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Acid-producing klebsiella oxytoca MOW-02-05, selection method and application of acid-producing klebsiella oxytoca MOW-02-05

A technology of Klebsiella and strains, applied in the field of Klebsiella oxytoca MOW-02-05 and its screening, can solve the problems of difficult biodegradation and purification treatment of dye wastewater, and achieve a wide range of practical application prospects , the effect of good decolorization ability

Inactive Publication Date: 2013-04-17
SUZHOU INST FOR ADVANCED STUDY USTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Klebsiella oxytoca (Klebsiellaoxytoca) MOW-02-05, which solves the problems in the prior art that the dye wastewater is difficult to be biodegraded and purified.

Method used

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  • Acid-producing klebsiella oxytoca MOW-02-05, selection method and application of acid-producing klebsiella oxytoca MOW-02-05
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  • Acid-producing klebsiella oxytoca MOW-02-05, selection method and application of acid-producing klebsiella oxytoca MOW-02-05

Examples

Experimental program
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Effect test

Embodiment 1

[0063] The screening of embodiment 1 thalline

[0064] 1) When the intermittent anaerobic sludge reactor stops intermittently, take 1ml of the bottom sludge from the reactor; 2) Add it to a serum bottle filled with nitrogen and filled with 100ml of screening medium, set the temperature at 35°C and the speed at Shake on a 120rpm shaker until the liquid in the serum bottle is colorless; 3) Take 1ml of the bacterial solution in the serum bottle at this time, and repeat step 2 to obtain the bacterial solution after screening 5 times; 4) Take 1ml of the bacterial solution that has been screened 5 times solution, diluted 5,000 times with sterile water, and spread on a solid LB medium plate, and obtained a single strain by streaking the plate; 5) Pick a single strain on the plate medium with an inoculation loop, and insert it into LB Grow aerobically in the liquid medium for 8 hours, centrifuge, and collect the bacteria; 6) Collect the collected bacteria, repeat step 2, and use visua...

Embodiment 2

[0067] The cultivation of embodiment 2 thalline

[0068]Use an inoculation loop to pick up the bacterial strain Klebsiella oxytocaMOW-02-05 on the activated plate medium and transfer it to the Erlenmeyer flask containing the strain activation medium, and shake it for 8 hours in a shaker with a set temperature of 35°C and a rotational speed of 180rpm , the obtained cells were centrifuged (8,000g) for 20 minutes, the cells were washed with phosphate buffered saline, and centrifuged again, repeated 3 times to obtain milky white pollution-free strain cells.

[0069] As mentioned above, the composition of the medium for cultivating the strain is: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, adding water to 1 L, pH7.

Embodiment 3

[0070] Embodiment 3 thalline morphology observation

[0071] Light Microscopic Observation of Bacteria

[0072] The bacteria were collected according to the method of Example 2, and the bacteria were fixed by a conventional flame fixation method, and then placed under an optical microscope for staining experiment observation.

[0073] Scanning Electron Microscopy (SEM) Observation of Bacteria

[0074] Collect the thalli according to the method of Example 2, wash three times with 50mM phosphate buffered saline (PBS), then fix overnight with 2.5% glutaraldehyde, then wash three times with PBS, finally dehydrate with ethanol successively, vacuum-dry to make sample, Observed by SEM after spraying gold.

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Abstract

The invention discloses an acid-producing klebsiella oxytoca MOW-02-05, a selection method and an application of the acid-producing Klebsiella oxytoca MOW-02-05, wherein the acid-producing klebsiella oxytoca MOW-02-05 is isolated from an intermittent anaerobic sludge reactor and can efficiently degrade dyes and organisms to produce hydrogen; and the klebsiella oxytoca MOW-02-05 is used for decolorizing azo dyes and biologically produce the hydrogen from organic carbon resources. The strain is identified as klebsiella oxytoca MOW-02-05 and is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5237. The strain obtained by the invention can solve a problem caused by acidifying the reactor when a conventional anaerobic reactor treats azo dye wastewater, and is suitable for biological treatment of printing and dyeing dye wastewater.

Description

technical field [0001] The invention belongs to the technical field of purification and treatment of dye wastewater, and in particular relates to Klebsiella oxytoca MOW-02-05 and its screening method and application. Background technique [0002] Azo dyes are the most important species of synthetic dyes, widely used in printing and dyeing, textile and papermaking industries. Azo dyes have a -N=N- chromophoric group, which requires electrons to decolorize and reduce the azo bond. However, due to the presence of oxygen in the air, they are stable and difficult to be degraded by microorganisms in the natural environment. On the one hand, the discharge of azo dyes will increase the chroma of the water body, inhibit the process of photosynthesis in the water body, and then affect the growth of organisms at all levels of the biological chain, destroying the regional water ecosystem; on the other hand, the intermediate products of dye degradation ( Mostly aromatic amine compounds)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12P3/00C12R1/22C02F101/38
Inventor 虞磊俞汉青李文卫
Owner SUZHOU INST FOR ADVANCED STUDY USTC
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