The invention discloses a method for extracting
adenosine, comprising the following steps of: A, adjusting pH value of an
adenosine fermentation broth to 7-8 by the use of aqueous alkali, heating, stirring, adding a flocculating agent, adjusting the rotating speed to 60+ / -10 r / m after 60+ / -10 minutes, stirring for 5-7 minutes, standing for 10-15 minutes, followed by press
filtration; B, condensing the filtrate obtained from the step A in a rotary film
evaporator to obtain a concentrate while the
condensation temperature is controlled within 70+ / -10 DEG C and degree of vacuum is -0.095+ / -0.01 Mpa; C, placing the concentrate obtained form the step B at
room temperature, naturally cooling for 2+ / -1 h, continuously cooling to below zero, followed by freezing
crystallization to obtain a crystalline liquid; and D, carrying out pumping
filtration on the frozen crystalline liquid obtained from the step C to obtain a wet product, putting the wet product into a baking oven, and
drying for 10+ / -1 h so as to obtain a dry
adenosine crude product. By the adoption of the method for extracting adenosine from the
fermentation broth, the obtained adenosine crude product contains little content of impurities, the loss of the product adenosine is little, and the adenosine yield of a subsequent purification technology and a refining technology is obviously raised. In addition, the whole extraction method is economical and effective.