Fermentation method of bacillus megatherium with high rate of sporation

Abstract

The invention relates to a fermentation method of bacillus megatherium with the high rate of sporation. The method comprises the following steps that 1, the bacillus megatherium is inoculated into a solid medium after being activated, static culture is conducted, and secondary activated bacillus megatherium is obtained; 2, the secondary activated bacillus megatherium is inoculated into a seed medium, fermentation culture is conducted, and seed bacterial liquid is prepared; 3, the seed bacterial liquid is inoculated into a fermentation medium, fermentation culture is conducted, supplementary food liquid is added, fermentation is conducted continuously, and bacillus megatherium fermentation liquor is prepared. According to the fermentation method of the bacillus megatherium with the high rate of sporation, culture conditions and medium components are selected, and meanwhile sporation accelerants of bacillus megatherium are added in the fermentation process, the viable count and the rate of sporation of the bacillus megatherium are increased, and the viable count in the prepared fermentation liquor can reach above 8.0*109 CFU / ml; by means of oil lens observation after crystal violet staining, the rate of sporation is above 95 percent, the spore count of a product can be significantly increased, and the product quality of the product can be significantly improved.

Description

technical field [0001] The invention relates to a method for fermenting Bacillus megaterium with a high spore rate, and belongs to the technical field of microbial fermentation engineering. Background technique [0002] Bacillus megaterium (Bacillus megaterium) is a Gram-positive bacterium, a plant root growth-promoting bacterium, and a common bacterial species in microbial fertilizers. Bacillus megaterium can be used to produce phosphorus-dissolving, potassium-dissolving, and nitrogen-fixing fertilizers. It has the effect of degrading phosphorus and potassium in the soil that cannot be used by plants. Applying it to tobacco leaves has a unique effect on improving the fermentation and aroma of tobacco leaves. It is not only a common strain for the production of microbial fertilizers, but also a common strain for the production of water treatment agents. Bacillus megaterium has many benefits to agricultural production, so it has broad application prospects in agriculture. B...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the deficiencies of the Bacillus megaterium fermentation technology commonly used in the existing microbial fertilizers, resulting in the problem that the spore yield is not high, and provides a Bacillus megaterium fermentation method with a high spore rate to improve the spore content of the product, product quality and use. Effect