Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving ovalbumin emulsibility through composite modifying

An ovalbumin and compound modification technology, which is applied in the field of compound modification to improve the emulsification of ovalbumin, can solve the problems of difficulty in controlling reaction conditions, residual toxic components, complicated reactions, etc. Conditionally controllable effects

Active Publication Date: 2018-08-10
YANGTZE UNIVERSITY
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods mainly include phosphorylation, glycosylation, acylation and other methods, which have the characteristics of remarkable modification effect and wide application range, but there are problems such as complex reaction and easy residue of toxic components
Enzymatic modification has the advantages of strong specificity and fast speed, but it is difficult to screen suitable enzymes and control reaction conditions, and the cost is high, which limits its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving ovalbumin emulsibility through composite modifying

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of ovalbumin solution: dissolve ovalbumin in 0.01mol / L, pH 7.0 phosphate buffer solution to prepare a protein solution with a mass concentration of 0.5%, fully dissolve to obtain an ovalbumin solution;

[0031] (2) Preparation of liposomes: using the reverse evaporation method, using soybean lecithin as the wall material and cholesterol as the auxiliary wall material, the mass ratio of soybean lecithin to cholesterol is 20:1, and the preliminary liposome dispersion is prepared ;

[0032] (3) liposome particle size control: adopt micro-fluidic method to process preliminary liposome dispersion liquid, processing condition is 100MPa, circulates 8 times, obtains the liposome dispersion liquid after processing;

[0033] (4) Compounding of ovalbumin and liposomes: mix the ovalbumin solution and the treated liposome dispersion at a mass ratio of ovalbumin and soybean lecithin of 10:1, stir well until uniform, and place at 4°C for 24 hours , to obtain ovalbumin...

Embodiment 2

[0036] (1) Preparation of ovalbumin solution: dissolve ovalbumin in 0.01mol / L, pH 7.0 phosphate buffer to prepare a protein solution with a mass concentration of 1.0%, fully dissolve to obtain an ovalbumin solution;

[0037] (2) Preparation of liposomes: using the reverse evaporation method, using soybean lecithin as the wall material and cholesterol as the auxiliary wall material, the mass ratio of soybean lecithin to cholesterol is 15:1, and the preliminary liposome dispersion is prepared ;

[0038] (3) liposome particle size control: adopt micro-fluidic method to process preliminary liposome dispersion liquid, processing condition is 110MPa, circulates 6 times, obtains the liposome dispersion liquid after processing;

[0039] (4) Compounding of ovalbumin and liposomes: mix the ovalbumin solution and the treated liposome dispersion at a mass ratio of ovalbumin to soybean lecithin of 10:5, stir well until uniform, and place at 2°C for 28 hours , to obtain ovalbumin and lipos...

Embodiment 3

[0042] (1) Preparation of ovalbumin solution: dissolve ovalbumin in 0.01mol / L, pH 7.0 phosphate buffer to prepare a protein solution with a mass concentration of 1.5%, fully dissolve to obtain an ovalbumin solution;

[0043] (2) Preparation of liposomes: using the reverse evaporation method, using soybean lecithin as the wall material, and cholesterol as the auxiliary wall material, the mass ratio of soybean lecithin to cholesterol is 10:1, and the preliminary liposome dispersion is prepared ;

[0044] (3) liposome particle size control: adopt micro-fluidic method to process preliminary liposome dispersion liquid, processing condition is 125MPa, circulates 5 times, obtains the liposome dispersion liquid after processing;

[0045] (4) Compounding of ovalbumin and liposomes: mix the ovalbumin solution and the treated liposome dispersion at a mass ratio of ovalbumin and soybean lecithin of 10:10, stir well until uniform, and place at 6°C for 20 hours , to obtain ovalbumin and li...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving ovalbumin emulsibility through composite modifying. The method comprises the following steps of dissolving ovalbumin in a phosphate buffer solution to prepare a protein solution of which the mass concentration is 0.5-2%; treating a liposome dispersed solution by a micro jet method, and controlling the average particle diameter of liposome to be smaller than 100nm and compounding the ovalbumin with natural phospholipid in the mass ratio of the ovalbumin to the natural phospholipid being (10 to 1)-(10 to 20) to obtain ovalbumin and liposome so as toobtain an ovalbumin and liposome compound; and further treating the ovalbumin and liposome compound by the micro jet method, and performing freeze drying on samples to obtain the modified ovalbumin,wherein the emulsibility of the obtained ovalbumin is notably improved. Through adoption of interaction of biomacromolecule and nanoparticles, the ovalbumin and the liposome are compounded, and through assistance of the micro jet method, the emulsibility of the composite modified ovalbumin is treated. The method has the characteristics that the preparation technology is simple, and the preparationcondition is easy to control. The emulsibility of the obtained protein product is notably improved, and the application of the ovalbumin to foods is facilitated.

Description

technical field [0001] The invention belongs to the fields of food processing and food ingredients, and in particular relates to a method for compound modification to improve the emulsification of ovalbumin. Background technique [0002] my country is one of the countries with the highest output of poultry eggs in the world, but our country is still very weak in the intensive processing of poultry eggs. Improving the level of intensive processing of poultry eggs can not only effectively solve the backlog of poultry eggs and increase the added value of agricultural and sideline products, but also increase the income of farmers and promote the enthusiasm of farmers for production. [0003] Ovalbumin is an important component of egg protein. It is a typical globulin. The primary structure of the protein consists of 385 amino acids, and there are free sulfhydryl groups buried inside the hydrophobic region. Ovalbumin has good foaming and gelling properties. Due to its good foam...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/04
CPCA23J3/04
Inventor 丁保淼
Owner YANGTZE UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com