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Bay oil and nano-silver embedded liposome/chitosan antibacterial and antioxidant coating liquid as well as preparation method and application thereof

A nano-silver and liposome technology, applied in the field of food packaging materials, can solve the problems of low antibacterial activity and single function, and achieve the effect of long-lasting activity, simple process and improved thermal stability

Active Publication Date: 2019-04-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan itself has low antibacterial activity and has a single function

Method used

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  • Bay oil and nano-silver embedded liposome/chitosan antibacterial and antioxidant coating liquid as well as preparation method and application thereof
  • Bay oil and nano-silver embedded liposome/chitosan antibacterial and antioxidant coating liquid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of the liposome / chitosan antibacterial and anti-oxidation coating solution embedding laurel essential oil and nano silver, the specific steps are as follows:

[0043] (1) Under stirring conditions, add phospholipids and cholesterol to absolute ethanol to fully dissolve, the concentration of phospholipids is 1 g / L, the ratio of cholesterol to phospholipids is 1:8, and then add laurel essential oil accounting for 0.1% of the total volume Antioxidant, mix evenly, remove the solvent to form a composite film;

[0044](2) Place the obtained composite membrane in ultrapure water or phosphate buffer solution containing 0.001% lignin-coated nano-silver composite antibacterial agent for oscillating hydration (the volume ratio of the hydration solution to the phospholipid mass ratio is 100:1 ), then hydrated at 40°C for 30 minutes, and then sonicated for 1 minute with a 200 W ultrasonic cell disruptor to obtain the multifunctional liposome;

[0045] (3) Configure ...

Embodiment 2

[0047] The preparation of the liposome / chitosan antibacterial and anti-oxidation coating solution embedding laurel essential oil and nano silver, the specific steps are as follows:

[0048] (1) Under stirring conditions, add phospholipids and cholesterol to absolute ethanol to make them fully dissolved, the concentration of phospholipids is 2.5g / L, the ratio of cholesterol to phospholipids is 1:5, and then add laurel essential oil accounting for 0.25% of the total volume Antioxidant, mix evenly, remove the solvent to form a composite film;

[0049] (2) Place the obtained composite membrane in ultrapure water or phosphate buffer solution containing 0.05% lignin-coated nano-silver composite antibacterial agent for oscillating hydration (the volume ratio of the hydration solution to the phospholipid mass ratio is 400:1), Then hydrated at 50°C for 1 h, and then sonicated with a 300 W ultrasonic cell disruptor for 5 min to obtain the multifunctional liposome;

[0050] (3) Configur...

Embodiment 3

[0057] The preparation of the liposome / chitosan antibacterial and anti-oxidation coating solution embedding laurel essential oil and nano silver, the specific steps are as follows:

[0058] (1) Under stirring conditions, add phospholipids and cholesterol to absolute ethanol to fully dissolve, the concentration of phospholipids is 5 g / L, the ratio of cholesterol to phospholipids is 1:3, and then add laurel essential oil accounting for 1% of the total volume Antioxidant, mix evenly, remove the solvent to form a composite film;

[0059] (2) Place the obtained composite film in ultrapure water or phosphate buffer solution containing 1% lignin-coated nano-silver antibacterial agent for oscillating hydration (the volume ratio of the hydration solution to the phospholipid mass ratio is 600:1), Hydration at 55°C for 1.5 hours, and then ultrasonication with a 400 W ultrasonic cell disruptor for 30 minutes to obtain the multifunctional liposomes;

[0060] (3) Configure 0.8% chitosan ac...

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Abstract

The invention discloses a preparation method and application of bay oil and nano-silver embedded liposome / chitosan antibacterial and antioxidant coating liquid. The preparation method comprises the steps that a lignin-coated nano-silver antibacterial agent is prepared by adopting lignin as a reducing agent, natural phospholipid or synthetic phospholipid and cholesterol as a lipid bilayer matrix, and oil-soluble bay oil as an antioxidant enhancer are compounded with the lignin-coated nano-silver antibacterial agent so as to prepare bay oil and nano-silver embedded multifunctional liposome; andthen the multifunctional liposome and a chitosan solution are mixed so as to prepare the coating liquid. When the bay oil and nano-silver embedded liposome / chitosan antibacterial and antioxidant coating liquid is applied to food packaging, the bay oil with an antioxidant effect has a good and lasting antioxidant effect; and lignin-coated nano-silver has a good antibacterial effect and is slowly released, and therefore the toxicity of the lignin-coated nano-silver in the food application is greatly reduced, and the potential hazards to human bodies are reduced.

Description

technical field [0001] The invention relates to the field of food packaging materials, in particular to a liposome / chitosan antibacterial and anti-oxidation coating liquid embedded with laurel essential oil and nano silver, a preparation method thereof and application in food packaging. Background technique [0002] With the continuous improvement of people's living standards, fresh-cut or fresh food has developed rapidly. Traditional food packaging has a single function and cannot meet the needs of modern food storage and preservation. Therefore, it is necessary to develop a new type of multi-functional (antibacterial, anti-oxidative) food preservation material to prevent decay and rot caused by food oxidation or microbial invasion, thereby prolonging the freshness and storage period of food. Bay laurel essential oil is derived from laurel tree and is an active substance with strong antioxidant effect. Its main components are pinene or pinene, myrcene, limonene, linalool, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A23L3/3562A23L3/358
CPCA23L3/3472A23L3/3562A23L3/358A23V2002/00A23V2250/511A23V2250/21Y02A40/90
Inventor 王小英吴正国孙润仓
Owner SOUTH CHINA UNIV OF TECH
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