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33 results about "Methyl green" patented technology

Methyl green (CI 42585) is a cationic or positive charged stain, related to Ethyl Green, that has been used for staining DNA since the 19th century. It has been used for staining cell nuclei either as a part of the classical Unna-Pappenheim stain, or as a nuclear counterstain ever since. In recent years, its fluorescent properties when bound to DNA have positioned it useful for far-red imaging of live cell nuclei. Fluorescent DNA staining is routinely used in cancer prognosis. Methyl green also emerges as an alternative stain for DNA in agarose gels, fluorometric assays and flow cytometry. It has also been shown that it can be used as an exclusion viability stain for cells. Its interaction with DNA has been shown to be non-intercalating, in other words not inserting itself into the DNA, but instead electrostatic with the DNA major groove. It is used in combination with pyronin in the methyl green–pyronin stain which stains and differentiates DNA and RNA.

Method for measuring content of trace cadmium in rice by utilizing flame atomic absorption spectrometry

The invention provides a method for measuring the content of trace cadmium in rice by utilizing a flame atomic absorption spectrometry. The method is characterized by comprising the following steps of preprocessing a rice sample by utilizing a microwave digestion manner in which a long-time soaking manner is combined with a step type heating manner, after the digestion, reacting cadmium ions with potassium iodide to generate CdI4<2->, reacting the CdI4<2-> with methyl green (MG) to generate a hydrophobic ionic association compound, extracting the hydrophobic ionic association compound into a micelle phase of a TritonX-114 nonionic surfactant, and measuring the cadmium content in the micelle phase by utilizing the flame atomic absorption spectrometry. The method solves the problems that the measurement of the trace cadmium by utilizing a traditional flame atomic absorption spectrometry is low in sensitivity and is easily interfered by matrixes and has the advantages of high precision and recovery rate, small relative standard deviation, accuracy, reliability, rapidness and convenience.
Owner:SUZHOU GUOHUAN ENVIRONMENT DETECTION

Sulfide detection method and device

The invention relates to a sulfide detection method and device. A sulfide is acidized to be converted into hydrogen sulfide so as to be separated from the complex matrix; the inert gas (nitrogen) serves as a carrier gas to carry generated hydrogen sulfide out and to enable hydrogen sulfide to enter a gas passage; when hydrogen sulfide meets filter paper which is placed in the gas passage and adsorbing a reagent (methyl green) that has selective response to hydrogen sulfide, the released hydrogen sulfide gas is gathered on the filter paper and react with methyl green; after reaction, methyl green on the filter paper turns from dark green to light green or colorless. The intensity of an optical signal is recorded in real time, the sulfide measurement process is simplified, and the interference on measurement from the complex matrix in which the endogenous sulfide is located is reduced. As the hydrogen sulfide gas is gathered on the filter paper, the detection limit is lowered, the detection sensitivity is improved and the relative running cost is low.
Owner:SHAANXI NORMAL UNIV

Immobilized spore laccase as well as preparation method and application thereof

The invention discloses immobilized spore laccase as well as a preparation method and an application thereof, and belongs to the immobilized technical field and the wastewater treatment field. The immobilized spore laccase is formed by immobilizing spore laccase produced by a bacillus bacterial strain on DEAE(diethyl-aminoethanol)-cellulose through an ionic bond and a hydrogen bond, and has the extensive pH adaptive capacity and the heat resistnace of the spore laccase; in a solution containing an organic solvent, metal ions or an inhibitor, the immobilized spore laccase has stability which is stronger than that of free spore laccase. The preparation method of the immobilized spore laccase mainly comprises four steps of spore preparation, carrier pre-treatment, adsorption and collection, is simple and convenient to operate, and high in immobilization rate. The immobilized spore laccase provided by the invention has very strong dye discoloring capacity and has a highest discoloring rate up to about 95% while being applied to spinning dyes of methyl green, methyl red, cid red 1 and crystal violet. The carrier also can selectively adsorb the dyes to form complementary action with laccase.
Owner:JIANGNAN UNIV

Fade resisting Miao embroidery raw material dye and dyeing method thereof

InactiveCN107119468AAnti-corrosion and efficientEfficient antifreezeDyeing processRetention timePhotochemistry
The invention discloses fade resisting Miao embroidery raw material dye and a dyeing method thereof. A formula of the fade resisting Miao embroidery raw material dye is prepared from 45 to 60 parts of dye and 28 to 47 parts of an auxiliary agent. The dyeing method of the fade resisting Miao embroidery raw material dye comprises the following steps: S1, preparing an original piece; S2, preparing colorization; S3, blending dye; S4, colorizing cloth; S5, carrying dye fixing on the cloth; S6, dehydrating the cloth. The dye disclosed by the invention is prepared from seaweed meal, fish meal, herba houttuyniae, haematine and methyl green, and the auxiliary agent is prepared from a preservative, an anti-settling agent, a wetting agent and a mordant, so the cloth rendered by the dye has the effects of high efficiency, freeze resistance, corrosion resistance and good colorizing effect, and the color and luster retention time of an original Miao embroidery piece is long; in the dye fixing process of the cloth, repeated rolling compaction is needed and water temperature is continuously reduced during rolling compaction, so that the cloth cannot easily fade after the cloth is colorized and can keep bright color for a long time; the dye is worthy of being popularized.
Owner:贵州绣之魂民族服饰有限公司

Method of detecting microorganisms and application thereof

The invention provides a method of detecting microorganisms and belongs to the technical field of quick diagnosis of nucleic acid. The method includes the steps of: 1) extracting nucleic acid from a sample to obtain a nucleic acid template; 2) mixing the nucleic acid template with a staining group and PCR buffer to obtain a mixed sample; 3) heating the mixed sample for degeneration or performing areaction with a non-specific nucleic acid dye to obtain a stained sample; 4) measuring color value and according to change of shade of the color, measuring the content of the nucleic acid and determining negative or positive of the microorganisms; herein, the staining group is a modified probe or a non-specific nucleic acid dye, wherein the probe includes a specific detection probe and a random detection probe; the non-specific nucleic acid dye includes: methyl blue, methyl green, xanthylium, SYBR Green, Ultrapure ethidium bromide, or Accublue fluorescein. The method is free of amplificationof an enzymatic reaction and can form a detection result within 5-10 min, is high in sensitivity and has good specificity.
Owner:INNER MONGOLIA HUAXING KANGWEI BIOTECH CO LTD

Natural low-carbon dye

The invention discloses a natural low-carbon dye which is composed of, by weight, 14-16 parts of basic fuchsin, 23-26 parts of methyl green, 31-33 parts of basic azaleine, 10-16 parts of sodium citrate, 13-18 parts of ferric ion, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of kudzu powder, and 9-10 parts of houttuynia cordata. The natural low-carbon dye is natural and odor-free, can quickly coloring an object and is not liable to discolor.
Owner:王世榕

Method for determining detergent in electroplating wastewater by methyl green photometry

The invention relates to a method for determining a detergent in electroplating wastewater by methyl green photometry. The method is characterized by including the following steps of: (1) preparing a methyl green water solution with a concentration of 2.5g / L, a buffer solution, chloroform, an oscillator, an ultraviolet / visible spectrophotometer, a separating funnel, and a volumetric flask; (2) using chloroform for first extraction; (3) using chloroform for secondary extraction; and (4) utilizing the ultraviolet / visible spectrophotometer to determine the detergent content. By utilizing the methyl green water solution for determining the detergent content in the electroplating wastewater, the method of the invention makes the determined deviation less than 6% and the sample solution labeling recovery rate range from 92.9% to 103%, and is characterized by simplicity, accuracy and reliability.
Owner:SUZHOU GUOHUAN ENVIRONMENT DETECTION

Environment-friendly dye

The invention discloses an environment-friendly dye. The environment-friendly dye is prepared from the following ingredients in parts by weight: 14 to 16 parts of methylene blue, 23 to 26 parts of methyl green, 31 to 33 parts of basic fuchsin, 10 to 16 parts of sodium citrate, 13 to 18 parts of ferric ion, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed solution, 21 to 24 parts of arrowroot flour and 9 to 10 parts of herba houttuyniae. The environment-friendly dye disclosed by the technical scheme of the invention has theadvantages of naturalness, no peculiar smell, quickness in coloring and no easiness in fading.
Owner:启东市中燕农副产品专业合作社

Dansyl derivative fluorescent probe as well as synthesis method and application thereof

The invention belongs to the technical field of organic synthesis and biological mercaptan detection, and particularly relates to a dansyl derivative fluorescent probe as well as a synthesis method and application thereof. The synthesis method of the fluorescent probe comprises the steps: dropwise adding a trichloromethane solution of dansyl chloride into a trichloromethane solution containing bromo-substituted alcohol and triethylamine in an inert atmosphere at room temperature, stirring and reacting at room temperature after the dropwise addition is finished, washing and drying a reaction solution after the reaction is finished, evaporating to remove a solvent, separating and purifying a reactant through column chromatography, and thus obtaining the dansyl derivative fluorescent probe. The fluorescence probe and methyl green-pyronin Y are used as a double-fluorescence probe, and a supermolecular proportional fluorescence sensing platform is constructed by utilizing the solubilizationeffect of a surfactant CTAB and is used for selectively detecting H2S.
Owner:TAIYUAN NORMAL UNIV

Improved mycobacterium tuberculosis rapid anti-acid fluorescence staining reagent

The invention discloses an improved mycobacterium tuberculosis rapid anti-acid fluorescence staining reagent comprising two reagents: an A liquid, comprising pure water, NaCl or KCl, dimethyl sulfoxide, glycerol and auramine O; and a B liquid, comprising pure water, hydrochloric acid, ethanol, and methyl green or Evans blue. Through formula optimization, auramine O fluorescence staining method isimproved and optimized, and a double-reagent type mycobacterium tuberculosis rapid anti-acid fluorescence staining reagent is developed in the invention, by which staining is completed just through two steps, and staining time is reduced from 20 min, in conventional processes, to about 2 min. The staining reagent is more convenient and quick to apply clinically and greatly increases the detectionefficiency of the mycobacterium tuberculosis.
Owner:江苏诺鬲生物科技有限公司

Environment-friendly dye and preparation method thereof

The invention discloses environment-friendly dye and a preparation method thereof. The environment-friendly dye is prepared from, in percentage, 30-40% of carbonate, 10%-20% of potassium hydroxide, 7%-15% of sodium oxalate, 4%-8% of methyl green, 5%-10% of basic fuchsin, 2%-5% of alginic acid, 1%-4% of arrowroot powder, 2%-5% of houttuynia cordata, 1%-3% of ramulus et uncus uncariae, 2%-4% of glycolic acid, 3%-5% of a composite halogen-free flame retardant, an anti-ultraviolet agent and a waterproof agent. The compound halogen-free flame retardant, the anti-ultraviolet agent and the waterproofagent are added to the formula, so that fire resistance, the anti-ultraviolet property and the waterproof property of the environment-friendly dye can be effectively improved, and accordingly, marketcompetitiveness of the environment-friendly dye is expanded; meanwhile, the preparation method of the dye is simple, so that the production cost is reduced, and the environment-friendly dye is in line with own interests of enterprises.
Owner:管洪化

Basic dye

The invention discloses a basic dye, which is composed of the following components in parts by weight: 14-16 parts of lichen red, 23-26 parts of methyl green, 31-33 parts of basic fuchsin, citron 10-16 parts of sodium acid, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of arrowroot powder and Houttuynia cordata 9‑10 servings. The invention has the advantages that: the basic dye of the technical proposal has no peculiar smell naturally, fast coloring and not easy to fade.
Owner:启东学顺五金配件有限公司

Natural alkaline dye

The invention discloses a natural alkaline dye which consists of the following components in part by weight: 23-26 parts of acid fuchsin, 14-16 parts of methylene blue, 23-26 parts of methyl green, 10-16 parts of sodium citrate, 13-18 parts of iron ions, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed solution, 21-24 parts of arrowroot powder and 9-10 parts of herba houttuyniae. The natural alkaline dye disclosed by the invention has the advantages that the natural alkaline dye in the technical scheme is natural, odor-free, fast to dye and hard to fade.
Owner:王世榕

A kind of magnetic polymer nano adsorption material lm-mp and its preparation method and application

The invention provides a magnetic polymer nanometer adsorption material LM‑MP and its preparation method and application. The adsorption material can efficiently adsorb cationic dye pollutants, and the synthesis method of the adsorption material is simple and low in cost. LM-MP was characterized by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, thermogravimetry, Zeta potential and other methods, and it was confirmed that Fe was grafted into the linear maltodextrin-pyromellitic dianhydride crosslinked polymer. 3 o 4 Magnetic nanoparticles, and have good magnetic properties. The adsorption material has large specific surface area, abundant hydroxyl and carboxyl groups and excellent superparamagnetic properties, and can be used as an adsorption material for cationic dye pollutants in aqueous solution. Using methyl green, malachite green, methyl violet, and ethyl violet dyes as model molecules to study the adsorption performance, it shows that the material can efficiently adsorb cationic organic dyes, and can be recycled without reducing the adsorption efficiency. It has application prospects in the aspect of environmental pollutant adsorption materials.
Owner:SHANXI UNIV

Natural non-toxic dye

The invention discloses natural non-toxic dye, which is prepared from the following ingredients in parts by weight: 14 to 16 parts of hematoxylin, 23 to 26 parts of methyl green, 31 to 33 parts of basic fuchsin, 10 to 16 parts of sodium citrate, 13 to 18 parts of iron ion, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed liquid, 21 to 24 parts of arrowroot flour and 9 to 10 parts of herba houttuyniae. The natural non-toxic dye has the advantages that the natural non-toxic dye using the technical scheme is natural; no foreign smell exists; the coloring is fast; the color fastness is realized.
Owner:邱永兴

Alkaline battery coating

The invention discloses alkaline battery coating. The alkaline battery coating is prepared from the following components in parts by weight: 14 to 16 parts of erythrin, 131 to 133 parts of Congo red,123 to 126 parts of methyl green, 61 to 83 parts of basic fuchsin, 13 to 18 parts of iron ions, 2 to 5 parts of alginic acid, 17 to 20 parts of fish meal, 40 to 50 parts of seed liquid, 21 to 24 partsof radix puerariae powder and 9 to 10 parts of herba houttuyniae. The alkaline battery coating disclosed by the invention has the advantages that the alkaline battery coating is natural and has no undesirable odor, is easy to color and is not easy to fade.
Owner:南通惠能镀镍钢带有限公司

Novel low-particle dye

The invention discloses a novel low-particle dye, which is composed of the following components by weight: 14-14 parts of Sudan III, 31-33 parts of eosin, 23-26 parts of methyl green, alkali 31-33 parts of basic fuchsin, 13-18 parts of iron ions, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of kudzu vine powder and9 to 10 parts of cordate houttuynia. The novel low-particle dye has the advantages that the novel low-particle dye is natural, non-odor, fast in coloring, and not easy to fade.
Owner:邱永兴

Novel weak acid dye

The invention discloses a novel weak acid dye. The novel weak acid dye consists of the following components in parts by weight: 14-16 parts of methyl blue, 31-33 parts of gentian violet, and 23-26 parts of methyl green. 31-33 parts of basic fuchsin, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of arrowroot powder And Houttuynia cordata 9‑10 parts. The invention has the advantages that: the novel weak acid dye of the technical proposal is naturally odorless, fast in coloring and not easy to fade.
Owner:张亚娟

A kind of immobilized spore laccase and its preparation and application

The invention discloses immobilized spore laccase as well as a preparation method and an application thereof, and belongs to the immobilized technical field and the wastewater treatment field. The immobilized spore laccase is formed by immobilizing spore laccase produced by a bacillus bacterial strain on DEAE(diethyl-aminoethanol)-cellulose through an ionic bond and a hydrogen bond, and has the extensive pH adaptive capacity and the heat resistnace of the spore laccase; in a solution containing an organic solvent, metal ions or an inhibitor, the immobilized spore laccase has stability which is stronger than that of free spore laccase. The preparation method of the immobilized spore laccase mainly comprises four steps of spore preparation, carrier pre-treatment, adsorption and collection, is simple and convenient to operate, and high in immobilization rate. The immobilized spore laccase provided by the invention has very strong dye discoloring capacity and has a highest discoloring rate up to about 95% while being applied to spinning dyes of methyl green, methyl red, cid red 1 and crystal violet. The carrier also can selectively adsorb the dyes to form complementary action with laccase.
Owner:JIANGNAN UNIV

Novel alkaline dye

The invention discloses a novel basic dye, which is composed of the following components in parts by weight: 14-16 parts of lichen red, 31-33 parts of Congo red, 23-26 parts of methyl green, 31-33 parts of basic fuchsin, 13-18 parts of iron ion, 2-5 parts of alginic acid, 10-12 parts of bean sprouts powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of arrowroot powder and fish 9-10 parts of Xingxue. The invention has the advantages that: the novel basic dye of the technical proposal is naturally odorless, fast in coloring and not easy to fade.
Owner:张亚娟

Non-toxic dye

The invention discloses non-toxic dye, which is prepared from the following ingredients in parts by weight: 14 to 16 parts of hematoxylin, 23 to 26 parts of methyl green, 31 to 33 parts of basic fuchsin, 10 to 16 parts of sodium citrate, 13 to 18 parts of iron ions, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed liquid, 21 to24 parts of arrowroot flour and 9 to 10 parts of herba houttuyniae. The non-toxic dye has the advantages that the non-toxic dye using the technical scheme is natural; no foreign smell exists; the coloring is fast; the color fastness is realized.
Owner:邱永兴

Coating with frosting effect

InactiveCN108822648APersonalizedWith hand feelCoatingsPersonalizationSodium stearate
The invention provides a coating with a frosting effect and relates to the technical field of building coatings. The coating comprises the following composition materials in parts by weight: 30-40 parts of pure acrylic elastic emulsion, 15-23 parts of polyacrylic emulsion, 7-15 parts of nano zinc oxide, 8-16 parts of sodium stearate, 2-8 parts of carbon black, 13-19 parts of rosin, 14-19 parts ofparaffin, 5-15 parts of asphalt, 9-17 parts of sebacic acid dihydrazide, 11-19 parts of sodium silicate, 11-19 parts of sodium silicate, 3-9 parts of methyl orange, 5-12 parts of methyl green, 15-20 parts of calcium chloride, 8-15 parts of butyl acetate, 4-14 parts of ethyl cellulose, 5-12 parts of a filler, and 4 parts of sand. The coating provided by the invention has the advantages of having certain personalization and hand feeling.
Owner:佛山市高明区生产力促进中心

Composite alkaline battery coating

The invention discloses composite alkaline battery coating. The composite alkaline battery coating is prepared from the following components in parts by weight: 14-16 parts of orcein, 131-133 parts ofcongo red, 23-26 parts of methyl green, 61-83 parts of basic fuchsin, 13-18 parts of iron ions, 2-5 parts of alginic acid, 17-20 parts of fish meal, 40-50 parts of seed solution, 21-24 parts of arrowroot powder and 9-10 parts of herba houttuyniae. The composite alkaline battery coating has the advantages that the composite alkaline battery coating provided by the technical scheme is nature, freeof undesirable smell, rapid to color and not prone to fading.
Owner:南通惠能镀镍钢带有限公司

A method and device for detecting sulfide

The invention relates to a sulfide detection method and device, which is to acidify the sulfide, convert the sulfide into hydrogen sulfide and separate it from the complex matrix, and use the inert gas nitrogen as the carrier gas to carry the generated hydrogen sulfide out into the The gas channel, when encountering the filter paper placed in the gas channel and adsorbed with methyl green, a reagent that has a selective response to hydrogen sulfide, the released hydrogen sulfide gas is enriched on the filter paper and reacts with methyl green. The color of methyl green changes from dark green to light green or colorless, and the intensity of light signal is recorded in real time, which simplifies the operation process of determining sulfide and reduces the interference caused by the complex matrix where endogenous sulfide is located. The invention enriches the hydrogen sulfide gas on the filter paper, reduces the detection limit, improves the detection sensitivity, and relatively low operating cost.
Owner:SHAANXI NORMAL UNIV

Method for identifying purity of corn seeds

The invention discloses a method for identifying the purity of corn seeds. Compared with the prior art, by adjusting the concentrations of acrylamide and N,N-methylene bisacrylamide (MBA) in the method, for the method, the toughness and the rigidity of separation gel sheets are greatly improved; stacking gel is omitted, electrophoretic velocity is slowed down by reducing the voltage, and proteins with different molecular weights can be gathered on the same horizontal line before entering separation gel; methyl green AR-0721 is screened out in the method, and the migration rate of the methyl green AR-0721 is synchronous with the migration rate of protein molecules, thus being convenient to check whether the test procedure is finished; the sample extracting temperature and dyeing conditions are improved, the extraction and identification time is greatly shortened, the identification efficiency is improved, and the fixed procedure of the separation gel is simplified at the same time; the cleaning and storing procedures of the separation gel sheets are added, a cleaning solution and a storage solution are developed, and both the test result identification and the separation gel sheet storage are convenient.
Owner:SHANDONG CROP GERMPLASM CENT

Natural weak-acidic dye

The invention discloses a natural weak-acidic dye composed of, by weight, 14-16 parts of neutral red, 23-26 parts of methyl green, 31-33 parts of basic fuchsin, 10-16 parts of sodium citrate, 13-18 parts of iron ions, 2-5 parts of alginic acid, 10-12 parts of bean sprout powder, 17-20 parts of fish meal, 11-18 parts of seed liquid, 21-24 parts of kudzu powder, and 9-10 parts of houttuynia cordata.The dye is natural and odor-free, can quickly colorize an object and is not liable to cause discoloration.
Owner:王世榕

Weak acid dye

The invention discloses weak acid dye, which is prepared from the following ingredients in parts by weight: 14 to 16 parts of methyl blue, 23 to 26 parts of methyl green, 31 to 33 parts of basic fuchsin, 10 to 16 parts of sodium citrate, 13 to 18 parts of iron ions, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed liquid, 21 to24 parts of arrowroot flour and 9 to10 parts of herba houttuyniae. The weak acid dye has the advantages that the weak acid dye adopting the technical scheme is natural and does not have foreign odor;the coloring speed is high; the color fading cannot easily occur.
Owner:邱永兴

Novel weak acidic dyestuff

The invention discloses a novel weak acidic dyestuff. The novel weak acidic dyestuff is prepared from the following components in parts by weight: 14 to 16 parts of methyl blue, 31 to 33 parts of gentian violet, 23 to 26 parts of methyl green, 31 to 33 parts of basic fuchsin, 13 to 18 parts of iron ions, 2 to 5 parts of alginic acid, 10 to 12 parts of bean sprout powder, 17 to 20 parts of fish meal, 11 to 18 parts of seed solution, 21 to 24 parts of radix puerariae powder and 9 to 10 parts of herba houttuyniae. The novel weak acidic dyestuff disclosed by the invention is natural and has the advantages of no undesirable odor, rapidness in coloring and uneasiness of fading.
Owner:郁锡群
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