Method for rapidly detecting malachite green or crystal violet in aquaculture water

A technology of malachite green and aquaculture, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high cost, unsuitable on-site detection, and long time consumption, and achieve the effect of high accuracy, broad market application prospect, and easy operation

Inactive Publication Date: 2013-05-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods require the use of large instruments such as HPLC and MS, which are costly and time-consuming, and are not suitable for on-site detection
[0004] Rapid collaborative extraction technology is a new type of environmentally friendly solute enrichment and ...

Method used

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  • Method for rapidly detecting malachite green or crystal violet in aquaculture water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: The method for quickly detecting malachite green in aquaculture water is as follows:

[0018] ① Put 5 mL of eel culture water and 5 mL of malachite green standard series solutions with concentrations of 0.05, 0.1, 0.15, and 0.2 μg / mL respectively into 10 mL centrifuge tubes with stoppers, and add 300 μL isooctyl alcohol and 10 μL polysaccharide to each centrifuge tube. Ethylene glycol octyl phenyl ether Triton-100 (volume percent concentration: 10%); vortex for 5s to form a uniform emulsified system; centrifuge at 3500 r / min for 3 min, demulsification, phase separation, extractant A layer of ring is formed on the upper part of the centrifuge tube, and malachite green is extracted into this layer of ring; ②Under the same conditions, a layer of blue-green ring formed by the eel culture water on the upper part of the centrifuge tube and a layer of color formed by the standard series solution Compared with the ring color scale, the color of the color ring formed...

Embodiment 2

[0019] Example 2: The method for rapidly detecting crystal violet in aquaculture water, the specific operations are as follows:

[0020] ①Put 5mL of carp breeding water and 5mL of crystal violet standard series solutions with concentrations of 0.05, 0.1, and 0.15μg / mL respectively into 10ml stoppered centrifuge tubes, and add 200μl octanol and 50μl nonylphenol polyoxygen to each centrifuge tube Vinyl ether Np-7 (volume percent concentration: 5%), vortex for 20s to form a homogeneous emulsified system; centrifuge at 4000 r / min for 5min, demulsify, separate phases, and the extractant forms a ring on the upper part of the centrifuge tube , crystal violet is extracted into this ring; ②Under the same conditions, compare the purple ring formed by the carp culture water on the upper part of the centrifuge tube with the color ring formed by the standard series of solutions, the color of the color ring formed by the carp culture water It is lighter than the color formed by 0.05μg / mL ...

Embodiment 3

[0021] Example 3: The method for quickly detecting malachite green in aquaculture water is as follows:

[0022] ① Put 5 mL of shrimp culture water and 5 mL of malachite green standard series solutions with concentrations of 0.05, 0.1, 0.15, and 0.2 μg / mL respectively into centrifuge tubes with stoppers, and add 500 μl of Ren alcohol and 20 μl of branched-chain secondary to each centrifuge tube. Alcohol polyoxyethylene ether TMN-6 (volume percent concentration: 10%); vortex for 15s to form a uniform emulsified system; centrifuge at 2500 r / min for 6min to break the emulsion and separate phases, and the extractant is in the upper part of the centrifuge tube A ring is formed, and malachite green is extracted into this layer of ring; ②Under the same conditions, compare the color scale of a layer of blue-green ring formed by the shrimp culture water on the upper part of the centrifuge tube and a layer of color ring formed by the standard series solution , the color of the ring for...

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Abstract

The invention discloses a method for rapidly detecting malachite green or crystal violet in aquaculture water, which comprises the steps of using a rapid synergic solvent extraction system formed by auxiliary extraction agent, extraction agent and water to extract the crystal violet or the malachite green into organic phase at the upper part of a centrifuge tube; and detecting the malachite green or the crystal violet in the sample by comparing the standard content of the malachite green or the crystal violet, wherein the limit of detection is 0.05mu g/mL. Compared with the existing detection methods, the method is mild in extraction conditions and rapid in detection; the whole analysis and detection process can be completed within 5-10min; and the method is low in cost, does not need a large instrument and is high in accuracy, thus being suitable for detecting the safety of edible aquatic products and having great utilization potentiality and wide market prospect.

Description

technical field [0001] The invention relates to a method for quickly detecting malachite green or crystal violet in aquaculture water, which belongs to the detection technology of banned additives for food animals. Background technique [0002] Both malachite green and crystal violet belong to triphenylmethane dyes, which are bright in color and low in cost, and are widely used in aquaculture, food, medical and textile industries. With the development of science and technology, people have gradually discovered the harm of these two compounds to the human body. Because malachite green and crystal violet have bactericidal and anti-parasitic effects, they can prevent various fish diseases and are widely used in aquaculture. However, both itself and its metabolites have carcinogenic, teratogenic, mutagenic, chromosome breakage and respiratory system toxicity. Malachite green and crystal violet have been banned for use in aquaculture by the EU and US FDA. In order to avoid har...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 杨亚玲王佩瑾焦扬
Owner KUNMING UNIV OF SCI & TECH
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