Method for treating dye waste water by enzyme production through mixed biomass fermenting

A technology for biomass fermentation and dye wastewater, applied in the field of environmental treatment, can solve the problems that the advantages cannot be effectively exerted, and it is not conducive to optimizing the enzymatic hydrolysis efficiency of lignin-degrading enzymes, and achieves the best decolorization effect, high enzymatic hydrolysis efficiency, high enzymatic hydrolysis efficiency, etc. simple method effect

Inactive Publication Date: 2012-07-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the advantages of different agricultural waste components in inducing enzyme production cannot be effectively utilized, which is not conducive to optimizing the composition of lignin-degrading enzyme systems and improving their enzymatic hydrolysis efficiency

Method used

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  • Method for treating dye waste water by enzyme production through mixed biomass fermenting
  • Method for treating dye waste water by enzyme production through mixed biomass fermenting
  • Method for treating dye waste water by enzyme production through mixed biomass fermenting

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: The method for treating dye wastewater by using mixed biomass fermentation to produce enzymes, the specific steps are as follows:

[0033] (1) Trametes trogii ATCC 200800 Cultivation of Seeds

[0034] Inoculate the hyphae of Trametes trichotilloides on the wort medium (MEA), cultivate at 30°C for 8 days to obtain slant seeds, inoculate the activated slant seeds on the wort medium, and cultivate at 30°C for 6 days to obtain solid fermented seeds;

[0035] (2) Enzyme production by fermentation

[0036] Inoculate the solid fermented seeds into the solid fermentation substrate, inoculate 4 pieces of bacteria blocks (diameter of the bacteria block is 1cm) per 5g of the substrate, and cultivate at 35°C for 7 days;

[0037] The composition of the enzyme-producing solid fermentation substrate is 2% by mass of malt extract, 13mM copper sulfate, 20% by mass of a mixture of cottonseed hulls and rice straw (the mixing ratio of cottonseed hulls and rice stalks is ...

Embodiment 2

[0047] Embodiment 2: This method of utilizing mixed biomass fermentation to produce enzymes to treat dye wastewater, the specific steps are as follows:

[0048] (1) Trametes trogii ATCC 200800 Cultivation of Seeds

[0049] Inoculate the hyphae of Trametes trichotillomyces on malt extract medium (MEA), cultivate at 35°C for 10 days to obtain slant seeds, inoculate the slant seeds on wort medium, and cultivate at 35°C for 4 days to obtain solid fermented seeds;

[0050] (2) Enzyme production by fermentation

[0051] Inoculate the solid fermentation seeds into the solid fermentation substrate, inoculate 4 pieces of bacteria blocks (diameter of the bacteria block is 1cm) per 5g of the substrate, and incubate at 35°C for 21 days;

[0052] The composition of the enzyme-producing solid fermentation substrate is 2% by mass of malt extract, 13mM copper sulfate, 20% by mass of a mixture of cottonseed hulls and rice straw (the mixing ratio of cottonseed hulls and rice stalks is show...

Embodiment 3

[0061] Embodiment 3: The method for treating dye wastewater by using mixed biomass fermentation to produce enzymes, the specific steps are as follows:

[0062] (1) Trametes trogii ATCC 200800 Cultivation of Seeds

[0063] Inoculate the mycelium of Trametes trichotilloides on the slant of potato dextrose agar, cultivate at 37°C for 6 days to obtain slant seeds, inoculate the slant seeds on the slant of potato dextrose agar, and cultivate at 37°C for 2 days to obtain solid fermented seeds;

[0064] (2) Enzyme production by fermentation

[0065] Inoculate the solid fermented seeds into the solid fermentation substrate, and inoculate 4 pieces of bacteria blocks (diameter of the bacteria block is 1cm) per 5g of the substrate, and cultivate at 37°C for 15 days;

[0066] The composition of the enzyme-producing solid fermentation substrate is 2% by mass of malt extract, 13mM copper sulfate, 0.5% by mass of a mixture of cottonseed hulls and rice straw (mixed at a mass ratio of 1:1)...

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Abstract

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and/or paddy straw are/is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

Description

technical field [0001] The invention relates to a method for treating dye wastewater by fermenting mixed biomass to produce enzymes, and belongs to the field of environmental treatment. Background technique [0002] Synthetic dyes such as Congo red, phenol red, bromophenol blue, crystal violet and malachite green are common dyes in biological laboratories, and are widely used in the staining of cell characteristic structures, the study of physiological and biochemical characteristics of enzymes, and the study of toxicology of cells and individuals. They have stable chemical properties and have extremely strong "three causes" effects of teratogenicity, carcinogenicity and mutagenicity. If the dye sewage is discharged into a neutral environment without treatment, it can cause changes in the pH of the water body, increase in COD and TOC, and even lead to ecological crises. The dye absorbs sunlight and reduces the transmittance of the water body, causing the sunlight to be unab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F103/30
Inventor 严金平牛杰振伊日布斯
Owner KUNMING UNIV OF SCI & TECH
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