Saccharomycete and its application

A technology of yeast and strains, which is applied in the field of environmental biology, can solve the problems of lack of yeast strains and limited application, and achieve the effect of simple cultivation method, fast growth speed, and not easy to mutate

Inactive Publication Date: 2007-10-24
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its application in the treatment of dye wastewater is still very limited, one of the important reasons is the lack of yeast strains that can effectively decolorize dyes

Method used

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  • Saccharomycete and its application
  • Saccharomycete and its application

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1, the cultivation of Pseudozyma rugulosa Y-63 CGMCC No.1247 thalline

[0018] Use an inoculation loop to pick the yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1247 and connect it to the slant (glucose 2%, peptone 2%, yeast powder 1%, pH natural, agar 2%), and set the temperature at 28 ° C for cultivation In the box, cultivate for 1-3 days, and white bacteria appear. Then, use the inoculation loop to pick the well-grown thalline from the slope and connect it to a triangular flask equipped with culture solution (2% glucose, 2% peptone, 1% yeast powder, natural pH), set the temperature at 28°C, Cultivate for 1-3 days on a shaking table with a rotating speed of 200rpm, centrifuge the obtained cells (9000rpm) for 10min, wash the cells with sterile normal saline (0.8%) and sterile water, then centrifuge, and repeat 2-3 times to obtain White uncontaminated yeast cells of Pseudozyma rugulosa Y-63 CGMCC No.1247.

[0019] The above-mentioned culture medium and cul...

Embodiment 2

[0020] Embodiment 2, Pseudozyma rugulosa Y-63 CGMCC No.1247 to the decolorization of reactive brilliant red K-2BP and reactive black KN-B

[0021] Reactive Brilliant Red K-2BP Decolorization Medium: Glucose 1%, KH 2 PO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.05%, yeast powder 0.02%, reactive brilliant red K-2BP 200mg / L 50mg / L, natural pH.

[0022] Depigmentation Medium for Active Black KN-B: Glucose 1%, KH 2 PO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.05%, yeast powder 0.02%, active black KN-B 50mg / L, pH natural.

[0023] According to the method of embodiment 1, with liquid culture medium (glucose 1%, KH 2 PO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.05%, yeast powder 0.02%, pH natural) yeast strain Pseudozyma rugulosa Y-63CGMCC No.1247 was cultivated to the logarithmic phase, and transferred to reactive brilliant red K-2BP and In the decolorization medium of Active Black KN-B, cultivate on a shaker, fill each 50ml Erlenmeyer flask with 20ml of decolorization l...

Embodiment 3

[0031] Embodiment 3, Pseudozyma rugulosa Y-63 CGMCC No.1247 to the decolorization of medium bleaching blue B

[0032] According to the method of Example 2, the yeast strain Pseudozyma rugulosa Y-63 CGMCC No.1247 was decolorized in the medium bleaching blue B decolorization medium (glucose 1%, KH 2 PO 4 0.1%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.05%, yeast powder 0.02%, medium Bleach Bleu B (formula III) 50mg / L, pH natural) and cultured for 24h, the experimental results showed that the decolorization rate of the bacterial strain to 50mg / L medium Bleach Bleu B was 91%.

[0033] (Formula III)

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Abstract

The present invention discloses one kind of saccharomycetes and its application, and provides one saccharomycete strain, Pseudozyma rugulosa Y-63 CGMCC No. 1247, with high dye decolorizing ability and its usage in decolorizing dye. The saccharomycete strain, Pseudozyma rugulosa Y-63 CGMCC No. 1247, has very high activity, very powerful dye decolorizing ability, simple culture process, fast growth speed and less mutation, and may be used as the template strain for the research of dye decolorizing mechanism. What is more important is that the saccharomycete strain may be used directly in decolorizing dye and has industrial application foreground in dye waste water treatment.

Description

technical field [0001] The invention relates to a strain of yeast and its application in the field of environmental biotechnology. Background technique [0002] Dyes used in the textile industry usually have complex synthetic domains and aromatic ring structures, making them very stable in the environment and not easy to degrade. They are often discharged into the environment along with the dye wastewater, causing serious pollution to the environment. In order to reduce the harm of such substances, in addition to conventional physical and chemical methods, it is more and more attractive to adopt new and environmentally friendly biotechnology treatment methods. Yeast is a single-cell fungal microorganism. It not only has the characteristics of fast growth and easy operation of single-cell bacteria, but also can resist adverse growth environments like filamentous fungi. Therefore, it is suitable for environmental governance. At present, its application in the environmental f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C02F3/34
Inventor 文湘华余志晟
Owner TSINGHUA UNIV
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