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Precious metal core-shell structure nanoparticle as well as preparation and application thereof

A nanoparticle, core-shell structure technology, applied in the field of mass spectrometry detection, can solve the problems of low detection efficiency and interference of the ionization efficiency of the sample to be tested, and achieve the effects of simple operation, less sample consumption, and convenient operation.

Active Publication Date: 2017-06-09
浙江亿纳谱生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the detection of complex biological sample systems, due to the presence of a large number of proteins, salts and other substances, the ionization efficiency of the sample to be tested is seriously disturbed, resulting in low detection efficiency

Method used

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  • Precious metal core-shell structure nanoparticle as well as preparation and application thereof
  • Precious metal core-shell structure nanoparticle as well as preparation and application thereof
  • Precious metal core-shell structure nanoparticle as well as preparation and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0041] The preparation method of the nanoparticle of silicon core silver shell, comprises the following steps:

[0042] Step 1. Using silver nitrate as a precursor, add ammonia water drop by drop until the yellow-brown precipitate in the solution disappears completely, and the solution is clear and transparent, and then constant volume to prepare 10 -2 M ~ 1M silver ammonia solution;

[0043] Step 2, adding the silicon dioxide dispersion liquid with a mass fraction of 2% to 3% to the silver ammonia solution obtained in step 1, and ultrasonicating at room temperature until the liquid is uniformly dispersed;

[0044] Step 3, adding polyvinylpyrrolidone to the mixed solution obtained in step 2, and reacting at 30-100° C. for 0.5-10 hours to form core-shell structures with different thicknesses;

[0045] Step 4. Repeatedly washing the nanoparticles with core-shell structure obtained in step 3 with ethanol and deionized water, and drying them into powder at 60-70° C. to obtain sil...

Embodiment 1

[0051] Embodiment 1 detects glucose standard substance

[0052] Using silicon-core silver-shell nanoparticles as a matrix to perform matrix-assisted laser desorption ionization time-of-flight mass spectrometry on glucose small molecule standards, the steps are as follows:

[0053] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0054] (2) Preparation of silicon-core silver-shell nanoparticles.

[0055] (3) The MALDI target plate was ultrasonically cleaned wit...

Embodiment 2

[0059] Embodiment 2 detects arginine standard substance

[0060] Using silicon-core silver-shell nanoparticles as a matrix to perform matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection steps for arginine small molecule standards are as follows:

[0061] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectrometry signals with a signal-to-noise ratio greater than 10 are used for analysis. Using AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355nm. The pulse electric field delay extraction and reflection working mode is adopted, and the positive ion mode is used for detection. DataExplorer was used to observe, process, and analyze data, and only mass spectrometer signals with a signal-to-noise ratio greater than 10 were used for analysis.

[0062] (2) Preparation of silicon-core silver-shell nanoparticles.

[0063] (3) The MALDI target plate was ultrasonically cle...

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Abstract

The invention discloses a precious metal core-shell structure nanoparticle which is of a double-layer structure, wherein a uniform shell-layer structure is formed by silicon dioxide nanoparticles with uniform granularity as a core which is continuously wrapped with silver nanoparticles; the nanoparticles are spherical granules; the particle size of the nanoparticles is not greater than 1[mu] m; the granularity of the nanoparticles is uniform. The invention further discloses a preparation and application of the nanoparticles in mass spectrometric detection. The precious metal core-shell structure nanoparticle is used in a matrix assistant laser analysis ionization spectrum and is capable of effectively eliminating background noise interference of a conventional organic matrix and improving an analysis ionization effect of a small-molecular substance. Small-molecular metabolin of an actual biological sample system can be effectively recognized and can be precisely quantified, the operation process is simple and convenient, and the detection efficiency can be greatly improved.

Description

technical field [0001] The invention relates to the field of mass spectrometry detection, in particular to a noble metal core-shell nanoparticle and its application in matrix-assisted laser analysis ionization mass spectrometry. Background technique [0002] Since the invention of matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) in the 1980s, as a mass spectrometry technique as an emerging soft ionization method, this technology has successfully solved the difficult problem of non-volatile and thermally unstable biological macromolecules. ionization problem. Due to its high sensitivity, simple and convenient operation, MALDI MS has been widely used in the field of biomacromolecules, especially protein research. -12 mol or even 10 -15 It can accurately measure biomacromolecules with a molecular weight of tens of thousands to hundreds of thousands at the mol level, and mass spectrometric detection of macromolecular protein non-covalent complexes can a...

Claims

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Application Information

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IPC IPC(8): B22F1/02B22F9/24G01N30/00
CPCG01N30/00B22F9/24B22F1/054B22F1/17
Inventor 钱昆黄琳
Owner 浙江亿纳谱生命科技有限公司
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