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Pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels

A malachite green and crystal violet technology, applied in the field of analytical chemistry, can solve the problems of low extraction efficiency, long processing time, and large loss of repeated extraction, achieving good reproducibility, low cost, and saving analysis time

Inactive Publication Date: 2013-03-20
FUZHOU UNIV
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Problems solved by technology

The key disadvantages of this type of pretreatment technology are low extraction efficiency, large loss in repeated extraction, and long processing time

Method used

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  • Pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels
  • Pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels

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Embodiment 1

[0026] The specific operation process of the pretreatment method for the detection of malachite green, crystal violet and their residues in eel of the present invention includes operations such as sample homogenization, solid phase extraction, and elution.

[0027] Specific steps:

[0028] (1) Accurately weigh 5.00 g (accurate to 0.01 g) of sample into a 50 mL centrifuge tube, add acetonitrile organic mixed solvent (20 mL of acetonitrile + 20 mL of glacial acetic acid), break up the sample with a high-speed dispersing homogenizer, add 4.0 g of basic alumina powder, shake for 1min, centrifuge at 4500rpm / min for 10min;

[0029] (2) Take 10mL supernatant and dilute it with 15mL high-purity water for later use to prepare 25mL sample solution;

[0030] (3)C 18 Solid phase extraction column (SPE cartridge C 18 -200mg / 3mL) activation process:

[0031] a. 2mL 2% glacial acetic acid / acetonitrile rinse;

[0032] b. 5mL 20% glacial acetic acid / methanol to remove...

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Abstract

The invention provides a pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels, belonging to the field of analytical chemistry. The method comprises the operations of weighing of a sample to be detected, homogenization, solid phase extraction, elution and the like; instruments used in the method comprise a constant temperature oscillator, a high speed dispersing homogenizer, a centrifugal machine and the like. A sample solution prepared by using the pretreatment method can be directly detected by using a liquid chromatogram-tandem mass spectrometry method; compared with the prior art, the pretreatment method has the advantages of simple and effective operation, less time consumption, low cost, good reproducibility and the like, and has very strong operability, so that the pretreatment method has the hope to be widely popularized and applied in a large scale in industries such as environment protection, commodity inspection, entry and exit inspection and quarantine and the like, with significant economic benefits.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a pretreatment method for detecting malachite green, crystal violet and residues thereof in eels. Background technique [0002] Malachite green (Malachite green) and crystal violet (Crystal violet) are a class of synthetic triphenylmethane industrial dyes, which have been widely used as an in vitro insect repellant, fungicide and disinfectant since the 1930s in aquaculture. In particular, it is very effective in the treatment of proliferative kidney disease (PKD) in fish. When malachite green and crystal violet are absorbed by the fish body, they are mainly metabolized into colorless malachite green and colorless crystal violet, respectively, and deposited in the fat tissue. Since animal experiments have proved that malachite green and crystal violet have potential teratogenic, carcinogenic and mutagenic effects on humans and animals, the European Union and t...

Claims

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Application Information

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IPC IPC(8): G01N30/08
Inventor 邱彬佘丽双林振宇郭隆华陈国南
Owner FUZHOU UNIV
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