31 results about "Crystal violet" patented technology

The invention belongs to the technical field of microorganisms, and particularly discloses high-activity lactic acid bacteria powder as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out activation culture on lactic acid bacteria in a liquid culture medium to obtain an activated bacteria solution; adjusting the concentration of the activated bacterial liquid, standing and incubating, and in the incubation process, dynamically measuring the formation amount of a biological membrane by adopting a crystal violet staining method, and determining the optimal biological membrane incubation time of the lactic acid bacteria strain; adjusting the concentration of the activated bacterial liquid, standing and incubating to the determined optimal biological membrane incubation time; washing off thalli which are not adhered to the upper layer by using the sterile PBS, and centrifugally collecting biofilm state lactic acid bacteria which are adhered to the bottom of the culture container; and re-suspending the collected biofilm state lactic acid bacteria, and drying to obtain the high-activity lactic acid bacteria powder. According to the preparation method, the drying survival rate of the strain is remarkably improved, and the resistance of lactic acid bacteria to stress such as dehydration, heat stress and cold stress in the drying process is effectively enhanced.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The invention discloses a triphenylmethane dye metabolite rapid detection method based on magnetic nano-enzyme selective oxidation and a preparation method of magnetic nano-enzyme, a novel nano-enzyme MnFe2O4 / PSS of sodium polystyrenesulfonate (PSS) modified magnetic ferrite (MnFe2O4) is prepared by adopting a coprecipitation method, the morphology and Zeta potential of the nano-enzyme are changed by doping PSS, and the magnetic nano-enzyme can be used for rapidly detecting the metabolite of the triphenylmethane dye. The nano-enzyme can react with leuco crystal violet and leuco malachite green more quickly, the leuco crystal violet and the leuco malachite green are oxidized into corresponding crystal violet and malachite green without further oxidation, after the reaction is completed, the error of a detection system can be reduced by utilizing magnetic separation, and by detecting the ultraviolet absorption peak intensity of oxidation products of the nano-enzyme, the detection accuracy is improved. A triphenylmethane dye metabolite oxidation-detection system is established, the magnetic nano-enzyme has good magnetism, the influence of the nano-enzyme on the absorbance of the system can be reduced through simple magnetic separation, and the purposes of reducing experimental errors and accurately detecting are achieved.

The application discloses a kind of synthesis method for preparing structure is hydrangea-like MnCdS / In2S3 / NiAl-LDH photocatalyst by two-step hydrothermal method, belong to composite material and photocatalytic technical field.Sulfaceticamide (TAA), cadmium acetate dihydrate ((CH3COO)2Cd·2H2O), manganese acetate tetrahydrate (Mn(CH3COO)2·4H2O), indium nitrate 4.5 hydrate (In(NO3)3·4.5H2O), nickel nitrate hexahydrate (Ni(NO3)2·6H2O), aluminum nitrate nonahydrate (Al(NO3)3·9H2O), ammonium fluoride (NH4F), urea (CO(NH2)2) are used as raw materials, monomer is treated by two-step hydrothermal method, and hydrangea-like MnCdS / In2S3 / NiAl-LDH composite photocatalyst is obtained.The surface morphology, microstructure and photocatalytic activity are determined, and the product performance is greatly improved in the photocatalysis of degrading organic pollutants crystal violet and photolysis water to produce hydrogen.

The invention discloses a mechanochromic molecule based on crystal violet lactam, a bulk polymer and application of the mechanochromic molecule and the bulk polymer, a force sensitive unit and an introduction process of a polymerizable functional group are organically combined, the synthesis steps are effectively simplified, the preparation cost is reduced, and a new thought is provided for design of a controllable and specific mechanochromic material. According to the invention, accurate regulation and control of color and force-induced discoloration behaviors are realized through molecular structure optimization and polymer structure design. The molecule has the advantages of simple and convenient synthesis steps, cheap raw materials and low cost, can realize large-scale preparation, and shows a stable and remarkable force-induced discoloration effect in a polymer material during mechanical compression or stretching, so that the molecule has a wide application prospect.

The invention discloses a humidity-driven deformation color-changing flexible film based on a magnetic field induction method and a preparation method and application thereof, and relates to the technical field of humidity-driven flexible films. The preparation method comprises the following steps: (1) preparing magnetic MXene coated Fe3O4 particles; (2) preparing a Nafion / crystal violet / MXene coated Fe3O4 mixed solution; (3) inducing the accumulation of the magnetic MXene coated Fe3O4 particles in the mixed solution through a magnetic field; and (4) preparing the structured Nafion-crystal violet-MXene coated Fe3O4 film with the corresponding humidity. The obtained film is made of Nafion / crystal violet / MXene coated Fe3O4 materials, when the film is stimulated by humidity, the film can be bent and deformed, the color is changed from yellow to green, and the film has the advantages of being high in deformation capacity and obvious in color changing effect and has application potential in the fields of drivers, bionic robots and the like.

The application discloses a europium functionalized metal-organic framework double-emission fluorescent probe and a preparation method and application thereof, relates to the field of organic metal frameworks and fluorescent detection, and the preparation method comprises the following steps: mixing ZrCl4, pyromellitic acid, 6-hydroxy-2-naphthoic acid and water, adding acetic acid to obtain a mixture; the mixture is heated to reflux, centrifuged to obtain a residue, washed and dried to obtain UCHNA; the UCHNA is dispersed in an aqueous solution of Eu(NO3)3.6H2O, heated to react, the white precipitate is collected, washed and dried, and the Eu-MOF is obtained. 3+ The application has the beneficial effect that a luminescent ligand 6-hydroxy-2-naphthoic acid is used to modify a UiO-66 type MOF, and Eu 3+ -MOF is synthesized through post-modification and reasonable design, and the Eu-MOF can selectively and rapidly respond to crystal violet (CV) and quinine (QN) through different response modes, and has a low detection limit, which is superior to most reported sensors.

The application belongs to the technical field of disease detection, and particularly relates to application of triphenylmethane compounds in detection of Alzheimer's disease. The application is accidentally discovered that triphenylmethane compounds, especially crystal violet, can be inserted into misfolded beta-sheets of A beta oligomers, so as to cause change of spectrum. Macroscopically, oxidation and discoloration are caused, so that the triphenylmethane compounds can be used for monitoring and early screening of Alzheimer's disease patients. Compared with antigen-antibody preparation of antigen-antibody immunoreaction products, the product in the application has the defects of complicated antigen-antibody preparation and high cost, and the product in the application has low cost. Meanwhile, the main component of the application is a chemical reagent, has good stability, does not need refrigeration storage condition, is convenient to transport, and is convenient to use. Meanwhile, the technical scheme of the application effectively avoids the cleaning process of enzyme-labeled plates and chemiluminescence experiments, and does not need special equipment, so that the basic medical institutions can use the application, and therefore the application has good practical application value and application prospect.

The invention discloses a laccase mutant and application thereof, the mutant is obtained by mutating wild laccase BstCotA from bacillus subtilis, the amino acid sequence of the mutant is as shown in SEQ ID NO.1, and the mutant is mutated into at least one of E186A, N65G, D185G and N311G. The invention further discloses a preparation method of the laccase mutant. Compared with a wild type, the mutant has the advantages that the catalytic activity is obviously improved, and the relative enzyme activity is higher under the conditions that the temperature is 37 DEG C and the pH value is 5.0; the pH stability and the thermal stability are enhanced, and the activity in the range of 40-90 DEG C exceeds 75%; the dye decolorization efficiency is higher, the decolorization rate of amino black 10B reaches 28%-35%, the decolorization rate of crystal violet reaches 35%-45%, and complex printing and dyeing wastewater can be efficiently treated; the lignin degradation capacity is better, the degradation rates within 1 hour and 2 hours are higher than those of the wild type, and pulping bleaching and biomass resource utilization are facilitated.

This invention discloses an Ag-Bi 24 Br 10 O 31 This paper discusses the preparation and application of Bi₂O₂S heterojunction composite materials, belonging to the field of SERS detection technology. First, Bi₂O₂S heterojunction composite materials are prepared using a hydrothermal method. 24 Br 10 O 31 Nanosheets, then with Bi 24 Br 10 O 31 Bi2O2S was constructed by secondary hydrothermal in-situ growth of Bi2O2S on nanosheets as a substrate. 24 Br 10 O 31 A Bi₂O₂S heterostructure was formed, followed by chemical reduction to load Ag nanoparticles, and then dried to obtain the target composite SERS substrate. This material utilizes the localized surface plasmon effect of Ag to form a strong SERS "hot spot," exhibiting excellent Raman enhancement performance for crystal violet, enabling highly sensitive detection of crystal violet. This method is simple, uses readily available raw materials, and produces a material that combines excellent SERS enhancement performance with photocatalytic activity, showing broad application prospects in environmental monitoring and pollution control.

The invention discloses a molecular imprinting colorimetric sensor for detecting hepatitis B virus as well as a preparation method and application of the molecular imprinting colorimetric sensor. The sensor takes an aminated zeolite imidazate framework material (ZIF-8-NH2) with peroxidase-like activity as a catalytic core, after surface double-bond functionalization, a molecularly imprinted polymer (MIPs) layer is constructed on the surface through a free radical polymerization reaction by taking the aminated zeolite imidazate framework material as a carrier and taking HBV as a template molecule. According to the sensor, after HBV is specifically recognized and captured through MIPs, the catalytic efficiency of ZIF-8 on hydrogen peroxide is changed, then the generation amount of hydroxyl radicals is regulated and controlled, and finally visual and quantitative detection of HBV is achieved through the color fading reaction of the hydroxyl radicals and chromogenic dye crystal violet. The sensor prepared by the invention has the advantages of high sensitivity, strong specificity, low cost, simplicity and convenience in operation, rapidness in detection and the like, and can be used for rapid screening and on-site instant detection of hepatitis B viruses.

The application discloses a kind of composite hydrogel adsorbent and its preparation method and application, it is related to water pollution treatment technical field.The raw material of the composite hydrogel adsorbent of the present application includes chitosan, acrylic acid, sodium methacryl sulfonate and coffee residue;Among them, the molar mass of sodium methacryl sulfonate is 2.3~2.9% of the molar mass of acrylic acid;The mass of chitosan is 0.09~6.3% of the total mass of sodium methacryl sulfonate and acrylic acid;The mass of coffee residue is 0.4~1.8% of the total mass of sodium methacryl sulfonate and acrylic acid, and the composite hydrogel adsorbent is prepared by free radical polymerization reaction.The composite hydrogel adsorbent of the present application has strong adsorption capacity and fast rate for dyes in wastewater, especially methylene blue and crystal violet, significantly improves the efficiency of wastewater treatment, provides an efficient, economical and environmentally friendly solution for dye wastewater treatment, and has good practical application prospect.

The invention discloses a visible / ultraviolet dual-light synergistically enhanced substrate, a preparation method thereof and application of the visible / ultraviolet dual-light synergistically enhanced substrate in rapid detection of veterinary drugs. According to the method, a gas-liquid interface self-assembly technology is combined with a light welding process, and the heterojunction composite Raman substrate Bi: ZnO-SUSNs of a Bi-doped zinc oxide nano array and urchin-shaped silver nanoparticles is prepared. The Bi: ZnO-SUSNs composite Raman substrate disclosed by the invention has excellent visible light / ultraviolet light dual response characteristics, and the detection sensitivity to target molecules can be greatly improved under dual-light driving by utilizing a PIERS mechanism. According to the method, trace detection of veterinary drugs such as malachite green, crystal violet and furacilin can be realized without complex pretreatment. In addition, the composite substrate also has a photocatalytic degradation function, can realize self-cleaning and reutilization, and has the advantages of low preparation cost, high detection speed, good repeatability and the like.

This invention provides a silver nanoparticle-modified PS sphere-silver sphere cavity composite structure SERS substrate, its preparation method, and its application, belonging to the field of Raman spectroscopy detection technology. The method uses a polystyrene (PS) microsphere array as a template, combining tilted rotational evaporation and physical flipping processes to construct a 3D silver nanoparticle array structure. Under unwashed conditions, silver layers of different thicknesses (10~80 nm) are vertically evaporated onto the exposed surface of the PS microspheres, allowing silver nanoparticles to grow directly on the PS microsphere surface, forming a multi-level composite structure of "silver nanoparticle-silver sphere cavity". The substrate with a vertically evaporated 20 nm silver layer exhibits the best SERS enhancement performance, with a detection limit as low as 10 for crystal violet (CV). ‑11 M, the relative standard deviation (RSD) of 50 random test points is 7.42%.

The invention provides a preparation method for reducing the angle of a photoresist inverted trapezoidal isolation column, which comprises the following steps: preparing a crystal violet solution with the concentration of 50-200mg / L in a yellow light environment; the photoresist added with the crystal violet solution is obtained; the crystal violet solution and the polysorbate-20 solution are added into the photoresist; placing the photoresist added with the crystal violet solution and the polysorbate-20 solution in a refrigerator at 1-10 DEG C for 20-30 hours, and screening out the photoresist without precipitation as the final photoresist; the final inverted trapezoidal isolation column is prepared; according to the method, the chamfer angle of the exposed pattern can be effectively increased, so that the side wall is changed into a more inclined slope shape from a steep shape.

The application discloses a bacterial biofilm detection probe, a detection method and application, relates to the technical field of microorganism detection and fluorescent probe application, and the fluorescent probe is of formula Ia structure. The bacterial biofilm detection probe detection method comprises the following steps: bacterial culture to form a biofilm, PBS removal of planktonic bacteria, addition of the probe incubation for 5 min, and detection of 520 nm fluorescence intensity by using a fluorescence enzyme label instrument. The application applies the fluorescent probe to biofilm detection, compared with traditional classic biofilm detection methods such as crystal violet, has the advantages of no need of cleaning, short detection period, simple operation, high accuracy, strong linear correlation, adaptation to gram-positive / negative bacteria and fungal biofilm, and provides an effective tool for detection of bacterial biofilm. The application can be used for high-throughput screening of active drugs with inhibition of biofilm formation or destruction of bacterial biofilm structure, and solves the technical problems of complicated steps, long time consumption and poor reproducibility in the prior art.

The application provides a method for removing mixed dyes in wastewater, and relates to the technical field of water treatment. The method for removing mixed dyes in wastewater provided by the application uses hexadecyl trimethyl ammonium bromide modified iron-based MOF material as a floating agent, and adopts a froth flotation method to remove rhodamine B and crystal violet in wastewater; a bubbling device is used to generate a large number of bubbles on the liquid surface, so that the floating agent attached to the target dyes floats with the bubbles, and finally, the surface foam is removed through froth flotation, so as to reduce the concentrations of crystal violet and rhodamine B dyes in the wastewater. The method has the advantages of simple equipment, simple operation, good treatment effect, low operation cost, and lays a good foundation for the removal and recovery of dyes in wastewater, and is conducive to large-scale popularization and application.

This invention provides laccase mutants and their applications. The laccase mutants, relative to the amino acid sequence of the wild-type laccase shown in SEQ ID NO:1, have one or more of the following mutations: M40K, D50N, F68L, N83T, D206A, I226V, and V268T. The laccase mutants of this invention exhibit significantly enhanced enzyme activity at 0–45°C, with peak activities at 0°C, 15°C, 30°C, and 45°C, respectively, being 3.88 times, 5.24 times, 6.47 times, and 6.42 times that of the wild-type. They also exhibit significantly enhanced enzyme activity at pH 3.0 and pH 4.0, with peak activities at pH 3.0 and pH 4.0, respectively, being 7.02 times and 6.97 times that of the wild-type. In exemplary industrial applications for the degradation of crystal violet and lignin, they have demonstrated excellent degradation capabilities, laying a solid foundation for the application of laccase.

The invention discloses a flexible SERS (Surface Enhanced Raman Scattering) chip, a preparation method of the flexible SERS chip and application of the flexible SERS chip in detection of marine food pollutants, and belongs to the technical field of analytical testing. The preparation method comprises the following steps: preparing carbon dot-silver nanoparticle composite sol through one-pot in-situ reduction; pretreating a filter paper substrate; loading the composite sol on the surface of filter paper, and drying and curing. The capillary enrichment effect of the filter paper, the electromagnetic enhancement effect of the silver nanoparticles and the charge transfer chemical enhancement effect of the carbon dot-silver nanoparticle heterogeneous interface are utilized to construct an enrichment-electromagnetic enhancement-chemical enhancement triple synergistic mechanism, and the SERS detection sensitivity, the signal uniformity and the substrate stability are remarkably improved. The preparation method is simple and low in cost, the detection limit of the prepared chip to rhodamine 6G is as low as 10-15 M, the enhancement factor is as high as 1013, in-situ'wiping-detection 'of crystal violet pollutants on the surface of a prawn body can be realized, and the chip has a wide application prospect in the field of rapid screening of a marine food safety site.

The application discloses a method for ultrafast degradation of organic dye pollutants through a microdroplet system, and belongs to the technical field of degradation of organic dye pollutants. The method comprises the following steps: forming microdroplets by spraying a solution containing organic dye pollutants to be treated through a spraying device, and making the microdroplets contact with a solid substrate to realize degradation of the organic dye pollutants; the solid substrate is a metal substrate; the organic dye pollutants include at least one of methyl blue, chrome black T, crystal violet and rhodamine B. The method has wide applicability to various toxic dyes (including methyl blue, chrome black T, crystal violet and rhodamine B), and can achieve a degradation rate of more than 90% under a circulating spraying operation. The achievements provide basic scientific support for water purification, and open up a new path for sustainable environmental remediation technology.

The invention discloses laccase and application thereof, and belongs to the field of bioengineering. The high-activity laccase Lac-11 is obtained through database mining, and compared with existing high-activity laccase BstCotA, the high-activity laccase Lac-11 has the following beneficial effects that 1, the enzyme activity is remarkably improved, the enzyme activity is improved by nearly 50% under the conditions that the temperature is 37 DEG C and the pH is 5.0, and the industrial enzyme cost is reduced; 2, the pH stability is higher, the enzyme activity retention rate is higher in the pH range of 5.0-8.0, and the method is suitable for wider acid-base environments. And 3, the thermal stability is better, the stability at the medium-high temperature interval of 50-80 DEG C is obviously higher than that of a control enzyme, and the method is suitable for an industrial high-temperature process. 4, the dye decolorization efficiency is higher, the decolorization rates of amino black 10B and crystal violet respectively reach 31% and 37%, and printing and dyeing wastewater can be efficiently treated. And 5, the lignin degradation capacity is outstanding, the degradation rates within 1 hour, 2 hours and 24 hours are 22%, 25% and 58% respectively, and pulping bleaching and biomass recycling are facilitated.

The invention relates to the technical field of enzyme engineering, and provides an engineering bacterium for producing fungal laccase as well as a construction method and application of the engineering bacterium, and the engineering bacterium is a pichia pastoris engineering bacterium into which a fusarium fujikuroi laccase gene Lac-1 is introduced. The construction method comprises the following steps: cloning the laccase gene, and connecting the laccase gene to an expression vector pPICZ alpha A to obtain a recombinant vector pPICZ alpha A-Lac; and after being linearized, the recombinant vector is electrically transformed into pichia pastoris GS115 for heterologous expression. The invention also provides a separation and purification method of the laccase produced by the engineering bacterium, which comprises the steps of ammonium sulfate precipitation, ion exchange chromatography and gel filtration, and immobilization of the purified recombinant laccase by a covalent method. The method has the advantages that under the acidic condition, under the condition that ABTS (2, 2 '-nitrogen-bis (3-ethylbenzothiazole-6-sulfonic acid)) is used as a mediator, the efficient decolorization capacity is shown for various dyes (such as malachite green and crystal violet) with complex structures, the degradation rate within 24 hours reaches 90% or above, and the method has wide application prospects in the aspect of treating refractory acid dye wastewater.

To determine the presence of Mycobacterium in an environment, a sample from the environment can be plated onto a growth medium that is selective for Mycobacterium. Prior to plating, the sample may be treated with a compound containing sodium dodecyl sulfate containing glycine hydrochloride. Plating may occur immediately after the addition of the compound or after a standing period. The agar based growth medium can include a high concentration of crystal violet, in excess of 0.5 μg / ml. Mycobacterium colonies will generally appear white while other colonies will generally appear stained purple or another color.

The invention provides a high-throughput screening method of an anti-Nipah virus drug. The method comprises the following steps: firstly, completing drug gradient dilution and planking pretreatment in a BSL-2 laboratory; then carrying out virus infection, cell culture and 72-hour incubation in the BSL-4 core region; the method comprises the following steps: carrying out rapid and low-cost visual preliminary screening on a whole-plate sample through crystal violet dyeing, and selectively carrying out TRIzol LS inactivation treatment on a small amount of potential effective sample supernatant; after the inactivated sample is transferred to a BSL-2 laboratory, the virus copy number is accurately quantified by adopting a qRT-PCR technology, and finally the drug inhibition ratio and the EC50 value are calculated. According to the method, through a staged screening strategy, on the premise of ensuring biological safety, the screening flux is greatly improved, the detection cost is reduced, and a reliable solution is provided for drug screening of high-pathogenicity pathogenic microorganisms.

This invention relates to the field of microbial mineralization technology, providing a method for rapidly determining whether bacteria can form a calcium carbonate protective layer on a steel surface. The method includes the following steps: S1, immersing at least two steel substrates in a test bacterial solution containing the bacteria to be tested, and incubating for 3 days; S2, detecting the calcium signal intensity on the substrate surface: taking at least one steel substrate and sequentially performing TPEN immersion treatment, calcium ion fluorescent probe immersion treatment, and room temperature incubation in the dark, then detecting the fluorescence signal intensity on the steel substrate surface; S3, detecting bacterial adhesion: taking at least one steel substrate and performing cleaning, crystal violet staining, cleaning, and immersion in anhydrous ethanol, then detecting the absorbance value of the anhydrous ethanol; S4, determining the ability of the test bacteria to form a calcium carbonate protective layer on the steel surface based on the fluorescence signal intensity and absorbance value. Compared to existing technologies, this invention, through the combined detection of "calcium signal at the steel substrate interface + bacterial adhesion," shortens the detection cycle by more than 57%.

The application discloses a Pseudomonas soli and application thereof. The Pseudomonas soli is Pseudomonas soli H19 with a preservation number of CGMCC No. 27823. The strain has a good flocculation effect on kaolin suspension and crystal violet dye solution. Experiments show that 2 mL of a microbial flocculant prepared by using the strain and an equal amount of coagulation aid of 0.1 mol / L FeCl2 can make the flocculation rates of 50 mL of 4 g / L kaolin and 20 mg / L crystal violet solution reach 87.26% and 97.34% respectively, and the microbial flocculant can be used for flocculation of industrial suspended particles and dye wastewater.

This invention discloses a three-dimensional cobalt metal-organic complex, its preparation method, and its applications, belonging to the field of crystal materials technology. This invention uses 2,5-diketopiperazine-N,N'-diacetic acid and 4,4'-bipyridine as raw materials with cobalt ions to prepare a compound with the chemical formula [Co(DPDA)(4,4'-bpy)]. n A three-dimensional cobalt-organic metal complex. It has a six-connected three-dimensional network structure with dual-core [Co2(COO)2] structural units, and its topological symbol is {3 12 0.4 24 0.5 9 Furthermore, the aforementioned three-dimensional cobalt metal-organic complex can rapidly activate peroxymonosulfate to effectively degrade dyes. It can degrade methylene blue, malachite green, and crystal violet to over 95.0% within 5 minutes, without producing toxic byproducts during the degradation process, and exhibits good reusability. Additionally, the synthesis conditions of the three-dimensional cobalt metal-organic complex are mild, the steps are simple, and it possesses excellent structural stability, good thermal stability, and antiferromagnetism at high temperatures.

The present application relates to the technical field of supramolecular chemistry, and particularly relates to a pyrimidine acylhydrazine organic gel factor and preparation and application thereof.The preparation process of the pyrimidine acylhydrazine organic gel factor is simple, and the organic gel prepared by further using the pyrimidine acylhydrazine derivative organic gel factor can effectively adsorb and remove bright green, malachite green, crystal violet, rhodamine B and other organic dyes.Among them, the removal rate of bright green dye can be as high as 99.86%, and the removal efficiency of rhodamine B dye can be as high as 99.89%;it is shown that the organic gel of the present application has excellent removal efficiency on organic dyes.

The invention relates to the technical field of microbiological detection, and particularly discloses a culture medium for selectively separating and culturing Burkholderia cepacia, which comprises the following components: 8-12 g / L of tryptone, 8-12 g / L of sucrose, 1-2 g / L of yeast extract, 1-2 g / L of monopotassium phosphate, 5-10 g / L of sodium pyruvate, 4-7 g / L of sodium chloride, 15-23 mg / L of phenol red, 10-18 g / L of agar, 0.8-1.3 mg / L of crystal violet, 0.5-5 g / L of selective inhibitor, and the balance of water. 10-30 g / L of a specific accelerant and the balance of water; the selective inhibitor is selected from one or more of sodium deoxycholate, sodium taurocholate, sodium glycocholate and propyl gallate; the specific accelerant is lactose, cane sugar and vanillic acid; and the culture medium does not contain any antibiotic component. The culture medium disclosed by the invention is free of antibiotic components, good in selectivity and high in growth promoting ability, and has a very good market application prospect.

This invention belongs to the field of nanocomposite materials and spectroscopic detection technology. This invention discloses an Ag / Co-Bi4Ti3O... 12 Composite SERS substrate, its preparation method, and its application in the detection of crystal violet molecules in water: First, cobalt-doped bismuth titanate (Co-Bi4Ti3O) was prepared using a hydrothermal-calcination method. 12 Ag / Co-Bi4Ti3O was prepared by loading silver nanoparticles onto the surface of a carrier using sodium citrate as a reducing agent. 12 Composite SERS substrate. This invention utilizes cobalt doping to modify and optimize Bi4Ti3O. 12 By combining the electromagnetic enhancement effect of localized surface plasmon resonance of silver nanoparticles with the chemical enhancement effect of semiconductor charge transfer, a dual synergistic SERS enhancement system is constructed, solving the problems of weak enhancement effect of traditional SERS substrates, easy aggregation of silver particles, and poor detection uniformity and cycling stability. This enables ultra-trace, rapid, and highly sensitive Raman detection of crystal violet in water.