A micro-chamber microfluidic system for microbial growth image detection
A microfluidic system and image detection technology, applied in the field of microbial detection, can solve the problems of limiting microbial movement, microbial contact with culture medium, unfavorable microbial growth, microbial growth and movement restrictions, etc., to achieve true and reliable data, accurate measurement, The effect of less sample consumption
Active Publication Date: 2018-11-06
JINAN UNIVERSITY
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Abstract
A micro-chamber microfluidic system for microbial growth image detection is disclosed. The system includes a microscope, a constant current syringe pump, a syringe, a constant-temperature stage, a micro-chamber microfluidic chip, a collector and a CMOS video detection component. The constant-temperature stage is disposed on a microscope stage. The micro-chamber microfluidic chip is arranged on theconstant-temperature stage. A micro-chamber structure having an independent design can be used for real-time microbial culture. The CMOS video detection component is connected and fixed above the microscope, and is used for measuring morphological changes of bacteria in each micro-chamber in the micro-chamber microfluidic chip. The CMOS video detection component also includes a computation unit,and the computation unit is used for acquiring clear gray level image information of microbes based on a linear spatial filtering implementing principle and a corresponding segmentation equation and coefficients. The system effectively overcomes a technical defect that traditional microbial image detection methods are time-consuming, labor-consuming, expensive, single in function, and incapable ofacquiring long-time real-time real microbial growth image information, and has a great application prospect.
Application Domain
Bioreactor/fermenter combinationsBiological substance pretreatments +4
Technology Topic
Microfluidic chipCMOS +10
Image
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