14 results about "Natural phospholipids" patented technology

The present application relates to a kind of dihydrocarbylimidazole biomimetic lipid compound and its preparation method and application, dihydrocarbylimidazole biomimetic lipid compound structure imitates natural phospholipid design, modification is not less than 10 carbon atoms alkyl on the 4th and 5th of imidazole, and modification is different alkyl chain length primary amine on 2nd position;Dihydrocarbylimidazole biomimetic lipid compound is mixed with therapeutic drug and other adjuvant, can be prepared to be loaded in the lipid nanoparticle of therapeutic drug, can be used as drug delivery system for the preparation of brain-targeted drug.Dihydrocarbylimidazole biomimetic lipid has the function of dynamic control blood-brain barrier, can realize the reversible opening of blood-brain barrier;Formed drug-loaded lipid composition can cross blood-brain barrier and better realize the brain-targeted delivery of loaded therapeutic drug;Drug-loaded lipid composition consisting of dihydrocarbylimidazole biomimetic lipid belongs to a new generation of biomimetic nanomedicine carrier, provides new strategy for realizing the brain-targeted delivery of different kinds of drugs.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a polypeptide in-situ gel long-acting injection as well as a preparation method and application thereof, the injection is prepared from the following components: a polypeptide substance, a gel matrix and a biocompatible solvent, the polypeptide substance is tetrapeptide H-Tyr-D-Arg-Ph-Gly-NH2, and the mass volume percent of the polypeptide substance is 0.1%-4% (w / v); the gel matrix is an amphiphilic compound and natural phospholipid, and the mass ratio of the amphiphilic compound to the natural phospholipid is (0.7-7): 1; the biocompatible solvent is 1, 2-propylene glycol, and the mass fraction of the 1, 2-propylene glycol is 15%-30%. The injection has good fluidity and low viscosity, meets the viscosity requirement of a pharmacopoeia injection, is in contact with body fluid after being injected, is subjected to a solvent exchange reaction and is converted into hexagonal phase liquid crystal in-situ gel to form a medicine storage cavern, so that the release speed of tetrapeptide from the in-situ gel can meet the long-acting pain relieving requirement.

The invention discloses a novel application of emodin and a high-density lipoprotein analogue in preparation of a medicine. The high-density lipoprotein analogue comprises apolipoprotein A-1 single mimetic peptide and natural phospholipid. The invention has the advantages of long-term stability and no immunogenicity and organ toxicity risk, thereby providing a clinically convertible solution for precise treatment of nerve injury.

The invention discloses a method for producing phosphatidylserine through enzyme catalysis, and belongs to the technical field of biology, and the method specifically comprises the following steps: in a reaction system of a [BMIM] [BF4] aqueous solution, taking natural phospholipid containing phosphatidylcholine and cephalin as a raw material, adding L-serine, calcium salt and phospholipase D, and carrying out a trans-acylation reaction. By introducing cephalin (4%-10%, preferably 6%-8%) and sphingomyelin (5%-10%) in a specific proportion and combining with a [BMIM] [BF4] ionic liquid system, high efficiency and high purity (the purity gt, 79.6% and the yield gt, 76.4%) of PS production are realized, and the problem that the yield and the purity cannot be considered at the same time in the traditional process is thoroughly solved.

The invention provides a high-activity Chinese yam instant tablet and a preparation method thereof. Comprising the following steps: (1) raw material pretreatment: washing, peeling and slicing fresh Chinese yam, protecting color, drying, crushing, and carrying out airflow superfine grinding to obtain Chinese yam powder with high purity and uniform granularity; (3) carrying out fine treatment by adopting a colloid mill to obtain Chinese yam enzymolysis slurry, adding Chinese yam source natural phospholipid, and stirring; and (4) carrying out gradient temperature treatment. The high-quality instant Chinese yam product which is excellent in solubility, high in nutrition retention rate, pure and pleasant in flavor, fine and smooth in taste and good in storage stability is successfully prepared by constructing a multi-dimensional synergistic technical system of superfine grinding, synergistic enzymolysis, flavor regulation and control and gradient gelatinization shaping; and the quality is improved from'brewing 'to'extremely instant' and from'common food 'to'high-activity functional food'.

The invention relates to the technical field of seasoning processing, and discloses a cooking freshness-locking process for preparing green pepper sauce, which comprises the following steps: carrying out graded ultrasonic wall breaking treatment on green pepper pulp, promoting chlorophyll release by low-power ultrasound in the first stage and keeping fiber bundles complete, and thoroughly extracting chlorophyll by enhanced ultrasound in the second stage; separating the fiber bundles softened by enzymolysis from the pulp; preparing a natural phospholipid stabilized plant essential oil microemulsion, a modified polysaccharide-protein graft compound emulsifier and a natural chelated polysaccharide compound; performing low-temperature stir-frying for alkaline color protection and essential oil oxidation resistance, performing medium-temperature cooking to form polysaccharide gel embedded chlorophyll, and performing rapid cooling to 70-75 DEG C to lock a color protection state; soluble dietary fibers, xanthan gum-guar gum and konjac glucomannan are added to regulate the texture, and fiber bundles are added again to reconstruct tissues; and carrying out hot filling and sealing at 70-75 DEG C. According to the invention, high-efficiency release and long-acting protection of chlorophyll are realized, and good granular sensation and flowability of the green pepper sauce are maintained.

The application discloses an enzyme catalysis production method of phosphatidylserine, and belongs to the technical field of biology, and specifically comprises the following steps: in a reaction system of [BMIM][BF4] aqueous solution, taking natural phosphatide containing phosphatidylcholine and cephalin as raw materials, adding L-serine, calcium salt and phospholipase D to carry out a transacylation reaction. By introducing specific proportions of cephalin (4%-10%, preferably 6%-8%) and sphingomyelin (5%-10%), in combination with the [BMIM][BF4] ionic liquid system, the application realizes efficient and high-purity (purity>79.6%, yield>76.4%) production of PS, and completely solves the problem that yield and purity cannot be considered simultaneously in the traditional process.

This invention relates to an enzyme-responsive brain-targeting sustained-release formulation based on dialkylimidazolium biomimetic lipids, its preparation method, and its application, belonging to the field of drug delivery. First, dialkylimidazolium biomimetic lipids are covalently coupled with natural phospholipids to form a dialkylimidazolium conjugate. Then, this dialkylimidazolium conjugate is self-assembled with dialkylimidazolium biomimetic lipids, natural phospholipids, cholesterol, and a therapeutic drug to form a drug-loaded lipid nanoparticle delivery system. This drug delivery system can effectively cross the blood-brain barrier to achieve brain-targeted delivery of the loaded drug. In the glioma microenvironment, this drug delivery system can gradually hydrolyze the dialkylimidazolium conjugate through an enzyme-responsive mechanism to achieve sustained release of the loaded drug, providing a new strategy for improving the brain targeting and efficacy of different types of anti-glioma drugs, such as small nucleic acid drugs, peptide drugs, and small molecule drugs.

The application discloses phospholipid and a preparation method and application thereof, and relates to the technical field of organic compounds. The phospholipid of the application takes lysophosphatidylcholine, p-carboxymethyl formaldehyde and a fatty amine as raw materials, and the synthesized phospholipid has a dynamic covalent bond Schiff base structure. The molecular structure of the phospholipid can realize dissociation and assembly under the action of acid or alkali. Compared with conventional natural phospholipids, the problems such as complex composition and strong odor are avoided, the phospholipid can be used for constructing active ingredient liposomes of pesticides and cosmetics, the formed liposomes have excellent stability and transdermal absorption performance, and the Schiff base dynamic covalent bond structure in the structure of the phospholipid can also realize the slow and controlled release of the pesticide raw drug, improve the half-life of the pesticide, and play a role of reducing application and increasing efficiency.

The invention relates to the technical field of traditional Chinese medicine preparations and health-care foods, and discloses healthy-energy-strengthening and anti-aging tablet candies taking gastrodia elata as a monarch drug and a preparation method of the tablet candies, and the tablet candies are prepared from superfine ground gastrodia elata powder, a ginseng extract, natural phospholipid, divalent metal cation salt and auxiliary materials. The core of the preparation method is that natural phospholipid, ginseng extract and divalent metal salt are prepared into functional wetting emulsion, active and flavor substances in gastrodia elata are induced to be subjected to in-situ self-assembly in the wet granulation process, and a supramolecular association body anchored by an ion bridge is formed; and curing the structure through a double-solvent gradient volatilization drying process. The gastrodia elata preparation solves the common problems of significant bad flavor, unstable chemical properties and low bioavailability of gastrodia elata preparations. The obtained tablet candy is good in taste masking effect and high in chemical stability, and the dissolution efficiency and the transmembrane permeation efficiency of the active ingredients are remarkably improved.

The invention relates to an enzyme response type brain-targeted sustained release preparation based on dialkyl imidazole bionic lipid as well as a preparation method and application of the enzyme response type brain-targeted sustained release preparation, and belongs to the field of drug delivery. The preparation method comprises the following steps: covalently coupling dialkyl imidazole bionic lipid and natural phospholipid to form a diester conjugate; then, the diester conjugate, dialkyl imidazole bionic lipid, natural phospholipid, cholesterol and a therapeutic drug are self-assembled to form a drug-loaded lipid nanoparticle delivery system; the drug delivery system can effectively pass through a blood brain barrier to realize brain-targeted delivery of loaded drugs; the drug delivery system can gradually hydrolyze the dilipid conjugate through an enzyme response mechanism in a brain glioma microenvironment, realizes slow release of the loaded drug, and provides a new strategy for improving brain targeting and curative effects of different types of anti-brain glioma drugs such as small nucleic acid drugs, polypeptide drugs and small molecule drugs.

The invention relates to the technical field of biological medicines and nano preparations, in particular to a preparation method and application of a ginseng-sourced nano-like particle loaded medicine. The method comprises the following steps: S1, extracting a ginseng exosome; s2, purifying the ginseng exosome; and S3, preparing the drug-loaded nanoparticles. According to the invention, GENs is taken as an oral carrier, the problem of low bioavailability of free drugs is solved, the drug stability is enhanced by a natural phospholipid double-layer structure, and intestinal absorption and liver targeting accumulation are facilitated. The dual effects of the active ingredient of the GENs and the loaded drug are obviously better than that of a single drug; and oxidation resistance, inflammation resistance, fibrosis resistance and apoptosis resistance are synchronously realized. By repairing ZO-1 / Occludin, an intestinal barrier is reconstructed, intestinal toxin entering the liver is reduced, and meanwhile, liver injury and fibrosis disappearance are improved; a new strategy is provided for high-valued transformation of ginseng resources, and the safety of the plant exosome is far higher than that of a synthetic vector.

The invention provides a preparation method and application of nanoscale coenzyme Q10 water-soluble powder, and belongs to the technical field of nanoparticle powder preparation.The preparation method comprises the following steps that coenzyme Q10, natural phospholipid, auxiliaries and absolute ethyl alcohol are mixed and stirred, high-speed shearing and vacuum evaporation are conducted, and a coenzyme Q10 lipid compound is obtained; soybean soluble polysaccharide, pullulan, tara gum and deionized water are mixed, stirred and cooled, and a polysaccharide aqueous solution is obtained; adding the protein powder into deionized water, mixing and stirring, sequentially adding a polysaccharide aqueous solution, glutathione and the coenzyme Q10 lipid compound, stirring, homogenizing, performing ultrasonic treatment, filtering and drying to obtain the nano-scale coenzyme Q10 water-soluble powder. The water solubility problem of the coenzyme Q10 can be improved, and meanwhile the stability of the coenzyme Q10 is improved.

The invention provides application of adipose-derived stem cell artificial nano-vesicles in preparation of drugs for promoting platelet generation. The invention also provides an adipose-derived stem cell artificial nano-vesicle loaded with a sanguisorba monomer DMAG, and the adipose-derived stem cell artificial nano-vesicle is prepared by extracting a cell membrane of adipose-derived stem cells and entrapping DMAG by using a natural phospholipid bilayer structure of the cell membrane so as to form a nano-sized vesicle structure. The artificial nano-vesicle inherits good biocompatibility of adipose-derived stem cells, can effectively reduce immunological rejection of an organism, and protects DMAG from degradation effects such as in-vivo enzymolysis and the like; and a protective delivery system can be formed in vivo, so that rapid metabolism and elimination of DMAG in vivo are reduced, and the effective release time of DMAG in vivo is prolonged. The artificial nano-vesicles significantly increase the number of peripheral blood platelets, shorten the bleeding time and reduce the immunogenicity at the same time.