A kind of immobilized spore laccase and its preparation and application

A spore laccase and spore technology, applied in the field of immobilized spore laccase and its preparation and application, can solve the problems of difficult porous material adsorption, inextensibility, heavy weight, etc., achieve strong decolorization ability, strong stability, and easy operation Effect
CN104745567BActive Publication Date: 2018-08-24JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2018-08-24

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Abstract

The invention discloses immobilized spore laccase as well as a preparation method and an application thereof, and belongs to the immobilized technical field and the wastewater treatment field. The immobilized spore laccase is formed by immobilizing spore laccase produced by a bacillus bacterial strain on DEAE(diethyl-aminoethanol)-cellulose through an ionic bond and a hydrogen bond, and has the extensive pH adaptive capacity and the heat resistnace of the spore laccase; in a solution containing an organic solvent, metal ions or an inhibitor, the immobilized spore laccase has stability which is stronger than that of free spore laccase. The preparation method of the immobilized spore laccase mainly comprises four steps of spore preparation, carrier pre-treatment, adsorption and collection, is simple and convenient to operate, and high in immobilization rate. The immobilized spore laccase provided by the invention has very strong dye discoloring capacity and has a highest discoloring rate up to about 95% while being applied to spinning dyes of methyl green, methyl red, cid red 1 and crystal violet. The carrier also can selectively adsorb the dyes to form complementary action with laccase.
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Description

technical field

[0001] The invention relates to an immobilized spore laccase and its preparation and application, belonging to the technical field of immobilization and the field of wastewater treatment. Background technique

[0002] Laccase is a copper-containing polyphenol oxidase widely found in fungi, bacteria, plants and insects. Laccase can oxidize many phenolic and nonphenolic substances, including monophenols, diphenols, polyphenols, aminophenols, methoxyphenols, aromatic amines, and ascorbates. Laccase can reduce oxygen to water while oxidizing the substrate, and it will not produce harmful substances, so it is a very environmentally friendly biocatalyst. Laccase has good application prospects in paper industry, wastewater treatment, textile industry, food processing, biosensor and biodiagnosis due to its broad substrate spectrum and strong oxidizing ability.

[0003] In recent years, scholars have discovered that bacterial laccases are extremely stable in alkalin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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