Ferric oxide substrate, preparation and application of ferric oxide substance in cerebrospinal fluid mass spectrometry

An iron oxide and mass spectrometry analysis technology, applied in the field of mass spectrometry detection, can solve the problems of low background noise and good enhancement effect, and achieve the effects of simple operation, good protein resistance and convenient operation
CN106324072AActive Publication Date: 2017-01-11浙江亿纳谱生命科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
浙江亿纳谱生命科技有限公司
Publication Date
2017-01-11

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Abstract

The invention discloses a ferric oxide substrate, preparation and application of the ferric oxide substance in cerebrospinal fluid mass spectrometry. The ferric oxide substrate is a nano spherical particle, the size of the ferric oxide substrate is less than 1mu m, the particle size of the particle is uniform, and the nano spherical particle has a rough surface. The ferric oxide substrate can effectively eliminate background noise interference of a conventional organic substrate, so that the parsing ionization effect of small-molecule substances is greatly enhanced, and extremely high salt tolerance and protein tolerance are achieved. On the basis of the ferric oxide substrate, the invention provides a novel method for accurately quantifying molecules in a cerebrospinal fluid system, therefore, precise identification of the small-molecule substances is realized; and furthermore, the sample amount of cerebrospinal fluid can be reduced to a nano liter level, no organic substrate is added in the process, and no sample pretreatment is needed, so that efficient and quick detection of a low-molecular-weight compound is realized.
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Description

technical field

[0001] The invention relates to the field of mass spectrometry detection, in particular to an iron oxide matrix and its application in cerebrospinal fluid mass spectrometry analysis. Background technique

[0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a new type of soft ionization biological mass spectrometry developed in recent years. Quantitative. MALDI-MS adopts soft ionization technology, and the introduced matrix and sample can form a co-crystal film. Under laser irradiation, the matrix transmits laser energy to the sample molecules to be tested, which can greatly increase the degree of ionization fragmentation of the sample. It can not only be directly applied to the analysis of mixtures, but also can be used to detect whether the sample contains impurities and the molecular weight of impurities. Its accuracy is as high as 0.1% to 0.01%, which is much higher than that of conventional biochemical and high performanc...

Claims

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