320 results about "Crystal violet" patented technology

The invention especially relates to a Raman probe using a gold nanosol as a substrate for detection of mercury ions and a preparation method thereof, which belongs to the technical field of Raman spectrum detection. The preparation method comprises the following steps: adding secondary deionized water into a nano-silver dispersion liquid with phytic acid micelle as a protective agent, carrying out stirring, heating to a temperature of 40 to 60 DEG C, adding a perchloroauric acid solution drop by drop, carrying out stirring for 10 to 20 min, then adding trisodium citrate drop by drop and carrying out stirring for 10 to 20 min and cooling so as to prepare the gold nanosol; and adding crystal violet into the prepared gold nanosol and carrying out uniform mixing. The preparation method for the Raman probe is simple and convenient and needs short preparation time. A gold nanoparticle in the Raman probe has good stability; and the Raman probe has the advantages of capacity of rapid detection of mercury, high sensitivity, good selectivity and a mercury ion detection limit of 0.5*10<-12> mol / L.

The invention provides a pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels, belonging to the field of analytical chemistry. The method comprises the operations of weighing of a sample to be detected, homogenization, solid phase extraction, elution and the like; instruments used in the method comprise a constant temperature oscillator, a high speed dispersing homogenizer, a centrifugal machine and the like. A sample solution prepared by using the pretreatment method can be directly detected by using a liquid chromatogram-tandem mass spectrometry method; compared with the prior art, the pretreatment method has the advantages of simple and effective operation, less time consumption, low cost, good reproducibility and the like, and has very strong operability, so that the pretreatment method has the hope to be widely popularized and applied in a large scale in industries such as environment protection, commodity inspection, entry and exit inspection and quarantine and the like, with significant economic benefits.

The invention relates to bacillus thuringiensis for killing meloidogyn incognita and a culture method of the bacillus thuringiensis. The bacillus thuringiensis NBIN-863 for killing meloidogyn incognita is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan China on 29th November, 2013, the survival date is 29th November, 2013, and the serial number in the Center is CCTCC NO: M2013612. A culture method of the Bacillus thuringiensis comprises the following steps: taking soil at 117 degrees and 29 minutes east longitude and 30 degrees and 39 minutes north latitude near the Jiuhuashan in Anhui province, suspending in sterile water, diluting according to gradient concentration, coating on a flat plate, culturing by using an LB (Lusso-Brillante) solid culture medium, diluting, drawing a line on an LB flat plate for culture, inoculating a single colony to a shake flask for culture, mixing with glycerin, preserving in a refrigerator, transferring the single colony into a liquid culture medium for culture, smearing and observing and confirming by using a crystal violet staining optical microscope.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and / or paddy straw are / is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

The invention discloses a preparation method of nano-silver-carrying PNIPAM / PVA composite thermosensitive gel. PNIPAM / PVA thermosensitive hydrogel prepared with a free radical polymerization method is used as a reducing medium, and silver nanoparticles are reduced in a network structure of the hydrogel; the finally prepared material is used as the substrate of SERS and soaked in crystal violet probe molecular solutions with different concentrations for a certain time; then the substrate material with probe molecules adsorbed on the surface is taken out, placed on a slide for airing and used for Raman detection. The hydrogel in the gel is used as a dispersion stabilizer and can play a role in support protection for electromagnetically enhanced nano-silver particles; when the gel is adopted as the substrate for detecting crystal violet at the room temperature, the detection limit can reach 10<-9> orders of magnitude, and the fact that the intensity of a Raman signal varies with temperature is also found. The disclosed SERS substrate adopts simple preparation method and is good in temperature response and has broad application prospect in fields of food additives, pesticide detection, sensing and the like.

Simple carboxylic acids, in particular dicarboxylic acids such as citric acid shows an unexpected ability to enhance the antimicrobial power of a wide range of disinfectant and / or antibiotic agents. As little as 1% citrate greatly enhances the ability of antibiotics to kill or inhibit a wide range of bacterial species including antibiotic resistant strains. Citrate alone is effective in preventing bacterial growth in platelet concentrates and in red blood cell suspensions. Effective concentrations of citrate cause little if any damage to blood cells. Besides enhancing the power of antibiotics citrate also enhances the antimicrobial properties of disinfectant organic dyes such as crystal violet and methylene blue. In addition citrate enhances the antimicrobial properties of polyphenols of plant origin. Iodine-based disinfectants are also enhanced without enhancing protein denaturation.

The invention relates to a nitrogen-doped three-dimensional graphene loaded carbon coated copper substrate material and a preparation method. Nitrogen-doped three-dimensional graphene loaded carbon coated copper nanoparticles are directly synthesized with a one-step chemical vapor deposition method, the costs of raw materials used in preparation are low, the process is simple and easy to operate, and macro production is facilitated; the obtained carbon coated copper nanoparticles are subjected to in-situ synthesis and uniformly loaded on the surface of three-dimensional graphene, and the technical problem of non-uniform distribution of an exterior addition method is solved; the lower detection limit of the nitrogen-doped three-dimensional graphene loaded carbon coated copper substrate material after coating on probe molecule crystal violet can be up to 1*10 <-6> mol / L, and better stability is achieved; according to the method, the process is simple, and the substrate material prepared with the method has excellent detection effect.

The invention relates to a fermentation method of bacillus megatherium with the high rate of sporation. The method comprises the following steps that 1, the bacillus megatherium is inoculated into a solid medium after being activated, static culture is conducted, and secondary activated bacillus megatherium is obtained; 2, the secondary activated bacillus megatherium is inoculated into a seed medium, fermentation culture is conducted, and seed bacterial liquid is prepared; 3, the seed bacterial liquid is inoculated into a fermentation medium, fermentation culture is conducted, supplementary food liquid is added, fermentation is conducted continuously, and bacillus megatherium fermentation liquor is prepared. According to the fermentation method of the bacillus megatherium with the high rate of sporation, culture conditions and medium components are selected, and meanwhile sporation accelerants of bacillus megatherium are added in the fermentation process, the viable count and the rate of sporation of the bacillus megatherium are increased, and the viable count in the prepared fermentation liquor can reach above 8.0*109 CFU / ml; by means of oil lens observation after crystal violet staining, the rate of sporation is above 95 percent, the spore count of a product can be significantly increased, and the product quality of the product can be significantly improved.

The invention discloses a preparation method of nano-silver-loaded polyvinyl alcohol hydrogel. Sodium borohydride is adopted as a reductant, and the nano-silver-loaded polyvinyl alcoholhydrogel with controllable morphology and particle size is prepared with a cycle freezing and thawing method and an immersion method. The operation is simple, complicated technological equipment is not needed, and polyvinyl alcohol adopted as a dispersion stabilizer can have supporting and protecting effects on nano-silver particles with enhanced electromagnetism. When crystal violet is detected with the nano-silver-loaded polyvinyl alcohol hydrogel, the detection limit of the hydrogel can reach 10-12 orders of magnitude, and the hydrogel is convenient to use and easy to operate.

The invention discloses a pullorum staining agglutination antigen as well as a preparation method and an application thereof, and belongs to the technical field of veterinarian diagnosis. Salmonella pullorum SP7220, SP7701, SP8441 and SP9905 are subjected to recovery and passage respectively to form a seed bacterium liquid, then the seed bacterium liquid is inoculated with a solid culture medium for proliferation, after inactivation with formalin, the concentration of the bacterium liquid is adjusted with turbidimetry, finally, the bacterium is fully and evenly mixed and packaged after a crystal violet solution is added for staining, and the pullorum staining agglutination antigen is prepared. The technological method is simple, reasonable, scientific, stable in production and low in cost, the selected production stains of the pullorum staining agglutination antigen are good in antigenicity and low in mutation rate, the prepared pullorum staining agglutination antigen product has the advantages of high sensitivity, high specificity, quickness in diagnosis and clearness in agglutination image, the staining agglutination antigen is relatively ideal for pullorum detection.

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge of wild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth of the aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and the extra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

The invention discloses a method for coprocessing crystal violet polluted sewage by utilizing outdoor natural light-hydrogen peroxide. The method comprises the steps of: mixing and stirring photochemical catalyst and the sewage to be processed, and carrying out ultrasonic dispersion and adsorption equilibrium; adding the hydrogen peroxide; and carrying out outdoor natural light irradiation, filtering, separating, recovering the photochemical catalyst, and the like. By combining the hydrogen peroxide and the outdoor natural light, the method can generate hydroxyl free radical with strong oxidation capacity and has stronger oxidation capability for the high-concentration crystal violet printing and dyeing waste water which is difficult in biodegradation, thus achieving the aim of thoroughly purifying the crystal violet polluted sewage with high efficiency.

The invention discloses a catalytic material with a core-shell structure, gamma-Fe2O3-Fe3-xBixNbO7 (x is not less than 0.5 but not more than 1), SiO2-Fe3-xBixNbO7 (x is not less than 0.5 but not more than 1), MnO-Fe3-xBixNbO7 (x is not less than 0.5 but not more than 1); the particle size of gamma-Fe2O3, SiO2 and MnO is 0.06 to 2 microns, the particle size of core-coated Fe3-xBixNbO7 (x is not less than 0.5 but not more than 1) is 0.08 to 1.2 microns; the application of the catalytic material with the core-shell structure includes that: a reaction system consisting of a magnetic field device and photocatalytic material with a core-shell structure is used for degrading pentachlorophenol, Atrazine, diuron, crystal violet and other organic pollutants in water, and the magnetic-field intensity is chosen to be from 0.5 to 5T, and a xenom lamp or a high voltage mercury lamp is used as a light source; the volume percents of the three magnetic composite photocatalytic materials are all 1 / 3 of volume ratio so that the magnetic composite photocatalytic materials can be distributed in aqueous solution uniformly, and oxygenation and aeration are simultaneously adopted; the entire photoreaction is implemented under airtight and light-tight environment. Novel catalyst is loaded on a magnetic particle core through a multi-target magnetron sputtering deposition method, a pulse laser sputtering deposition method or a metal organic substance chemical vapor deposition method.

The invention discloses a preparation method and application of hapten and artificial antigen capable of being used for detecting crystal violet and malachite green together. The hapten is a compound shown in the formula (IV), and the antigen is formed by coupling the hapten with carrier protein and used for crystal violet and malachite green. The antigen and the antibody prepared through the method can be used for preparing enzyme-linked immunoassay kit. The method has the advantages that the production process is safe and simple, temperature requirements and conditions are not strict, a product is stable in quality, and cost is low; according to the hapten, the antigen and the corresponding antibody structure of the antigen, the prepared enzyme-linked immunoassay kit is high in sensitivity to malachite green and crystal violet, and the cross reaction rate reaches 95% or more.

The invention discloses catalytic materials with core-shell structures, i.e., gamma-Fe2O3-Y3-xBixNbO7 (0.5<=x<=1), SiO2-Y3-xBixNbO7 (0.5<=x<=1), and MnO-Y3-xBixNbO7 (0.5<=x<=1), wherein the particle size of gamma-Fe2O3, SiO2 and MnO is 0.06-2 microns, and the particle size of core-coated Y3-xBixNbO7 (0.5<=x<=1) is 0.08-1.2 microns; the applications of the catalytic materials with core-shell structures are as follows: a reaction system consisting of a magnetic field device and photocatalytic materials with core-shell structures is used for degrading pentachlorophenol, Atrazine, diuron, crystal violet and other organic pollutants, and the magnetic-field intensity is chosen to be from 0.5T to 5T, and a xenom lamp or a high voltage mercury lamp is used as a light source; the volume percents of the three magnetic composite photocatalytic materials are all 1 / 3 in volume ratio so that the magnetic composite photocatalytic materials can be distributed in an aqueous solution uniformly, an edge filter ((lambda) 420nm) is adopted and oxygenation and aeration are simultaneously adopted. A novel catalyst is loaded on a magnetic particle core through a multi-target magnetron sputtering deposition method or metal organic substance chemical vapor deposition method. Y3-xBixNbO7 (0.5<=x<=1) powder is used as a catalyst or Pt, NiO and RuO2 catalysts are loaded respectively.

The invention discloses a metal organic framework material. The framework material is formed by mutual bridging of an organic ligand and metal ions in a space, and has a chemical formula of [AmOn(BPTC)].xB, wherein m = 1 to 5, n = 1 to 5, A is one selected from the group consisting of manganese, copper, cobalt, nickel, iron and zinc, BPTC is a biphenyl tetracarboxylic radical, B is one selected from charged positive cations including C2N<+>, H3O<+>, NH4<+>, Li<+>, Na<+>, K<+>, Mg<+>, Ca<+> and Al<+> or one selected from positively-charged macromolecules including methylene blue, methyl green-pyronin, rhodamine B, methyl green, crystal violet, magenta and malachite green, and X is 1 to 100. The invention also discloses a method of synthesizing the above metal organic framework material andan application of the metal organic framework material. According to the metal organic framework material, synthetic method and application provided by the invention, the metal organic framework material has the advantages of being capable of adsorbing, desorbing and recycling a large amount of enriched heavy metals and dyes.

The preparation process of cow mammitis streptococcus culture fluid and its use belongs to the field of animal epidemic disease diagnosing, preventing and treating technology. The preparation process includes dissolving peptone 10 g, yeast powder 5 g, sodium chloride 10 g, glucose 5 g and sodium azide 0.5 g in water of 1 L; regulating pH to 7.2 with sodium hydroxide; adding bacteria-free 0.1% concentration crystal violet water solution in 1.3ml and 1% concentration bromocreasol purple water solution in 1.5ml; sterilizing at 121deg.c and preservation at 4 deg. c. The culture fluid may be used in the fast identification of cow mammitis streptococcus and antibiotic sensitivity test.

The invention discloses a method for efficiently removing typical triphenylmethane dye from water, and belongs to the field of wastewater treatment. Microwave is utilized to excite an electrodeless lamp to generate ultraviolet-visible light to respectively initiate TiO2 photo-catalysis decomposition and H2O2 non-catalytic oxidation decomposition of malachite green and crystal violet typical triphenylmethane dye wastewater. The used microwave radiation is generated by a microwave generation device, wherein the microwave generation device mainly comprises a microwave power source (1), a reaction chamber (13), a glass reactor (15), the electrodeless lamp (16), a condenser pipe (19) and a temperature probe (21). By improving a conventional microwave chemical reaction device, the reaction device can work for a long time, accurately measure the temperature of the reaction system, the emission spectrum of the electrodeless lamp and the like, and treat the malachite green wastewater and the crystal violet wastewater caused by the triphenylmethane dye; and thus the method has the advantages of high efficiency, simple process, strong controllability, mild reaction conditions, simple and convenient operation and the like.

The invention discloses a method for rapidly detecting malachite green or crystal violet in aquaculture water, which comprises the steps of using a rapid synergic solvent extraction system formed by auxiliary extraction agent, extraction agent and water to extract the crystal violet or the malachite green into organic phase at the upper part of a centrifuge tube; and detecting the malachite green or the crystal violet in the sample by comparing the standard content of the malachite green or the crystal violet, wherein the limit of detection is 0.05mu g / mL. Compared with the existing detection methods, the method is mild in extraction conditions and rapid in detection; the whole analysis and detection process can be completed within 5-10min; and the method is low in cost, does not need a large instrument and is high in accuracy, thus being suitable for detecting the safety of edible aquatic products and having great utilization potentiality and wide market prospect.

The present invention provides a method for measuring 3, 5-substituted oxazolidone compound content, it is non-water detection method and includes: dissolving the sample by adding acid; adding crystal violet instruction liquid to the dissolved solution; titrating perchloric acid titrating solution to the solution till the solution becomes blue-green; and calculating the content of the sample. The method is convenient in the operation for the content measurement for chemical material medicine and content measurement for comparison sample, has accurate measuring result, and is simple in execution.

The invention discloses a preparation method and an application of Fe3O4@PPy@Au magnetic composite microspheres. Fe3O4 nanoparticles are synthesized firstly and covered with PPy layers, then synthesis is controlled with a two-step method for seed growing, and accordingly, the surface coated particles are relatively complete; used raw materials are easily available, a process is simple, ultrasonic-assisted synthesis is used, and high dispersity of the composite microspheres is guaranteed; sizes of the Au particles on the surfaces are controlled very well by controlling potassium carbonate and ascorbic acid; the prepared Fe3O4@PPy@Au magnetic nanoparticles have magnetism of Fe3O4 and Raman enhancement performance of the nano-Au particles. The Fe3O4@PPy@Au magnetic nanoparticles are taken as an SERS (surface-enhanced Raman spectroscopy) substrate and dispersed in a crystal violet solution with certain concentration, crystal violet can be enabled to be adsorbed and enriched to the surfaces of the magnetic particles initiatively, and Raman characteristic peaks of the crystal violet can be detected within short time through SERS enhancement.

The invention discloses immobilized spore laccase as well as a preparation method and an application thereof, and belongs to the immobilized technical field and the wastewater treatment field. The immobilized spore laccase is formed by immobilizing spore laccase produced by a bacillus bacterial strain on DEAE(diethyl-aminoethanol)-cellulose through an ionic bond and a hydrogen bond, and has the extensive pH adaptive capacity and the heat resistnace of the spore laccase; in a solution containing an organic solvent, metal ions or an inhibitor, the immobilized spore laccase has stability which is stronger than that of free spore laccase. The preparation method of the immobilized spore laccase mainly comprises four steps of spore preparation, carrier pre-treatment, adsorption and collection, is simple and convenient to operate, and high in immobilization rate. The immobilized spore laccase provided by the invention has very strong dye discoloring capacity and has a highest discoloring rate up to about 95% while being applied to spinning dyes of methyl green, methyl red, cid red 1 and crystal violet. The carrier also can selectively adsorb the dyes to form complementary action with laccase.

The invention relates to a preparation method of drilling core containing acid liquor indicator, which comprises the following components as calculated by the total weight 100% of the drilling core: 14% of epoxy resin, 2% of quadrol, 2% of acetone and 1% of methyl red. The preparation method has the following steps: mixing the above components; weighting the rock waste raw materal accounting for 14% of the total weight of the drilling core, and washing an performing oil treatment on the raw material by petroleum ether; drying for 3h under the temperature of 95 DEG C, cooling and adding 1% crystal violet; stirring a cementing agent with the rock waste raw material evenly, placing the mixture in a die and pressurizing by 7MPa-10MPa for 12h; and dismantling the die, drying the drilling core for 6h at the temperature of 95 DEG C, and naturally cooling to room temperature. The permeability, water sensitivity, salt sensitivity, velocity sensitivity, acid sensitivity and alkali sensitivity of the drilling core in the invention are similar to those of the natural core, which is proved by a stepped permeability tester and five-sensitivity tests; and the drilling core has good indicating effect on acid liquor.

The invention discloses a method for removing a crystal violet dye in water by adopting a sludge-based biomass hydrothermal carbon adsorbent and belongs to the field of water treatment. The method comprises the steps of centrifuging sludge, carrying out preliminary dehydration, drying, grinding and sieving until the particle sizes are 350-850 microns; weighing 25-100g of sludge particles, adding 400-475ml of water to prepare a sludge-water mixed solution; adjusting the pH value of the sludge-water mixed solution to be 5-9 by using HCl and NaOH solutions, putting the mixed solution into a reaction kettle, covering a reaction kettle cover, introducing nitrogen into the reaction kettle for 3min and forming an oxygen-free environment in a kettle body; reacting at 180-240 DEG C for 3-9h to generate sludge-based biomass hydrothermal carbon; and centrifuging and cleaning by using water for multiple times, and drying to obtain the sludge-based biomass hydrothermal carbon adsorbent. The method is simple in operation, low in cost and free of secondary pollution, and has an industrialization prospect. The environmental load is reduced, meanwhile, a new way is provided for utilization of municipal sludge, and resource utilization of the sludge and water environment protection are achieved.

The invention discloses a rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease, which comprises the following steps: A) taking konjac tissues infected by the soft rot disease; B) inoculating the tissues at the interface of diseased and healthy tissues onto a nutrient agar culture medium after carrying out surface disinfection; C) streaking the bacteria grown out on the sides of the tissues, and selecting a single colony for purification and preservation; D) adopting a distinctive Erwinia crystal violet polypectin selective culture medium (CVP) to screen the pathogens of the konjac soft rot disease; E) adopting the point connection method to carry out primary screening of antagonistic bacteria; and F) adopting the oxford cup method to carry out secondary screening of the antagonistic bacteria. The method has simple operation and easy operation, and can rapidly and effectively screen out the antagonistic bacteria against the pathogens of the konjac soft rot disease and simultaneously provide a concept for rapid screening of the antagonistic bacteria against other crop diseases.

The invention provides a surface enhancement Raman scattering (SERS) substrate and a preparation method of same. The SERS substrate employs cetyl trimethyl ammonium bromide (CTAB) as a morphology controlling agent and three-dimensional macroporous foamed nickel as a base material, is prepared through a replacement reaction of chloroauric acid and nickel, and has abundant high-enhancement-activity gold micro-nano conical spike-shaped structures which are uniformly distributed. The preparation method includes the steps of: under the effect of the CTAB, performing a reaction to the chloroauric acid and nickel at 20-30 DEG C for a certain period. The SERS substrate has a unique and uniformly distributed gold micro-nano cone-shaped array structure and a three-dimensional macroporous structure, has high enhancement performance of probe molecule Raman signals and has good repeatability in the enhanced signals. The SERS substrate can be used for high-sensitive surface enhanced Raman spectroscopy detection for dye molecules, such as crystal violet, malachite green, methylene blue and the like in environment. The preparation method is simple, is low in cost, is free of special instrument and has good repeatability.

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

The invention discloses a diatomite / nano TS-1 titanium silicalite composite material with a hierarchical porous structure and a preparation method of the diatomite / nano TS-1 titanium silicalite composite material. By adoption of the cheap diatomite as a carrier, nano TS-1 titanium silicalite is uniformly loaded on the surface of the diatomite by means of electrical property inversion, adsorption, nucleation and crystallization to obtain the diatomite / nano TS-1 titanium silicalite composite material with a hierarchical porous structure having macropores / mesopores / micropores, and the composite materials is large in specific surface area and total pore volume. By loading of the nano TS-1 titanium silicalite on the surface of the diatomite, problems of single pore structure and low specific surface area of the diatomite can be solved, the obtained composite materials is excellent in photocatalytic degradation of organic pollutants such as crystal violet, and application value of the diatomite in the field of catalysis is increased. In addition, the preparation method of the diatomite / nano TS-1 titanium silicalite composite material has advantages of technical simplicity and feasibility, low cost, low energy consumption and low pollution and has a promising industrial application prospect.

The invention discloses a gold-silver nano cone array with body-enhanced Raman scattering effect as well as a preparation method thereof and use thereof. The array is a nano cone ordered array with acone bottom diameter of 500-520 nm, a cone top diameter of 115-125 nm, bone height of 465-475 nm and spacing less than or equal to 10 nm between adjacent two cone bottoms, wherein the cone is formed by coating a silver nano film outside a gold nano film, and the cone is arranged on a flat plate shaped polymethyl methacrylate substrate. The method comprises the following steps: laminating a hexagonally arranged nano pillar array nickel coining die and an aluminum sheet, performing multi-time anode oxidizing and wet-process etching on the aluminum sheet with concave marks, evaporating a gold film on an obtained inverted-cone-shaped porous aluminum oxide template, coating a polymethyl methacrylate solution on the aluminum oxide template; etching off the aluminum oxide template which is sequentially coated with the gold film and polymethyl methacrylate in the inverted-cone-shaped porous aluminum oxide template, and then, sputtering a silver film on a gold nano cone ordered array on the polymethyl methacrylate substrate to obtain a target product. The gold-silver nano cone array can be used for trace detection of rhodamine or crystal violet or malachite green on a body-enhanced Raman scattering active substrate.

The invention discloses aerobic active marine bacteria and a preparation method of a decolorizing flocculant of the aerobic active marine bacteria. The aerobic active marine bacteria is formed by performing isolation and screening on mud disgorged by ruditapes philippinarum bred in a sea farm in a Zhujiajian, Zhoushan, Zhejiang in China, and is authenticated to be pseudoalteromonas ((i) Pseudoalteromonas ( / i)sp.). The decolorizing flocculant has the beneficial effects that the formed adecolorizing flocculant has good decoloration effect, and the flocculation rate of the decolorizing flocculant to kaolin reaches greater than 90 percent; the decolorizing flocculant has high activity for performing decoloration on high-concentration waste water, and the flocculation rate of the decolorizing flocculant to methylene blue, malachite green and crystal violet reaches greater than 92 percent, 99 percent and 98 percent; the decolorizing flocculant does not have potential toxicity for organisms, and is green and healthy.