realizes high-value utilization of fishery waste, accurately regulates the content and composition of metabolites of the prepared liquid fertilizer, and the liquid fertilizer can significantly promote the growth of apple trees, improve the soluble solid content and quality of fruits, and has multiple functions of nutrition, soil improvement and stress resistance enhancement.

The invention relates to the technical field of plant tissue culture, and discloses an azalea tissue culture and rapid propagation method which comprises the following steps: S1, preparing an azalea explant and a culture medium; s2, preparing a dynamic purification culture device and sterilizing; s3, inoculation and dynamic purification culture: inoculating the stem segments into a proliferation culture medium in a growth area in a sterile environment; starting dynamic purification; s4, carrying out subculture multiplication; s5, rooting and seedling hardening. By arranging the dynamic purification culture device, small molecule metabolites generated in a growth area can be continuously removed in the rhododendron tissue culture process, dependence on traditional antioxidants is not needed, the competitive effect of the antioxidants and metabolite adsorption materials is avoided, the stable culture environment can be maintained for a long time, inhibition of metabolite accumulation on plant cell metabolism is reduced, and the survival rate of the rhododendron is increased. The survival of explants and the normal differentiation and growth of bud clusters are effectively guaranteed, and the stability of the tissue culture process and the overall quality of seedlings are improved.

The application provides a MetaSPR optical detection sensor for detecting metabolites in human body fluids, and belongs to the technical field of MetaSPR sensors. The MetaSPR optical detection sensor comprises a MetaSPR chip; the application is characterized in that a metal film with a silver mirror effect is deposited on the back of the MetaSPR chip, and a Ti3C2T x The mixed solution of MXene and graphene oxide is dried to obtain the MGMSPR sensor; when the MGMSPR sensor is used for detecting metabolites in human body fluids, corresponding enzymes and protective layers are added first, then the sample to be detected is added, and the corresponding products are detected by an optical or electrochemical method to realize quantitative analysis. The MGMSPR sensor prepared by the application has a unique nanoscale 3D structure on the surface, has higher sensitivity and specificity, and has a lower detection limit compared with traditional MetaSPR sensors, is suitable for detecting lower concentration samples, and has a wide application potential in the field of small molecule metabolites in human body fluids.

Based on metabolic factors having potential correlation with reprogramming in the oocytes, specific metabolites in the oocytes are listed through a metabonomics method, and reductive metabolism characteristics of the oocytes are found from the specific metabolites. The invention provides application of 5-hydroxytryptamine in promotion of cell reprogramming efficiency, cell proliferation and aging resistance and a method for promoting cell regeneration and reprogramming, and relates to the technical field of biology. The invention proves that the cell regeneration can be promoted by various metabolites including nicotinamide mononucleotide, taurine and 5-hydroxytryptamine with proper concentration and the oocyte extract. Particularly, 5-hydroxytryptamine can promote cell reprogramming and cell regeneration, prolong the life of mice and reduce the aging level of tissues and organs. Therefore, the micromolecule metabolite capable of promoting cell reprogramming and cell regeneration is found through the oocyte, and the method is independent of a gene editing method and is safer and more reliable.

The invention belongs to the technical field of detection, and particularly relates to a preparation method of a test sample for detecting arachidonic acid-like isomers based on magnetic beads, a test sample solution adopts a chemical derivatization reagent system, and covalent coupling magnetic beads are successfully used for nucleic acid and protein separation through surface modification. By accurately designing molecular recognition sites, the chromatographic separation resolution (delta R is larger than or equal to 1.5) of isomer metabolites can be remarkably improved, meanwhile, by means of an isotope double-labeling strategy, the detection sensitivity is improved by 10-100 times, the treatment time of a single sample is shortened to be within 30 minutes, and more importantly, by means of construction of a solid-phase reaction system, the detection sensitivity is greatly improved. Controllable optimization of derivatization reaction efficiency is achieved (the reaction conversion rate is larger than 98%), and a key technical foundation is laid for follow-up development of a full-automatic mass spectrum combined detection technology. The magnetic bead mediated solid phase derivatization strategy is expected to renovate the analysis normal form of small molecule metabolites, and promotes the metabonomics research to develop towards high flux and automation.

The invention discloses a compound microbial preparation as well as a preparation method and application thereof, and relates to the technical field of microbial preparations. According to the invention, lactobacillus johnsonii and clostridium butyricum are utilized to prepare a composite microbial agent, and then the composite microbial agent is combined with a red clover extract and a lotus seed pot extract to prepare the composite microbial preparation. Through a multi-target and multi-mechanism synergistic mechanism, intestinal microecology is improved, intestinal internal barrier is strengthened, inflammation and oxidation are resisted, and the effect of improving intestinal health of broilers is achieved. Wherein the red clover extract and the lotus seed pot extract can create an intestinal environment beneficial to colonization of the compound microbial agent, so that the compound microbial agent can better play metabolism and immune regulation roles; metabolite of the compound microbial agent can also convert part of plant components into micromolecular metabolite with higher bioavailability and higher activity, so that the anti-inflammatory and micro-ecological regulation effects are further enhanced.