Determination method for entrapment efficiency of liposome

A measurement method and liposome technology, applied in the direction of testing pharmaceutical preparations, special data processing applications, instruments, etc., can solve the problems of long time consumption, large error in results, and high requirements for instruments, and achieve short time consumption, good accuracy, and experimental results. low cost effect

Inactive Publication Date: 2011-08-31
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has high requirements for instruments, takes a long time, and has the disadvantages of large error in the results mentioned above.

Method used

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  • Determination method for entrapment efficiency of liposome
  • Determination method for entrapment efficiency of liposome
  • Determination method for entrapment efficiency of liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 (determination of the encapsulation efficiency of the entire sennecline liposome by the reverse dialysis method of measuring the volume of the external aqueous phase with an auxiliary substance).

[0029] 1.1 Preparation of entire sennecline liposome samples

[0030] The whole-edge sennecline (A base) liposomes were prepared by dry film dispersion method. Take 0.2g of base drug A, 2g of injection-grade soybean lecithin, 0.5g of cholesterol, and 0.1g of vitamin E in a 500ml eggplant-shaped bottle, add 50ml of chloroform to dissolve, evaporate the chloroform under reduced pressure at 60°C and 50r / min to form Lipid dry film, vacuum-dried at room temperature for 12 hours to remove residual chloroform, hydrated the dry film with 50ml CBS 4.0, filled with nitrogen, shaken on the shaker to dissolve the film on the bottle wall, and then stood it in the refrigerator at 4°C for 12 hours, and finally used High-shear disperser disperses to make the whole system unifor...

Embodiment 2

[0065] Embodiment 2 (determination of the encapsulation efficiency of liposome by the centrifugation ultrafiltration method of measuring external aqueous phase volume with auxiliary substance)

[0066] 2.1 Effect of ultrafiltration on the concentration of free drug solution

[0067] Take three different concentrations (1.22, 0.50, 0.10 mg / ml respectively) of A alkali aqueous solution in three parts, each part is 200 μL, and carry out ultrafiltration (Ultrafiltration unit adopts Microcon YM 100K, Millipore), and measure the filtration by HPLC respectively. The peak area corresponding to the base A in the solution and the solution before ultrafiltration. The results are shown in Table 5. It can be seen that the ultrafiltration membrane has a small amount of adsorption on the drug. When the drug concentration is large enough, it can be considered that the ultrafiltration has no effect on the concentration of the drug solution.

[0068] Table 5 The influence of ultrafiltration on...

Embodiment 3

[0080] Example 3 (determination of the encapsulation efficiency of the entire sennecline liposome by the reverse dialysis method of measuring the volume of the external aqueous phase with an auxiliary substance).

[0081] The test procedure and test conditions are the same as in Example 1, with phenol red replacing 5-Fu, and the test results are the same as in Example 1.

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PUM

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Abstract

This invention discloses one test method with drug fat package rate, which comprises the following steps: adding the liquid not reacting with resin double molecule layer into resin mixture hanging liquid to achieve balance through dialysis water concentration to get outer phase for fat test; this invention adds double molecule layer without reaction into sample to be tested; filtering to get property liquid to test its medicine and liquid concentration to compute discrete bus to pack rate.

Description

technical field [0001] The invention relates to a method for measuring the drug encapsulation efficiency of a drug-containing liposome. Background technique [0002] Liposomes are closed vesicles with a bilayer structure made of phospholipids and (or without) additives as the skeleton membrane material. There are two long hydrophobic hydrocarbon chains and a hydrophilic group in the common phospholipid molecular structure. When an appropriate amount of phospholipid is added to water or buffer solution, the phospholipid molecules are aligned, and the hydrophilic groups face the water phase on both sides. , The hydrophobic hydrocarbon chains are relatively associated with each other and form a bilayer to form a liposome. The phospholipids used to prepare liposomes include natural phospholipids, such as soybean phospholipids, egg yolk phospholipids, etc.; synthetic phospholipids, such as dipalmitoylphosphatidylcholine, distearoylphosphatidylcholine, etc. A commonly used addit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15G06F19/00
Inventor 郑杭生徐莲英陈建明
Owner SHANGHAI UNIV OF T C M
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