Aerobic active marine bacteria and preparation method of decolorizing flocculant of aerobic active marine bacteria

A technology of decolorizing flocculant and marine bacteria, which is applied in the field of water purification to achieve high economic benefits, good decolorization effect and simple preparation steps

Active Publication Date: 2017-12-12
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General Microbiology Center (CGMCC), China Committee for Culture Collection of Microorganisms, is located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] An aerobically active marine bacterium was isolated and screened from the spitting mud of Philippine clams cultured in a marine farm in Zhujiajian, Zhoushan, Zhejiang, China, and was identified as Pseudoalteromonas ( Pseudoalteromonassp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), and the preservation number is CGMCC No.12913. The General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0020] The preparation method of decolorizing flocculant comprises the following steps:

[0021] 1) Seed liquid culture: inoculate the slant culture medium with marine bacteria with the preservation number CGMCC No. 12913, and culture at a constant temperature of 25°C for 36 hours. Add an equal volume of sterile water and shake to obtain a seed solution. The c...

Embodiment 2

[0027] The most preferred preparation method of decolorizing flocculant comprises the following steps:

[0028] 1) Seed liquid culture: inoculate the slant culture medium with marine bacteria with the preservation number CGMCC No. 12913, and culture at a constant temperature of 25°C for 36 hours. Add an equal volume of sterile water and shake to obtain a seed solution. The composition and concentration of the slant medium are glucose 22g / L, urea 0.47g / L, ammonium sulfate 0.2g / L, yeast extract 0.5g / L, dipotassium hydrogen phosphate 2.7g / L, potassium dihydrogen phosphate 1.1g / L , agar 13g / L and the remainder aged seawater or artificial seawater; adjust the pH to 7.3, and autoclave at 121°C for 25min. Among them, the artificial seawater formula is: sodium chloride 25.0 g / L, magnesium chloride 1.9 g / L, magnesium sulfate 3.1 g / L, calcium chloride 1.3 g / L, sodium bicarbonate 0.2 g / L, potassium chloride 0.68 g / L L, sodium bromide 0.06 g / L, boric acid 0.058 g / L, sodium silicate 0.00...

Embodiment 3

[0032] Example 3: Determination of flocculation activity

[0033] Adopt flocculation activity test method (R.Kurane, K.Takeda, T.Suzuki.Screening and Characteristics of Microbial Flocculants.Agric.Biol.Chem.1986: 2301-2307) to measure the flocculation activity of kaolin suspension, take 5g / L of kaolin suspension 93ml, add 5ml 1% (m / v) CaCl 2 As a coagulation aid, add 2ml of the sample of Example 2, adjust the pH value to 9, mix and stir quickly for 1min, then stir slowly for 5min, let stand for 10min, take the mixed solution at 1cm below the water level, and measure the absorbance A at 550nm with a visible spectrophotometer , while using 2ml of distilled water instead of the sample as a control, measure the absorbance B, and calculate the flocculation rate, FR(%) = (A-B) / A×100%. The flocculation rate of marine bacterial decolorization flocculant to kaolin suspension is 91.41%.

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Abstract

The invention discloses aerobic active marine bacteria and a preparation method of a decolorizing flocculant of the aerobic active marine bacteria. The aerobic active marine bacteria is formed by performing isolation and screening on mud disgorged by ruditapes philippinarum bred in a sea farm in a Zhujiajian, Zhoushan, Zhejiang in China, and is authenticated to be pseudoalteromonas ((i) Pseudoalteromonas (/i)sp.). The decolorizing flocculant has the beneficial effects that the formed adecolorizing flocculant has good decoloration effect, and the flocculation rate of the decolorizing flocculant to kaolin reaches greater than 90 percent; the decolorizing flocculant has high activity for performing decoloration on high-concentration waste water, and the flocculation rate of the decolorizing flocculant to methylene blue, malachite green and crystal violet reaches greater than 92 percent, 99 percent and 98 percent; the decolorizing flocculant does not have potential toxicity for organisms, and is green and healthy.

Description

technical field [0001] The invention relates to the technical field of water purification, in particular to a preparation method of an aerobic active marine bacterium and a decolorizing flocculant thereof. Background technique [0002] In the treatment of industrial water, drinking water and domestic sewage, the performance of flocculants is very important. In the flocculation process of water treatment, the nano-scale, micron-scale suspension or colloidal impurity particles in the water are mainly formed into large floc particles through the coagulation-flocculation reaction under the action of the flocculant, and finally by sedimentation, filtration and other processes Remove it. [0003] For a long time, there are two main types of flocculants used in water supply and wastewater treatment: inorganic flocculants represented by aluminum salts, iron salts and their hydrolyzed polymers, and organic polymer flocculants represented by polyacrylamide and its derivatives. agent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/56C12R1/01
CPCC02F1/5263C02F1/56C12N1/20C12N1/205C12R2001/01
Inventor 穆军崔霞王玉霞阳广凤杨娇
Owner ZHEJIANG OCEAN UNIV
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