Co-detecting method for environmental estrogens in surface water body

A technology of environmental estrogen and detection method, which is applied in the field of co-detection of environmental estrogen in surface water bodies, can solve the problems of large sample and reagent consumption, long processing time, and many interference factors, so as to reduce the sample volume and workload. , Improve the accuracy of qualitative and quantitative, reduce the effect of volume

Inactive Publication Date: 2016-02-17
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of long processing time, offline solid-phase extraction method consumes a large number of samples and reagents, complex process, many interference factors, and large losses, and proposes a co-detection of environmental estrogen in surface water method

Method used

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  • Co-detecting method for environmental estrogens in surface water body
  • Co-detecting method for environmental estrogens in surface water body
  • Co-detecting method for environmental estrogens in surface water body

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Experimental program
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specific Embodiment approach 1

[0039] Specific implementation mode one: combine figure 1 Describe this embodiment, the co-detection method of environmental estrogen in a kind of surface water body of this embodiment, specifically is prepared according to the following steps:

[0040] Step 1. Sample collection and pretreatment: Measure the raw water sample of the surface water body, acidify it with hydrochloric acid, add an internal standard substance to obtain the surface water body sample to be tested, and store the surface water body sample to be tested in a sealed environment at 3-4 °C, and Test within 48 hours;

[0041] Step 2. Filtration and enrichment: filter the preserved surface water samples to be detected through a glass fiber filter membrane, and the filtered surface water samples to be detected are injected into the column of an online solid-phase extraction device by an automatic sampler Phase extraction device column for enrichment and purification;

[0042] Step 3, detection and analysis: ...

specific Embodiment approach 2

[0048]Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: sample collection and pretreatment in the step 1: take the raw water sample of the surface water body, acidify it with hydrochloric acid, add an internal standard substance, and obtain the surface water body to be tested For samples, seal the surface water samples to be tested and store them in an environment of 3-4°C, and test them within 48 hours; the specific process is as follows:

[0049] Measure 500mL of surface water raw water sample, adjust pH=4 with 5mol / L hydrochloric acid, add internal standard substance BPA-d16 to obtain the surface water sample to be tested, and store the surface water sample to be tested in a sealed environment at 3-4°C. and tested within two days.

[0050] Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0051] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the addition of internal standard substance BPA-d 16 20 μg.

[0052] Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a co-detecting method for environmental estrogens in a surface water body, and aims at solving the problems that the treatment process time is long, a large quantity of samples and reagents are consumed in an off-line solid phase extraction method, the process is complicated, the interference factors are more, and loss is large. The co-detecting method is implemented through the following steps that 1, sample collecting and pretreating are performed; 2, filtering and enriching are performed, wherein a to-be-detected surface water body sample which is preserved after being pretreated are enriched and purified through an on-line solid phase extraction device column; 3, detecting and analyzing are performed, wherein a result obtained in the step 2 is detected by adopting an ultra-high performance liquid chromatography tandem mass spectrometer to obtain the detection limit and a quantitative and qualitative result of the seven environmental estrogens in the to-be-detected surface water body sample; 4, a standard curve is obtained; 5, the result obtained in the step 3 is compared with the standard curve to obtain the content and recovery rate of the environmental estrogens. The co-detecting method is applied to the field of surface water detection.

Description

technical field [0001] The invention relates to a co-detection method for environmental estrogen in surface water bodies. Background technique [0002] Environmental estrogens (EEs) refer to a class of compounds that interfere with the metabolism of normal endocrine substances and disrupt the stability of the body after entering the body, including polychlorinated biphenyls, dioxins, bisphenols and alkylphenols, and phthalic acid Compounds such as esters and steroids. The existence of these substances in extremely small amounts in the environment may cause potential harm to the ecosystem and human health. Such substances can produce endocrine disrupting effects at extremely low concentrations (ng / L level or below), thereby having potential adverse effects on the health and reproduction of humans, domestic animals, and wild animals. The natural steroid estrogens estrone (E1), 17p-estradiol (E2), estriol (E3) secreted by human and animal ovaries, and the synthetic estrogen 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08
CPCG01N30/08G01N30/88
Inventor 陈忠林尹航靳倩倩
Owner HARBIN INST OF TECH
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