Method for designing and preparing electrochemical detection-microfluidic multichannel chip based on self-assembled monolayer technology
A self-assembly technology and microfluidic channel technology, which is applied in the field of electrochemical detection-microfluidic multi-channel chip design and preparation, can solve the problem of single application of microelectrodes, achieve small sample consumption, expand application fields, cost reduction effect
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[0038] Example 1
[0039] Using self-assembled L-cysteine to detect Cu in solution 2+ Ion concentration.
[0040] L-Cysteine (L-CySteine) is one of the 20 important amino acids in nature and has good electrochemical activity. At the same time, the L-cysteine molecule contains a very active sulfo group (-SH), which is easy to be adsorbed on the surface of the gold electrode by forming Au-S bonds to form a self-assembled film. According to many literature reports, L-cysteine has an effect on Cu 2+ Ions have a very good coordination effect, thereby generating selective electrical signals.
[0041] In this experiment, L-cysteine was self-assembled on the surface of the gold electrode through the microchannel, and the Cu 2+ The test solution of ions is electrochemically detected. This experiment uses 8-electrode chip and 8-electrode microchannel.
[0042] Electrode cleaning: pass 0.5M H into the microchannel 2 SO 4 Solution, carry out cyclic voltammetry scanning in the range of...
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[0047] Example 2
[0048] A self-assembled sulfhydryl DNA probe was used to detect its characteristic sequence, and methylene blue (MB) was used as an indicator.
[0049] In the DNA electrochemical biosensor, a single-stranded DNA molecule (denoted as a DNA probe) with a known sequence is immobilized on an electrode, and the target DNA (cDNA) sequence in the sample is selectively identified according to the principle of base complementary pairing. The hybridization process is detected by electrochemical methods. This experiment uses MB as an indicator, because MB has a special affinity for guanine in single-stranded DNA (ssDNA), and its interaction with guanine reduces the electron mediator, resulting in a decrease in peak current; but when hybridized to form a double When stranded DNA (dsDNA), free guanine is no longer bound to MB on the electrode surface, and the peak current increases significantly. Therefore, the detection of characteristic DNA sequences can be achieved throu...
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