64 results about "Crystal violet" patented technology

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation method of difunctional magnetic Fe3O4 (at) MXene (at) Ag NPs with SERS and photo-Fenton catalytic activity and application of the difunctional magnetic Fe3O4 (at) MXene (at) Ag NPs to trace detection and photo-Fenton degradation of organic pollutants in sewage. The preparation method comprises the following steps: firstly, etching an aluminum atom layer of a Ti3AlC2MAX phase by using an acidic etching solution, then carrying out a layering process to synthesize 2D MXene, and loading the 2D MXene on Fe3O4 and Ag NPs by adopting coprecipitation and electrostatic adsorption to prepare the difunctional magnetic Fe3O4 (at) MXene (at) Ag NPs composite material with SERS (Surface Enhanced Raman Scattering) and photo-Fenton catalytic activity. The detection limit of the composite material SERS substrate to crystal violet (CV) is as low as 1.08 * 10 <-12 > M, and the degradation rate of CV within 40 min reaches 91.5% under visible light irradiation and Fenton effect. The magnetic property of the composite material is also convenient to recycle and reuse, excellent SERS sensitivity can be kept even after 8 times of circulation, relatively strong photocatalytic performance can be kept even after 3 times of circulation, and the composite material is a very promising wastewater treatment material.

The invention belongs to the technical field of microorganisms, and particularly discloses high-activity lactic acid bacteria powder as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out activation culture on lactic acid bacteria in a liquid culture medium to obtain an activated bacteria solution; adjusting the concentration of the activated bacterial liquid, standing and incubating, and in the incubation process, dynamically measuring the formation amount of a biological membrane by adopting a crystal violet staining method, and determining the optimal biological membrane incubation time of the lactic acid bacteria strain; adjusting the concentration of the activated bacterial liquid, standing and incubating to the determined optimal biological membrane incubation time; washing off thalli which are not adhered to the upper layer by using the sterile PBS, and centrifugally collecting biofilm state lactic acid bacteria which are adhered to the bottom of the culture container; and re-suspending the collected biofilm state lactic acid bacteria, and drying to obtain the high-activity lactic acid bacteria powder. According to the preparation method, the drying survival rate of the strain is remarkably improved, and the resistance of lactic acid bacteria to stress such as dehydration, heat stress and cold stress in the drying process is effectively enhanced.

The invention relates to the technical field of antibacterial preparations, and discloses an application of etoposide in an antibacterial preparation and the antibacterial preparation, the antibacterial preparation is a biofilm inhibitor, and etoposide inhibits formation of a biofilm or destroys the formed biofilm by down-regulating gene expression of the bacterial biofilm, so that the antibacterial effect of etoposide is improved. The biofilm is a biofilm of streptococcus mutans ATCC700610, and the biofilm is a biofilm of streptococcus mutans The antibacterial preparation comprises an antibacterial drug, an oral care product, a medical coating and an anti-caries preparation. MIC and MBC determination tests, growth curve tests, crystal violet staining tests, bacterium living / death staining tests and qRT-PCR tests prove that etoposide can effectively down-regulate biofilm related gene expression (such as gtfB, gtfC, gtfD and the like), inhibit formation of a bacterial biofilm or destroy a formed biofilm, and achieve an antibacterial effect.

This invention discloses a molecularly switched adsorbent for adsorbing triphenylmethane dyes and its application, belonging to the field of wastewater treatment technology. The molecularly switched adsorbent proposed in this invention can rapidly remove triphenylmethane dyes such as crystal violet, malachite green, and methylene blue dyes from water; it can remove tens of ppm concentrations of crystal violet, malachite green, and methylene blue dyes from water in a very short time (90 min), with a removal rate of ≥92%. Moreover, this adsorbent can maintain a removal rate of ≥90% under a wide pH range (6-11) and high ion concentration conditions (1M). The activation and regeneration conditions of the adsorbent of this invention are mild; dye molecules can be removed by elution in a low-concentration alkaline solution only a few times. The adsorbent can be continuously recycled at least 10 times without significant attenuation of adsorption capacity.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention relates to a method for staining bacteria, comprising the steps of a) staining a bacteria sample by means of a solution of crystal violet, b) adding Lugol's solution to the stained bacteria sample, c) rinsing with distilled water, d) adding a solution of safranine-O and fuchsin in diethylene glycol monoethyl ether, and e) rinsing with distilled water, wherein the method is carried out substantially without alkanol.

The invention discloses a selective detection and monitoring culture medium for streptococcus sobrinus as well as a preparation method and application of the selective detection and monitoring culture medium, and belongs to the technical field of microorganisms. The culture medium comprises a carbon source, a nitrogen source, 4-5g / L of monopotassium phosphate, an additive and 15g / L of agar, the carbon source comprises 1-5g / L of glucose and 40-50g / L of sucrose; the nitrogen source comprises 12-18g / L of multivalent peptone and 3-7g / L of casein tryptone; the additive is prepared from 0.9-1.2 mg / L of crystal violet, 0.05-0.08 g / L of trypan blue, 2-10 mg / L of potassium tellurite, 40-60 mg / L of carumonan and 70-100 mg / L of sulfadimidine. The selective detection and monitoring culture medium for streptococcus sobrinus is high in growth selectivity, good in gram-positive bacterium growth inhibition effect, capable of well inhibiting fungus growth, sensitive in streptococcus sobrinus detection, high in specificity, simple in preparation procedure and suitable for industrial production. The practical operation feasibility is high, and the application of the culture medium for selectively detecting and monitoring the distant streptococcus is also included.

The invention relates to the field of wastewater treatment, and provides a reusable SERS (Surface Enhanced Raman Scattering) substrate of Ag / Ag3PO4 / MXene, a preparation method of the SERS substrate and application of the SERS substrate in degradation / detection of crystal violet, and the SERS substrate can be used as a surface enhanced Raman scattering substrate for efficiently detecting organic dyes and has the capability of photocatalytic degradation of organic pollutants. And through the synergistic effect of Ag3PO4 and MXene, the SERS signal of the Ag nanoparticles is obviously improved, and the detection sensitivity to the dye is enhanced. The composite material shows excellent performance in the aspect of photocatalytic degradation, the absorption of visible light is remarkably improved through the Ag nanoparticle plasmon resonance effect, separation and transmission of photo-generated charge carriers are promoted, and the photocatalytic degradation efficiency is improved. As an SERS (Surface Enhanced Raman Scattering) sensor, the detection limit on dye crystal violet is 3.82 * 10 <-11 > M, and the SERS sensor has relatively high sensitivity; the CV degradation rate of the composite material is 83.64% within 90 minutes, and the composite material has good reusability.

The invention discloses a method for detecting malachite green, crystal violet and methylene blue, which is characterized in that two different haptens are respectively used as an immunogen and a coating antigen to obtain a high-sensitivity monoclonal antibody for resisting malachite green, crystal violet and methylene blue. The invention further establishes a chemiluminescence immune method for detecting malachite green, crystal violet and methylene blue based on biotin-streptavidin, and the method has the advantages of rapidness, sensitivity, low cost and high throughput, and is suitable for simultaneously detecting the residual quantity of malachite green, crystal violet or methylene blue in aquatic products.

The invention discloses a triphenylmethane dye metabolite rapid detection method based on magnetic nano-enzyme selective oxidation and a preparation method of magnetic nano-enzyme, a novel nano-enzyme MnFe2O4 / PSS of sodium polystyrenesulfonate (PSS) modified magnetic ferrite (MnFe2O4) is prepared by adopting a coprecipitation method, the morphology and Zeta potential of the nano-enzyme are changed by doping PSS, and the magnetic nano-enzyme can be used for rapidly detecting the metabolite of the triphenylmethane dye. The nano-enzyme can react with leuco crystal violet and leuco malachite green more quickly, the leuco crystal violet and the leuco malachite green are oxidized into corresponding crystal violet and malachite green without further oxidation, after the reaction is completed, the error of a detection system can be reduced by utilizing magnetic separation, and by detecting the ultraviolet absorption peak intensity of oxidation products of the nano-enzyme, the detection accuracy is improved. A triphenylmethane dye metabolite oxidation-detection system is established, the magnetic nano-enzyme has good magnetism, the influence of the nano-enzyme on the absorbance of the system can be reduced through simple magnetic separation, and the purposes of reducing experimental errors and accurately detecting are achieved.

The application discloses a kind of synthesis method for preparing structure is hydrangea-like MnCdS / In2S3 / NiAl-LDH photocatalyst by two-step hydrothermal method, belong to composite material and photocatalytic technical field.Sulfaceticamide (TAA), cadmium acetate dihydrate ((CH3COO)2Cd·2H2O), manganese acetate tetrahydrate (Mn(CH3COO)2·4H2O), indium nitrate 4.5 hydrate (In(NO3)3·4.5H2O), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), aluminum nitrate nonahydrate (Al(NO3)3·9H2O), ammonium fluoride (NH4F), urea (CO(NH2)2) are used as raw materials, monomer is treated by two-step hydrothermal method, and hydrangea-like MnCdS / In2S3 / NiAl-LDH composite photocatalyst is obtained.The surface morphology, microstructure and photocatalytic activity are determined, and the product performance is greatly improved in the photocatalysis of degrading organic pollutants crystal violet and photolysis water to produce hydrogen.

The invention discloses a mechanochromic molecule based on crystal violet lactam, a bulk polymer and application of the mechanochromic molecule and the bulk polymer, a force sensitive unit and an introduction process of a polymerizable functional group are organically combined, the synthesis steps are effectively simplified, the preparation cost is reduced, and a new thought is provided for design of a controllable and specific mechanochromic material. According to the invention, accurate regulation and control of color and force-induced discoloration behaviors are realized through molecular structure optimization and polymer structure design. The molecule has the advantages of simple and convenient synthesis steps, cheap raw materials and low cost, can realize large-scale preparation, and shows a stable and remarkable force-induced discoloration effect in a polymer material during mechanical compression or stretching, so that the molecule has a wide application prospect.

The invention discloses a humidity-driven deformation color-changing flexible film based on a magnetic field induction method and a preparation method and application thereof, and relates to the technical field of humidity-driven flexible films. The preparation method comprises the following steps: (1) preparing magnetic MXene coated Fe3O4 particles; (2) preparing a Nafion / crystal violet / MXene coated Fe3O4 mixed solution; (3) inducing the accumulation of the magnetic MXene coated Fe3O4 particles in the mixed solution through a magnetic field; and (4) preparing the structured Nafion-crystal violet-MXene coated Fe3O4 film with the corresponding humidity. The obtained film is made of Nafion / crystal violet / MXene coated Fe3O4 materials, when the film is stimulated by humidity, the film can be bent and deformed, the color is changed from yellow to green, and the film has the advantages of being high in deformation capacity and obvious in color changing effect and has application potential in the fields of drivers, bionic robots and the like.

The application discloses a europium functionalized metal-organic framework double-emission fluorescent probe and a preparation method and application thereof, relates to the field of organic metal frameworks and fluorescent detection, and the preparation method comprises the following steps: mixing ZrCl4, pyromellitic acid, 6-hydroxy-2-naphthoic acid and water, adding acetic acid to obtain a mixture; the mixture is heated to reflux, centrifuged to obtain a residue, washed and dried to obtain UCHNA; the UCHNA is dispersed in an aqueous solution of Eu(NO3)3.6H2O, heated to react, the white precipitate is collected, washed and dried, and the Eu-MOF is obtained. 3+ The application has the beneficial effect that a luminescent ligand 6-hydroxy-2-naphthoic acid is used to modify a UiO-66 type MOF, and Eu 3+ -MOF is synthesized through post-modification and reasonable design, and the Eu-MOF can selectively and rapidly respond to crystal violet (CV) and quinine (QN) through different response modes, and has a low detection limit, which is superior to most reported sensors.

The present invention relates to the technical field of fluorescent probes, and in particular to a fluorescent probe, test paper, and preparation method and application thereof for detecting malachite green, a triphenylmethane compound. The fluorescent probe comprises a fluorescent ion pair formed by rhodamine B (RB) and perfluorinated tetraphenylborate ion (F5TPB); the triphenylmethane compounds are malachite green (MG), crystal violet (CV), and methyl violet (MV); the detection limit for malachite green is 0.1776 μmol / L (3σ / k, n=11); the present invention also provides a test paper for detecting triphenylmethane compounds, which can simply and conveniently detect malachite green and its residual amount.

The application belongs to the technical field of disease detection, and particularly relates to application of triphenylmethane compounds in detection of Alzheimer's disease. The application is accidentally discovered that triphenylmethane compounds, especially crystal violet, can be inserted into misfolded beta-sheets of A beta oligomers, so as to cause change of spectrum. Macroscopically, oxidation and discoloration are caused, so that the triphenylmethane compounds can be used for monitoring and early screening of Alzheimer's disease patients. Compared with antigen-antibody preparation of antigen-antibody immunoreaction products, the product in the application has the defects of complicated antigen-antibody preparation and high cost, and the product in the application has low cost. Meanwhile, the main component of the application is a chemical reagent, has good stability, does not need refrigeration storage condition, is convenient to transport, and is convenient to use. Meanwhile, the technical scheme of the application effectively avoids the cleaning process of enzyme-labeled plates and chemiluminescence experiments, and does not need special equipment, so that the basic medical institutions can use the application, and therefore the application has good practical application value and application prospect.

The invention relates to the technical field of escherichia coli biofilm formation determination, in particular to a method for determining the escherichia coli biofilm formation capacity through a crystal violet staining method. The method comprises the steps of bacterial liquid preparation, microwell plate sterilization, inoculation and culture, washing and fixation, dyeing and dissolution and quantitative detection. The method comprises the following steps: systematically analyzing biological characteristics such as biofilm forming ability, drug resistance, drug resistance genes and virulence genes of avian pathogenic escherichia coli through a multi-dimensional evaluation system, introducing a local isolated strain in Gansu province as a contrast, screening a strong biofilm forming strain in combination with ANOVA, and detecting the virulence genes through PCR (Polymerase Chain Reaction). The invention finds that the drug resistance rate of beta-lactams in a strong biofilm strain is as high as 82.3%, target data is provided for regional antibiotic control, meanwhile, a biofilm strength-virulence gene-drug resistance spectrum correlation model is established, the limitation of single phenotypic research is broken through, and the clinical treatment guidance value is improved.

The invention discloses a composite hydrogel adsorbent as well as a preparation method and application thereof, and relates to the technical field of water pollution treatment. The composite hydrogel adsorbent comprises the following raw materials: chitosan, acrylic acid, sodium methallyl sulfonate and coffee grounds. Wherein the molar weight of the sodium methallyl sulfonate is 2.3-2.9% of the molar weight of the acrylic acid; the mass of the chitosan is 0.09-6.3% of the total mass of the sodium methallyl sulfonate and the acrylic acid; the mass of the coffee grounds is 0.4-1.8% of the total mass of the sodium methallyl sulfonate and the acrylic acid, and the composite hydrogel adsorbent is prepared through a free radical polymerization reaction. The composite hydrogel adsorbent disclosed by the invention is high in adsorption capacity and high in speed on dyes, especially methylene blue and crystal violet, in sewage, the sewage treatment efficiency is remarkably improved, an efficient, economical and environment-friendly solution is provided for dye wastewater treatment, and the composite hydrogel adsorbent has a good practical application prospect.

The invention discloses a laccase mutant and application thereof, the mutant is obtained by mutating wild laccase BstCotA from bacillus subtilis, the amino acid sequence of the mutant is as shown in SEQ ID NO.1, and the mutant is mutated into at least one of E186A, N65G, D185G and N311G. The invention further discloses a preparation method of the laccase mutant. Compared with a wild type, the mutant has the advantages that the catalytic activity is obviously improved, and the relative enzyme activity is higher under the conditions that the temperature is 37 DEG C and the pH value is 5.0; the pH stability and the thermal stability are enhanced, and the activity in the range of 40-90 DEG C exceeds 75%; the dye decolorization efficiency is higher, the decolorization rate of amino black 10B reaches 28%-35%, the decolorization rate of crystal violet reaches 35%-45%, and complex printing and dyeing wastewater can be efficiently treated; the lignin degradation capacity is better, the degradation rates within 1 hour and 2 hours are higher than those of the wild type, and pulping bleaching and biomass resource utilization are facilitated.

The invention relates to the field of drug sensitivity diagnosis, in particular to a swine-derived broad-spectrum enrichment medium as well as a preparation method and application thereof. The pig-derived broad-spectrum enrichment culture medium is prepared from the following raw materials in parts by weight: 5.0 to 10.0 parts of peptone, 3.0 to 8.0 parts of yeast extract powder, 5.0 to 8.0 parts of sodium chloride, 5.0 to 10.0 parts of lactose, 0.5 to 2.5 parts of cholate, 0.002 to 0.005 part of crystal violet, 0.002 to 0.010 part of neutral red, 15.0 to 20.0 parts of agar, 1.0 to 3.5 parts of glucose, 1.0 to 3.5 parts of dipotassium phosphate, 2.5 to 5.0 parts of disodium hydrogen phosphate, 0.2 to 0.5 part of sodium thiosulfate and 1000 parts of distilled water. 50-100 parts of filtered and sterilized newborn calf serum and 1.0-2.5 parts of NAD (Nicotinamide Adenine Dinucleotide) with the mass fraction of 1%. According to the invention, the formula of the enrichment culture medium is optimized, the liquid culture medium is used as the enrichment culture medium for the drug sensitive test, and a new acid-base indicator is selected according to the biochemical characteristics of bacteria, so that the process of the drug sensitive test is simplified.

This invention discloses an Ag-Bi 24 Br 10 O 31 This paper discusses the preparation and application of Bi₂O₂S heterojunction composite materials, belonging to the field of SERS detection technology. First, Bi₂O₂S heterojunction composite materials are prepared using a hydrothermal method. 24 Br 10 O 31 Nanosheets, then with Bi 24 Br 10 O 31 Bi2O2S was constructed by secondary hydrothermal in-situ growth of Bi2O2S on nanosheets as a substrate. 24 Br 10 O 31 A Bi₂O₂S heterostructure was formed, followed by chemical reduction to load Ag nanoparticles, and then dried to obtain the target composite SERS substrate. This material utilizes the localized surface plasmon effect of Ag to form a strong SERS "hot spot," exhibiting excellent Raman enhancement performance for crystal violet, enabling highly sensitive detection of crystal violet. This method is simple, uses readily available raw materials, and produces a material that combines excellent SERS enhancement performance with photocatalytic activity, showing broad application prospects in environmental monitoring and pollution control.

The invention discloses a molecular imprinting colorimetric sensor for detecting hepatitis B virus as well as a preparation method and application of the molecular imprinting colorimetric sensor. The sensor takes an aminated zeolite imidazate framework material (ZIF-8-NH2) with peroxidase-like activity as a catalytic core, after surface double-bond functionalization, a molecularly imprinted polymer (MIPs) layer is constructed on the surface through a free radical polymerization reaction by taking the aminated zeolite imidazate framework material as a carrier and taking HBV as a template molecule. According to the sensor, after HBV is specifically recognized and captured through MIPs, the catalytic efficiency of ZIF-8 on hydrogen peroxide is changed, then the generation amount of hydroxyl radicals is regulated and controlled, and finally visual and quantitative detection of HBV is achieved through the color fading reaction of the hydroxyl radicals and chromogenic dye crystal violet. The sensor prepared by the invention has the advantages of high sensitivity, strong specificity, low cost, simplicity and convenience in operation, rapidness in detection and the like, and can be used for rapid screening and on-site instant detection of hepatitis B viruses.

The invention relates to the technical field of bacterial detection, in particular to a Salmonella pullorum high-throughput detection method which comprises the following steps: inoculating Salmonella pullorum to a TSB culture medium, culturing to obtain a bacterial liquid, inactivating the bacterial liquid, centrifuging to remove supernatant, dyeing by using a crystal violet ethanol solution, adjusting the concentration of the Salmonella pullorum to 5 * 10 < 7 > CFU / mL to 5 * 10 < 9 > CFU / mL, and carrying out high-throughput detection on the Salmonella pullorum. And preparing to obtain a salmonella gallinarum antigen solution. The method comprises the following steps: diluting serum to be detected, adding into a porous plate, drying and fixing, sealing by using a PBS (Phosphate Buffer Solution), adding a salmonella gallinarum antigen solution, incubating, washing, and observing by naked eyes or measuring by using a microplate reader so as to judge the result. According to the invention, high-flux, low-cost and rapid detection of pullorum disease can be realized, the problem of insufficient accuracy of traditional manual interpretation is solved, and a technical method is provided for identification and diagnosis of pullorum disease.

The invention relates to the technical field of microorganisms, and discloses a VBNC Escherichia coli induction method, a VBNC Escherichia coli resuscitation medium and application, and the VBNC Escherichia coli resuscitation medium comprises Rpf recombinant protein, MgSO4, sodium pyruvate, NAD, peptone, yeast extract, lactose, sodium chloride, cholate, neutral red, crystal violet and agar. The VBNC escherichia coli resuscitation culture medium provided by the invention can be used for successfully culturing escherichia coli in a VBNC state.

The invention discloses a quaternary composite photocatalyst based on three-dimensional ordered macroporous titanium dioxide as well as a preparation method and application of the quaternary composite photocatalyst. The quaternary composite photocatalyst comprises 3DOM TiO2 and elementary substances Ag, Ag2S and ZnSe which are uniformly loaded on the surface of the 3DOM TiO2 and in pore channels. The composite material shows remarkably enhanced photocatalytic activity in the aspects of organic pollutant crystal violet degradation and water photolysis hydrogen production; the antibacterial effect on escherichia coli is excellent.

The application discloses a kind of composite hydrogel adsorbent and its preparation method and application, it is related to water pollution treatment technical field.The raw material of the composite hydrogel adsorbent of the present application includes chitosan, acrylic acid, sodium methacryl sulfonate and coffee residue;Among them, the molar mass of sodium methacryl sulfonate is 2.3~2.9% of the molar mass of acrylic acid;The mass of chitosan is 0.09~6.3% of the total mass of sodium methacryl sulfonate and acrylic acid;The mass of coffee residue is 0.4~1.8% of the total mass of sodium methacryl sulfonate and acrylic acid, and the composite hydrogel adsorbent is prepared by free radical polymerization reaction.The composite hydrogel adsorbent of the present application has strong adsorption capacity and fast rate for dyes in wastewater, especially methylene blue and crystal violet, significantly improves the efficiency of wastewater treatment, provides an efficient, economical and environmentally friendly solution for dye wastewater treatment, and has good practical application prospect.

The invention discloses a visible / ultraviolet dual-light synergistically enhanced substrate, a preparation method thereof and application of the visible / ultraviolet dual-light synergistically enhanced substrate in rapid detection of veterinary drugs. According to the method, a gas-liquid interface self-assembly technology is combined with a light welding process, and the heterojunction composite Raman substrate Bi: ZnO-SUSNs of a Bi-doped zinc oxide nano array and urchin-shaped silver nanoparticles is prepared. The Bi: ZnO-SUSNs composite Raman substrate disclosed by the invention has excellent visible light / ultraviolet light dual response characteristics, and the detection sensitivity to target molecules can be greatly improved under dual-light driving by utilizing a PIERS mechanism. According to the method, trace detection of veterinary drugs such as malachite green, crystal violet and furacilin can be realized without complex pretreatment. In addition, the composite substrate also has a photocatalytic degradation function, can realize self-cleaning and reutilization, and has the advantages of low preparation cost, high detection speed, good repeatability and the like.

This invention provides a silver nanoparticle-modified PS sphere-silver sphere cavity composite structure SERS substrate, its preparation method, and its application, belonging to the field of Raman spectroscopy detection technology. The method uses a polystyrene (PS) microsphere array as a template, combining tilted rotational evaporation and physical flipping processes to construct a 3D silver nanoparticle array structure. Under unwashed conditions, silver layers of different thicknesses (10~80 nm) are vertically evaporated onto the exposed surface of the PS microspheres, allowing silver nanoparticles to grow directly on the PS microsphere surface, forming a multi-level composite structure of "silver nanoparticle-silver sphere cavity". The substrate with a vertically evaporated 20 nm silver layer exhibits the best SERS enhancement performance, with a detection limit as low as 10 for crystal violet (CV). ‑11 M, the relative standard deviation (RSD) of 50 random test points is 7.42%.

The invention provides a preparation method for reducing the angle of a photoresist inverted trapezoidal isolation column, which comprises the following steps: preparing a crystal violet solution with the concentration of 50-200mg / L in a yellow light environment; the photoresist added with the crystal violet solution is obtained; the crystal violet solution and the polysorbate-20 solution are added into the photoresist; placing the photoresist added with the crystal violet solution and the polysorbate-20 solution in a refrigerator at 1-10 DEG C for 20-30 hours, and screening out the photoresist without precipitation as the final photoresist; the final inverted trapezoidal isolation column is prepared; according to the method, the chamfer angle of the exposed pattern can be effectively increased, so that the side wall is changed into a more inclined slope shape from a steep shape.