Method and composition for staining and processing a urine sample

A composition and sample technology, applied in the preparation of test samples, analysis of suspensions and porous materials, sampling, etc., can solve problems such as difficult images and damaged sediments

Active Publication Date: 2015-11-25
IRIS INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, urinary sediments are diverse in their morphology and many sediments are damaged, and thus, it is difficult to perform determination with good accuracy on the acquired images
Especially difficult to determine with good precision small size sediments such as red blood cells (especially heterogeneous red blood cells), bacteria and crystals without external user verification

Method used

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  • Method and composition for staining and processing a urine sample
  • Method and composition for staining and processing a urine sample
  • Method and composition for staining and processing a urine sample

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Embodiment Construction

[0052] The present invention relates to surprising and unexpected particulate contrast agent compositions for rapidly generating visual distinctions in fluid samples such as urine samples. The particulate contrast agent composition may be particularly useful in automated flow cytometric analysis systems. The particle contrast agent composition is composed of a combination of particle contrast agent and permeabilizing agent. Particulate contrast agent compositions may include fixatives and / or antimicrobial agents. In one embodiment, the particulate contrast agent composition is a mixture of crystal violet, saponin and Proclin300. In one embodiment that is surprisingly effective, crystal violet is present in an amount sufficient to result in a concentration of about 0.57 mM, saponin is present in an amount sufficient to result in a concentration of about 23.3% by weight, and Proclin 300 is present in an amount sufficient to result in a concentration of about 0.05 mM under stain...

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Abstract

The present disclosure relates to a staining methodology employing a particle contrast agent composition capable of rapidly staining cells in a single step. The particle contrast agent composition can be comprised of a combination of one or more particle contrast agents and one or more permeabilizing agents, optionally including one or more fixing agents and other components. The particle contrast agent composition can include Crystal Violet, 5PD-Lytic, and Proclin 300.

Description

[0001] Cross references to related patent applications [0002] This patent application claims the benefit of U.S. Patent Application No. 61 / 799,014, entitled "Analysis of Particles in Urine Samples," filed March 15, 2013, which is hereby incorporated by reference in its entirety incorporated. technical field [0003] The present invention relates generally to particulate contrast agents and more particularly to particulate contrast agent compositions for use in fully automated or semi-automated equipment to differentiate and quantify particles in fluid samples such as urine samples. Background technique [0004] Urine sediment analysis is one of the most commonly performed diagnostic tests to provide an overview of a patient's health status. A urine sample is obtained from the patient and stored in a test tube for later processing and analysis. The presence of certain characteristic sediments (also known as formed elements) in a urine sample may be clinically significant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N33/50
CPCG01N1/30G01N15/1459G01N15/1463G01N33/50G01N2015/0046G01N2015/1006G01N2015/1497G01N15/06G01N2001/302
Inventor J·科瑞明斯
Owner IRIS INT
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