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Bacterial laccase gene and expression and application thereof

A technology of bacteria and laccase, applied in the direction of application, bacteria, genetic engineering, etc., can solve the problems of no more than 20, no bacterial laccase, etc., and achieve good stability and strong decolorization effect

Active Publication Date: 2011-01-26
ANHUI UNIVERSITY
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AI Technical Summary

Problems solved by technology

At present, laccases have been found in microorganisms such as Bacillus licheniformis and Bacillus subtilis, but there are no more than 20 bacterial laccase genes that have been systematically studied, which need further development and research, and there is currently no Report on the Industrial Application of Bacterial Laccase

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  • Bacterial laccase gene and expression and application thereof
  • Bacterial laccase gene and expression and application thereof
  • Bacterial laccase gene and expression and application thereof

Examples

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Embodiment Construction

[0034] The implementation methods in the following examples are conventional methods unless otherwise specified.

[0035] (1), the construction of the expression bacterial strain that contains bacterial laccase gene of the present invention

[0036] 1. Screening of positive clones containing bacterial laccase gene of the present invention and acquisition of full-length gene

[0037] From 100L (24°21′17N, 118°08′59E) of ocean surface seawater in the South China Sea, isolate microorganisms and extract genome, the separation and extraction method is the same as the application number CN200810024342.1, provided according to the kit (purchased from Epicentre company) Instructions for constructing a metagenomic library. (Chu XM, He HZ, Guo CQ, Sun BL, 2008. Identification of two novel esterases from a marine metagenomic library derived from South China Sea. Appli. Microbiol. Biotechnol. 80, 615-625.) The obtained single clones were placed in 384-well plates and stored at -70°C.

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Abstract

The invention discloses a bacterial laccase gene and expression and application thereof. The bacterial laccase gene has a gene code sequence shown as SEQ ID No:1 and is polypeptide with an amino acid sequence shown as SEQ ID No:2. The invention also discloses an expression strain with the bacterial laccase. The bacterial laccase is taken from uncultured marine microorganisms and has the advantages of high stability in the pH range of 6 to 8, half-life period of 73 minutes at the temperature of 45 DEG C, high decoloration for an azo dye, and capacity of decolorizing industrial dyes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacterial laccase and its expression, as well as the industrial application of the bacterial laccase protein. Background technique [0002] Laccase (Laccase, EC 1.10.3.2) is a class of copper-containing polyphenol oxidases that catalyze the oxidation of a variety of phenolic and nonphenolic compounds accompanied by the reduction of molecular oxygen to water. Laccase has a wide range of substrates and high catalytic efficiency. It is widely used in the fields of biopulping and bleaching, food flavor improvement, feed nutrition improvement, artificial dye decolorization, textile fiber softening and refinement, new drug development, biosensor manufacturing and new energy development. It has potential important application value, and has become a research hotspot in the interdisciplinary field of international enzyme engineering and environmental science in recent years. [0003] Lacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/63C12N1/15C12N1/19C12N1/21C12N15/66C02F3/00C12R1/19C02F103/30
Inventor 肖亚中方泽民李同亮张学成彭惠房伟洪宇植
Owner ANHUI UNIVERSITY
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