54results about How to "Less sample consumption" patented technology

The present invention relates to a high-throughput detection chip for drug resistance gene of bacteria, and an application thereof. The detection chip comprises 117 gene probes, the drug resistance gene probes are selected from 17 categories of drug resistance genes, which comprise extended spectrum beta-lactamase, cephalosporinase, carbapenemase, integrase gene, tetracycline resistance gene, aminoglycoside resistance gene, disinfectant resistance gene, erythromycin resistance gene, macrolide efflux gene, vancomycin resistance gene, multidrug resistance efflux pump gene, mupirocin resistance gene, sulfanilamide resistance gene, tylosin resistance gene, fluoroquinolone resistance gene, chloramphenicol acetyltransferase and commonly-used genetic engineering vector resistance gene. The chip is adopted for detecting the resistance gene of the pathogenic bacteria. The chip is characterized in that: the chip comprises (1) 117-oligonucleotide probe composition and quality control probes of 17 categories of the drug resistance genes; (2) probe arrays, wherein the oligonucleotide probes are solidified on the vector material through arm molecules to form the probe arrays.

The present invention relates to a gene chip for detecting several infections lideases and its application. It can be used for detecting, typing and strain-identifying 7 main infections diseases of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, schistosomiasis, malaria, cholera and hemorrhagic enteritis due to 0157:H7. Said invention can select specific gene probe and PCR primer respectively from S gene of epidemic hemorrhagic fever virus, 56KD protein gene of oriental rickettsia, 23 SRNA gene of leptospirosis, 5D antigen gene of schistosomiasis, SSrRNA gene of malarial parasite, 0157 antigen gene of colibacillus 0157:H7, H7 antigen gene and toxic gene and outer membrane OWP protein gene of cholera vibrio and toxic gene.

An electrophoresis apparatus which is composed of laser, high speed DC motor, rotary coder, high voltage power supply, capillary, buffer solution, reflector and detector is arranged as the follows, capillary is laid out in round shape of symmetric array, high speed DC motor is applied to quicken data collection speed and rotary coder is applied to confirm position of each capillary accurately, rotary reflector is set on central axle of round column on capillary, its reflection face and central axle are presented as of 45 degree angle and laser is focused on capillary, fluorescent signal excited on capillary is transmitted to detector along with light path.

The invention relates to a biological engineering detection product and an application of the gene chip, and in particular relates to a detection gene chip for helicobacter pylori infection individualized treatment. The chip is capable of simultaneously detecting polymorphisms of helicobacter pylori clarithromycin medicine-resistant mutation sites A2142G and A2143G, carbostyril medicine-resistant mutation sites Asn87(N87K) and Asp91(D91G/Y/N), and sites 2C19*2(G6981A) and 2C19*3(G636A) related to metabolism of a proton pump inhibitor on human cytochrome enzyme CYP450. The chip detection method disclosed by the invention is rapid and accurate and is capable of identifying helicobacter pylori etiology, carrying out medicine-resistant analysis of clarithromycin and carbostyril, and analyzing metabolic individual differences of the proton pump inhibitor, so that the detection gene chip is used for guiding individualized administration of helicobacter pylori infection triplex process treatment.

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the lower shell; the upper and middle filter paper chips are bonded through the double-sided tape; the lower filter paper chip slides on the water-based slide frame relative to the upper and middle filter paper chips, so that the different reaction reagents can sequentially flow among the multiple layers of filter paper chips. According to the test card, an early diagnosis device applied in clinical site is provided for patients.

The invention relates to a method for designing and preparing an electrochemical detection-microfluidic multichannel chip based on a self-assembled monolayer technology. The method provided by the invention realizes designing and preparation of the electrochemical detection-microfluidic multichannel chip, utilizes the combination of the self-assembled monolayer technology and electrochemical detection in the field of the microfluidic, and realizes high-efficiency low-consumption detection of multiple substances. The method provided by the invention comprises the following steps of 1, designing and preparing electrode templates and microchannel templates corresponding to the electrode templates, 2, through microfluidic channels, fixing chemical molecules or biomolecules on the surfaces of microelectrodes through the self-assembled monolayer technology so that self-assembled layers are formed and modify the microelectrodes, 3, feeding an analyte through the microfluidic channels so that the analyte contacts with the modified microelectrodes and undergoes a reaction, and 4, feeding a test solution through the microfluidic channels and carrying out detection of the modified microelectrodes by an electrochemical method. The electrochemical detection-microfluidic multichannel chip obtained by the method has multiple electrode systems. The templates of the electrochemical detection-microfluidic multichannel chip can be prepared according to demands. The method provided by the invention can realize self-assembly and electrochemical detection of a single electrode or multi-electrodes.

The invention relates to a method for rapidly and quantitatively evaluating spaceflight fuel performances, and relates to a method for rapidly detecting a main curve of tensile strength for unidirectional stretching of a solid propellant. The method comprises eight steps of: analyzing a relation between a dynamic energy storage modulus and a setting, calculating a relation between a stress relax modulus and a setting temperature, drafting a difference graph of a main curve of the dynamic energy storage modulus to a main curve of the stress relax modulus, detecting tensile strength of unidirectional stretching, obtaining a dynamic frequency value, obtaining a dynamic energy storage modulus value, obtaining a tensile strength value of unidirectional stretching, and drafting a main curve map of tensile strength of unidirectional stretching. By detecting the main curve of the dynamic energy storage modulus and the main curve of the stress relax modulus of the solid propellant, and based on a relative formula, the dynamic tensile strength value and the main curve of the tensile strength of the unidirectional stretching are obtained at any temperature and any stretching speed. The method is widely used for rapidly detecting fuel performances of the solid propellant, and has advantages of short detection process, low consumption sample, low test cost and good data repeatability.

The invention relates to a co-detecting method for environmental estrogens in a surface water body, and aims at solving the problems that the treatment process time is long, a large quantity of samples and reagents are consumed in an off-line solid phase extraction method, the process is complicated, the interference factors are more, and loss is large. The co-detecting method is implemented through the following steps that 1, sample collecting and pretreating are performed; 2, filtering and enriching are performed, wherein a to-be-detected surface water body sample which is preserved after being pretreated are enriched and purified through an on-line solid phase extraction device column; 3, detecting and analyzing are performed, wherein a result obtained in the step 2 is detected by adopting an ultra-high performance liquid chromatography tandem mass spectrometer to obtain the detection limit and a quantitative and qualitative result of the seven environmental estrogens in the to-be-detected surface water body sample; 4, a standard curve is obtained; 5, the result obtained in the step 3 is compared with the standard curve to obtain the content and recovery rate of the environmental estrogens. The co-detecting method is applied to the field of surface water detection.

This invention discloses one test method with drug fat package rate, which comprises the following steps: adding the liquid not reacting with resin double molecule layer into resin mixture hanging liquid to achieve balance through dialysis water concentration to get outer phase for fat test; this invention adds double molecule layer without reaction into sample to be tested; filtering to get property liquid to test its medicine and liquid concentration to compute discrete bus to pack rate.

The invention discloses a visual measurement method for uric acid based on a mobile reaction interface and an electrophoretic titration chip. The method comprises the following steps of: firstly, injecting a gel mixed with a background buffer solution and sodium acetate into a channel of the electrophoretic titration chip, and filling the background buffer solution in a cathode chamber; then injecting (1) urine to be tested or (2) a mixture of serum and background buffer solution into an anode chamber; adding uricase, and then adding colorless crystal violet and horseradish peroxidase in the anode chamber after the reaction is sufficient, wherein the colorless crystal violet and the horseradish peroxidase react with the reaction product in the original anode chamber to produce colored crystal violet ions; electrophoresing the colored crystal violet ions into the channel and reacting with sodium acetate in the channel to produce a titration neutralization reaction interface; and calculating a linear relationship between a moving distance of a moving reaction interface and the uric acid detecting sample with the known concentration to obtain a contrast curve, thereby calculating theuric acid concentration of the sample to be tested. According to the visual measurement method for uric acid based on the mobile reaction interface and the electrophoretic titration chip, the contentof uric acid in the sample to be tested can be rapidly and effectively determined, the time and sample consumption is low, the portable performance is excellent, and the detection in different environments can be realized.

The invention discloses a paper porosity measuring and pore diameter analyzing method based on digital image processing. According to the method, a paper cellulose fiber microstructure image is converted into a gray level image, a binary image is obtained through a double-threshold binarization method, morphology is utilized to optimize the binary image, an improved fiber structure binary image isobtained, equivalent circle diameters of all pore areas can be further obtained, and finally, pore diameter distribution statistics are performed according to pixel areas, actual areas and the equivalent circle diameters of all the pore areas.

The invention discloses a ferric oxide substrate, preparation and application of the ferric oxide substance in cerebrospinal fluid mass spectrometry. The ferric oxide substrate is a nano spherical particle, the size of the ferric oxide substrate is less than 1mu m, the particle size of the particle is uniform, and the nano spherical particle has a rough surface. The ferric oxide substrate can effectively eliminate background noise interference of a conventional organic substrate, so that the parsing ionization effect of small-molecule substances is greatly enhanced, and extremely high salt tolerance and protein tolerance are achieved. On the basis of the ferric oxide substrate, the invention provides a novel method for accurately quantifying molecules in a cerebrospinal fluid system, therefore, precise identification of the small-molecule substances is realized; and furthermore, the sample amount of cerebrospinal fluid can be reduced to a nano liter level, no organic substrate is added in the process, and no sample pretreatment is needed, so that efficient and quick detection of a low-molecular-weight compound is realized.

The invention discloses a method for rapidly, sensitively and quantitatively detecting glucose in serum and belongs to the field of biochemical reagent detection. The method comprises the following steps: firstly removing protein in the serum by adopting an organic solvent, carrying out chemical labeling by virtue of a boric acid group and a quaternary ammonium group which are contained in a structure, then adopting black phosphorus-assisted laser desorption ionization-flight time mass spectrometry, and then realizing quantitative detection on the glucose in the serum. The method disclosed by the invention is simple and rapid in operation, and the whole experimental procedure can be completed within 5 minutes; sample consumption amount is low, and endogenous glucose detection can be realized in 0.5 microliter of the serum only; and result is accurate, and large-scale sample determination requirement is met.

The invention discloses an optical fiber micro-air cavity photoacoustic cell, a preparation method and a dissolved gas detection method. The optical fiber micro-air cavity photoacoustic cell comprisesan optical fiber microstructure and a micro-air cavity located on the end face of the microstructure, the optical fiber microstructure is formed by welding a section of capillary tube to one end of asingle-mode optical fiber, and the end face of the single-mode optical fiber is plated with a gold film; and the other end of the single-mode optical fiber is introduced into the laser heating gold film to vaporize surrounding liquid to form the optical fiber micro-air-cavity photoacoustic cell. The dissolved gas in the liquid diffuses into the photoacoustic cell under the driving of the solubility gradient, absorbs pump light injected into the photoacoustic cell through the single-mode optical fiber, generates a photoacoustic signal and causes gas-liquid interface deformation of the micro-gas cavity; the deformation is detected by another beam of detection light injected by the optical fiber, and the concentration of the dissolved gas to be detected is reduced by combining the gas absorption coefficient. The optical fiber micro-air-cavity photoacoustic cell is simple to prepare, low in cost and small in size, and can be used for in-situ and rapid dissolved gas detection.

The invention discloses a method for testing a bisimide vulcanizing agent. A structure of the bisimide vulcanizing agent is as shown in a formula (I). According to the testing method, the reaction exothermal condition of a bisimide compound and sulphur under the action of a vulcanization activator zinc oxide, stearic acid and a vulcanization accelerator is tested by differential scanning calorimetry. By comparing the highest exothermal temperatures of reaction exothermal peaks and peak areas of the exothermal peaks, reactivity and reaction degrees of the bisimide vulcanizing agents with different structures can be distinguished, and anti-reversion behaviors of the substances after rubber reversion can be further speculated. With reference to the chemical formula in the description, R1 is selected from straight-chain alkylidene of which the carbon atom number is 1 to 10, alkylidene with a branched chain, of which the carbon atom number is 2 to 10, and alkaryl or aralkyl of which the carbon atom number is 7 to 20; R2, R3, R4 and R5 are the same or different, and are respectively and independently selected from one of hydrogen, carboxyl, aldehyde, alkyl with a straight chain or a branched chain, of which the carbon atom number is 1 to 5, and aryl of which the carbon atom number is 6 to 20.

A micro-chamber microfluidic system for microbial growth image detection is disclosed. The system includes a microscope, a constant current syringe pump, a syringe, a constant-temperature stage, a micro-chamber microfluidic chip, a collector and a CMOS video detection component. The constant-temperature stage is disposed on a microscope stage. The micro-chamber microfluidic chip is arranged on theconstant-temperature stage. A micro-chamber structure having an independent design can be used for real-time microbial culture. The CMOS video detection component is connected and fixed above the microscope, and is used for measuring morphological changes of bacteria in each micro-chamber in the micro-chamber microfluidic chip. The CMOS video detection component also includes a computation unit,and the computation unit is used for acquiring clear gray level image information of microbes based on a linear spatial filtering implementing principle and a corresponding segmentation equation and coefficients. The system effectively overcomes a technical defect that traditional microbial image detection methods are time-consuming, labor-consuming, expensive, single in function, and incapable ofacquiring long-time real-time real microbial growth image information, and has a great application prospect.

The invention discloses a multi-pulse free decay method used for signal acquisition of a microwave spectrometer. As life of a gas molecular beam to be measured which is injected into a vacuum chamberis at the millisecond level, single-time microwave excitation and sampling process of free induction decay signals are compared. A sample gas is excited and sampled for multiple times such that signalsampling efficiency is raised by nearly one order of magnitude; and sample consumption also can be reduced, and experimental cost is saved. If a nozzle switch is delayed, background noise also can beeliminated.

The invention relates to a droplet digital PCR chip, a detection system, a detection method and a manufacturing method. The droplet digital PCR chip comprises a chip body, wherein the chip body is provided with a liquid injection pool, a droplet generation pool, a flow pool, a droplet storage pool and a first driving source, the liquid injection pool, the droplet generation pool, the flow pool andthe droplet storage pool are sequentially communicated, the first driving source is communicated with the liquid injection pool, and the bottom of the liquid injection pool comprises a diaphragm; thedroplet generation pool is provided with a droplet generation cavity; a sample injection opening is formed in one end of the liquid injection pool, the other end of the liquid injection pool extendsinto the droplet generation cavity, and a first capillary pipeline is formed in a communicating path of the liquid injection pool and the droplet generation cavity; and the first driving source can drive the diaphragm to elastically expand and contract so as to extrude liquid in the liquid injection pool out of the droplet generation cavity through the first capillary pipeline to form droplets. The chip can be directly subjected to PCR, secondary transfer of the droplets is not needed, the state of the droplets is better maintained, PCR is facilitated, the detection efficiency and accuracy areimproved, and the chip has a wide application prospect in droplet digital PCR devices.