Acinetobacter baumannii, and screening method and application thereof in degradation of azo dye Congo red

A technology of Acinetobacter baumannii and azo dyes, applied in the field of environmental engineering and bioengineering, can solve the problems of large differences in the structure of azo reductase, the inability of general microorganisms, and the difficulty of growth and reproduction, etc., to enrich the decolorization mechanism of dyes , good tolerance and removal effect, not easy to change the effect

Active Publication Date: 2014-04-30
广州市泓绿环保科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of practices in research and engineering applications have shown that the particularity of dye wastewater makes it impossible or difficult for general microorganisms to grow, reproduce and perform degradation in such wastewater
At the same time, the currently discovered azo dye-degrading bacteria are generally anaerobic degrading bacteria, and the experimental research results of domestic and foreign scholars show that anaerobic microorganisms have certain decolorization and degradation effects on dye wastewater through reduction, but the treated water contains a large amount of Highly toxic and highly carcinogenic intermediate products, such as aromatic amine compounds, etc.
For a single aerobic degradation, there are few reports at present, and it can be seen from the reported literature that the azoreductases produced by them are quite different in structure
[0004] At the same time, there is no literature report on the use of Acinetobacter baumannii to degrade dyes

Method used

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  • Acinetobacter baumannii, and screening method and application thereof in degradation of azo dye Congo red
  • Acinetobacter baumannii, and screening method and application thereof in degradation of azo dye Congo red
  • Acinetobacter baumannii, and screening method and application thereof in degradation of azo dye Congo red

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Acinetobacter baumannii ( Acinetobacter baumannii ) Screening of YNWH 226

[0034] S1. Take 10mL of wastewater from the aerobic tank of a printing and dyeing wastewater treatment plant in Dongguan City in 100mL of LB medium, and cultivate it at 30°C and 150rpm for 16 hours to obtain the initial culture solution;

[0035] S2. Take out 10 mL of the primary culture solution described in S1 and put it in 100 mL of inorganic salt medium containing 1 g / L Congo red, and cultivate it for two days at 30° C. and 150 rpm to obtain the culture solution;

[0036] S3. Take out 10 mL of the culture solution described in S2 and put it in a fresh inorganic salt medium containing 1 g / L Congo red, cultivate it for two days at 30° C. at 150 rpm, and repeat S63 3 times to obtain the final culture solution;

[0037] S4. Take 10 mL of the final culture solution described in S3 and properly dilute it, then evenly spread it on a solid inorganic salt medium plate containing 1 g / L...

Embodiment 2

[0044] Embodiment 2 Acinetobacter baumannii ( Acinetobacter baumannii ) Application of YNWH 226 in degrading Congo red

[0045] 1. The strain YNWH 226 was cultured in LB medium for 16 hours (late logarithmic growth stage), and 5 mL was inoculated until the concentrations of the azo dye Congo red were 0.1 g / L, 0.2 g / L, 0.3 g / L, and 0.4 g / L, respectively. L, 0.5g / L 200mL inorganic salt medium, cultured at 30°C, 150rpm, pH7.0 for 5 days. During this period, 5 mL of the culture solution was taken every 24 hours, centrifuged for 2 minutes under the action of 700 g centrifugal force, and the supernatant was taken.

[0046]2. Measure the absorbance of the supernatant obtained in step 1 at 490nm using a UV-Vis spectrophotometer (Puyi TU-1901), and calculate the degradation rate of the dye with the dye culture solution without adding bacteria as a control.

[0047] The results are attached figure 1 Shown: the degradation rate of strain YNWH 226 to 100mg / L Congo red is 90.37%, t...

Embodiment 3

[0049] Embodiment 3 Acinetobacter baumannii ( Acinetobacter baumannii ) Application of YNWH 226 in degrading Congo red

[0050] Cultivate strain YNWH 226 in LB medium for 16 hours (late logarithmic growth period), take 10 mL of ice bath for 30 min, centrifuge at 4°C and 4000 rpm for 5 minutes, discard the supernatant, and wash the bacteria with sterile water Twice, and then inoculated into 200mL inorganic salt medium containing the azo dye Congo red at a concentration of 0.1g / L, and cultured for 5 days according to the conditions in Table 1. During this period, 5 mL of culture solution was taken every 12 hours, centrifuged for 2 minutes under the action of 700 g centrifugal force, and the supernatant was taken. The obtained supernatant was measured at 490nm by a UV-Vis spectrophotometer (Puyi TU-1901), and the degradation rate of the dye was calculated using the culture solution without bacteria and dye as a control. The result is as figure 2 shown. The statistical resu...

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Abstract

The invention discloses acinetobacter baumannii, and a screening method and an application thereof in degradation of an azo dye Congo red. The stain is named as acinetobacter baumannii YNWH226, is preserved in China general microbiological culture collection center, and has the preservation number of CGMCC No.7922. The invention provides the new acinetobacter baumannii and the application thereof in degradation of the dye. Especially, the acinetobacter baumannii can effectively degrade the azo dye Congo red, has quite good decoloration and mineralization effects on Congo red wastewater, can completely mineralize the Congo red under a simple aerobic condition, and has great significance on governance of dye pollution and study on aerobic degradation of the azo dye.

Description

technical field [0001] The invention relates to the technical fields of biological engineering and environmental engineering. More specifically, it relates to a strain of Acinetobacter baumannii, its screening method and its application in degrading the azo dye Congo red. Background technique [0002] Azo dyes are a class of aromatic compounds containing one or more -N=N- functional groups, accounting for about 80% of the total organic dye products. According to the dye chromophore, its chromophore is an azo double bond (-N=N-), and the auxochrome is an amino group, a hydroxyl group, a methyl group, and a sulfonic acid group. Produces various colors perceived by vision. Congo red is the first synthetic azo dye. It can be seen from its structural formula that Congo red is a reactive dye containing two azo double bonds. It has good water solubility, but there are a lot of dyes in the dyeing process. It is discharged in the form of waste water without being attached to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/38
CPCY02W10/10
Inventor 宁寻安阳重阳王玉洁梁昕王靖宇陈国鑫林美卿李锐敬陈泓刘敬勇杨佐毅
Owner 广州市泓绿环保科技有限公司
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