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Bacillus thuringiensis for killing meloidogyn incognita and culture method of bacillus thuringiensis

A technology of root-knot nematode incognita and Bacillus chrysogenum, applied in the direction of nematicides, microbial-based methods, biochemical equipment and methods, etc., can solve problems such as polluting the environment

Active Publication Date: 2014-07-02
HUBEI BIOPESTICIDE ENG RES CENT
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Problems solved by technology

Most of the chemical agents are highly toxic drugs, which seriously pollute the environment

Method used

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  • Bacillus thuringiensis for killing meloidogyn incognita and culture method of bacillus thuringiensis
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  • Bacillus thuringiensis for killing meloidogyn incognita and culture method of bacillus thuringiensis

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Embodiment Construction

[0019] Bacillus thuringiensis strain Bacillus thuringiensis NBIN-863 that kills root-knot nematode incognita, depository unit: China Collection Center for Type Cultures, address: China, Wuhan, Wuhan University, collection center number CCTCC NO: M2013612, deposit date: November 27, 2013 Date, date of survival: November 29, 2013.

[0020] Morphology of the Bacillus thuringiensis strain that kills root-knot nematode incognita: the single colony grown on the plate is milky white snowflake-like, with a rough surface, and it is rod-shaped under the electron microscope after 24 hours of growth. After 36 hours of growth, it becomes unstained spores and stained spores Rhombic crystals; 6 proteins were produced in the supernatant after 48 hours of growth, 3 of which were insecticidal crystal proteins, namely Cry9Aa protein (130kDa), Cry2Aa protein (69kDa) and Cry1Ac protein (130kDa) ( image 3 ). Bacillus thuringiensis has activity against M. incognita.

[0021] On May 10, 2012, the ...

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Abstract

The invention relates to bacillus thuringiensis for killing meloidogyn incognita and a culture method of the bacillus thuringiensis. The bacillus thuringiensis NBIN-863 for killing meloidogyn incognita is preserved in the China Center for Type Culture Collection in Wuhan University in Wuhan China on 29th November, 2013, the survival date is 29th November, 2013, and the serial number in the Center is CCTCC NO: M2013612. A culture method of the Bacillus thuringiensis comprises the following steps: taking soil at 117 degrees and 29 minutes east longitude and 30 degrees and 39 minutes north latitude near the Jiuhuashan in Anhui province, suspending in sterile water, diluting according to gradient concentration, coating on a flat plate, culturing by using an LB (Lusso-Brillante) solid culture medium, diluting, drawing a line on an LB flat plate for culture, inoculating a single colony to a shake flask for culture, mixing with glycerin, preserving in a refrigerator, transferring the single colony into a liquid culture medium for culture, smearing and observing and confirming by using a crystal violet staining optical microscope.

Description

technical field [0001] The invention relates to a bacillus thuringiensis for killing root-knot nematode incognita and a cultivation method thereof. Background technique [0002] According to a conservative estimate by FAO, the annual loss of food and fiber crops due to nematode damage is about 12%, and the loss of vegetables, peanuts, tobacco and some fruit trees is more than 20%, or even 50%. According to reports by authoritative experts, plant parasitic nematodes cause about US$150 billion in losses in world agricultural production every year. The annual loss caused by nematodes in our country is about 3.5 billion US dollars. [0003] At present, there are more than 3,000 kinds of nematodes that are harmful to plants, and more than 40 kinds have been found in my country. One of the nematodes that is most harmful to crops is root-knot nematode. Root-knot nematodes are tiny and live in plants or in the soil. They will be transferred with rain, labor operations and diseased...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P5/00C12R1/07
Inventor 刘晓艳黄大野杨自文闵勇王开梅万中义曹春霞周荣华江爱兵
Owner HUBEI BIOPESTICIDE ENG RES CENT
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