Method for identifying purity of corn seeds

A purity identification and seed technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of insufficient film toughness and hardness, long time-consuming protein extraction, and cumbersome gel-making procedures. The effect of easy test operation and observation, and simple process flow

Inactive Publication Date: 2015-01-07
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional electrophoresis technology has the following problems in the operation process: time-consuming protein extraction, insufficient toughness and hardness of the film, cumbersome gel preparation procedures, and low dyeing efficiency, which reduce the efficiency of the test.

Method used

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  • Method for identifying purity of corn seeds
  • Method for identifying purity of corn seeds

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Experimental program
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Effect test

Embodiment 1

[0037] 1. Solution preparation

[0038] (1) Electrode buffer

[0039] Weigh 6.00g of glycine, pour it into a 2000mL beaker, add 1800mL of deionized water to dissolve, adjust the pH to 3.3 with 2.0ml of lactic acid, add deionized water to 2000mL, and mix well.

[0040] (2) Sample extract

[0041] Weigh 5.80g of sodium chloride, 200.00g of sucrose, and 0.1515g of methyl green AR-0721 (molecular weight: 608.75), pour them into a 1000mL beaker, and add 800ml of deionized water. Dissolve, heat to a slight boil, let it cool to room temperature, and then dilute to 1000mL with deionized water. Store at 4°C.

[0042] (3) Separating gel solution

[0043] Take 1.43mL of sodium lactate and add 980mL of deionized water, then add 129.38g of acrylic acid amine, 4.31g of N,N'-methylenebisacrylamide, 0.25g of ascorbic acid, and 8.0mg of ferrous sulfate. After dissolving, adjust the pH to 3. 0, add deionized water to make up to 1000mL, filter into a brown bottle, and store at 4°C.

[0044...

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Abstract

The invention discloses a method for identifying the purity of corn seeds. Compared with the prior art, by adjusting the concentrations of acrylamide and N,N-methylene bisacrylamide (MBA) in the method, for the method, the toughness and the rigidity of separation gel sheets are greatly improved; stacking gel is omitted, electrophoretic velocity is slowed down by reducing the voltage, and proteins with different molecular weights can be gathered on the same horizontal line before entering separation gel; methyl green AR-0721 is screened out in the method, and the migration rate of the methyl green AR-0721 is synchronous with the migration rate of protein molecules, thus being convenient to check whether the test procedure is finished; the sample extracting temperature and dyeing conditions are improved, the extraction and identification time is greatly shortened, the identification efficiency is improved, and the fixed procedure of the separation gel is simplified at the same time; the cleaning and storing procedures of the separation gel sheets are added, a cleaning solution and a storage solution are developed, and both the test result identification and the separation gel sheet storage are convenient.

Description

technical field [0001] The invention relates to a method for identifying the purity of corn seeds, which is specifically applied to the fields of quick identification of seed purity and identification of germplasm resources. Background technique [0002] Variety purity is the main quality index of corn seeds, which has a direct and significant impact on the quality of corn and product quality. The loss of maize production in my country due to low seed purity is staggering, and the reduction in yield due to low purity alone may offset the potential for a new variety to increase yield. [0003] Maize seed purity identification technology is one of the key points and difficulties in my country's current seed inspection work, and it is also an urgent problem to be solved in seed management work. At present, the methods for identifying the purity of seeds mainly include: morphological identification, protein electrophoresis, and molecular marker identification based on DNA molec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447G01N1/28
Inventor 颜廷进李群王效睦孔维国田茜戴双张文兰段乃彬
Owner SHANDONG CROP GERMPLASM CENT
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