Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry
An iron oxide and mass spectrometry analysis technology, applied in the field of mass spectrometry detection, can solve the problems of low background noise and good enhancement effect, and achieve the effects of simple operation, good protein resistance and convenient operation
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[0043] The preparation method of iron oxide matrix comprises the following steps:
[0044] The first step: take ethylene glycol as solvent, prepare ferric chloride and trisodium citrate solution;
[0045] The second step: adding sodium acetate to the above solution, and ultrasonicating at room temperature until the solution is homogeneously dispersed;
[0046] The third step: move the mixed solution obtained in the second step to a PTFE-lined stainless steel hydrothermal reactor, and keep the reaction at 200°C for 10 hours to form iron oxide nanoparticles;
[0047] The fourth step: the iron oxide nanoparticles obtained in the third step were repeatedly washed with ethanol and deionized water, and dried at 60° C. into powder.
[0048] Instruments used for characterization:
[0049] The size and morphology characterizations of the products were performed on JEOL JEM-2100F transmission electron microscope (TEM), JEOL JEM-2100F high resolution transmission electron microscope (HRT...
Example Embodiment
[0052] Example 1 Detection of glucose standard
[0053] The steps of matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection of human cerebrospinal fluid using iron oxide nanoparticle matrix are as follows:
[0054] (1) Preparation of instruments and reagents: Laser desorption ionization mass spectrometer, only mass spectral signals with a signal-to-noise ratio greater than 10 are used for analysis. Adopt AB SCIEX TOF / TOF TM 5800 mass spectrometer, Nd:YAG laser, wavelength 355 nm. The working mode of pulse electric field delay extraction and reflection is adopted, and detection is carried out in positive ion mode. Data were observed, processed, and analyzed using DataExplorer, and only mass spectral signals with a signal-to-noise ratio greater than 10 were used for analysis.
[0055] (2) Preparation of iron oxide nanoparticles.
[0056] (3) Composite mass spectrometry analysis of iron oxide nanoparticles and mannitol molecules.
[0057] (4) ...
Example Embodiment
[0061] Example 2 Detection of mannitol standard
[0062] (1) Preparation of instruments and reagents: laser desorption ionization mass spectrometer, only mass spectral signals with a signal-to-noise ratio greater than 10 are used for analysis;
[0063] (2) Preparation of iron oxide nanoparticles;
[0064] (3) Mass spectrometry analysis of iron oxide nanoparticles and mannitol molecules.
[0065] The specific steps are similar to those in Embodiment 1, and are not repeated here.
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