Method for extracting adenosine

Abstract

The invention discloses a method for extracting adenosine, comprising the following steps of: A, adjusting pH value of an adenosine fermentation broth to 7-8 by the use of aqueous alkali, heating, stirring, adding a flocculating agent, adjusting the rotating speed to 60+ / -10 r / m after 60+ / -10 minutes, stirring for 5-7 minutes, standing for 10-15 minutes, followed by press filtration; B, condensing the filtrate obtained from the step A in a rotary film evaporator to obtain a concentrate while the condensation temperature is controlled within 70+ / -10 DEG C and degree of vacuum is -0.095+ / -0.01 Mpa; C, placing the concentrate obtained form the step B at room temperature, naturally cooling for 2+ / -1 h, continuously cooling to below zero, followed by freezing crystallization to obtain a crystalline liquid; and D, carrying out pumping filtration on the frozen crystalline liquid obtained from the step C to obtain a wet product, putting the wet product into a baking oven, and drying for 10+ / -1 h so as to obtain a dry adenosine crude product. By the adoption of the method for extracting adenosine from the fermentation broth, the obtained adenosine crude product contains little content of impurities, the loss of the product adenosine is little, and the adenosine yield of a subsequent purification technology and a refining technology is obviously raised. In addition, the whole extraction method is economical and effective.

Description

technical field [0001] The invention relates to a method for separating and extracting crude adenosine from fermentation broth Background technique [0002] As a drug, adenosine was first put into the market internationally by Lyhomed, a subsidiary of Japan's Fujisawa Pharmaceutical Factory, in the United States in 1989, and then in the United Kingdom. Belgium. France and many other countries have been approved, adenosine, also known as adenosine, molecular formula C 10 h 13 N 5 o 4 , molecular weight 267.24, odorless, tasteless, white crystalline powder, hardly soluble in water, ethanol and ether, melting point 233-238°C, specific rotation -68.0°~-72.0° is an endogenous nucleus that spreads all over human cells Glycosides can directly enter the myocardium to be phosphorylated to generate adenosine, participate in myocardial energy metabolism, and also participate in dilating coronary vessels and increasing blood flow. Adenosine has physiological effects on the cardiov...

AI Technical Summary

Problems solved by technology

Since biological fermentation is also a complex system, at the same time, microbial cells and debris cells, sugar and protein sticky substances increase the viscosity of the fermentation liquid, making it difficult to remove solid impurities.

At present, the solid-liquid separation methods of fermentation broth mainly adopt the following methods: high-speed centrifugation, which requires a large investment in equipment and high operation and maintenance costs; ultrafiltration, which is simple to operate and has a high product yield, but the ultrafiltration membrane Difficult to maintain, short practical cycle; coagulation method (adding aluminum sulfate and other aluminum salt coagulant alone), strong stirring or centrifugation for solid-liquid separation, because there are a large number of colloidal impurities with different molecules in the fermentation broth, and aluminum The salt molecular weight is small, the charge is low, it is difficult to completely flocculate and precipitate, so the separation and removal rate is low, and the product yield is not high, especially for adenosine fermentation broth, it is difficult to completely flocculate and precipitate